HSP90 / Heat Shock Protein 90 alpha Antibody (3B6)

HSP90 / Heat Shock Protein 90 alpha Monoclonal Antibody for Western blot, IF, IHC (P)

>> See 12 other antibodies for HSP90AA1 (HSP86; HSP89A; HSP90A; HSP90N; HSPC1; HSPCA; HSPCAL1; HSPCAL4; HSPN; Hsp89; Hsp90; LAP2; HSP 86; heat shock 86 kDa; heat shock 90kD protein 1; alpha; heat shock 90kD protein 1; alpha-like 4; heat shock 90kD protein; alpha-like 4; heat shock 90kDa protein 1; alpha; heat shock protein HSP 90-alpha; renal carcinoma antigen NY-REN-38)
Synonyms:
HSP86; HSP89A; HSP90A; HSP90N; HSPC1; HSPCA; HSPCAL1; HSPCAL4; HSPN; Hsp89; Hsp90; LAP2; HSP 86; heat shock 86 kDa; heat shock 90kD protein 1; alpha; heat shock 90kD protein 1; alpha-like 4; heat shock 90kD protein; alpha-like 4; heat shock 90kDa protein 1; alpha; heat shock protein HSP 90-alpha; renal carcinoma antigen NY-REN-38
Details
Host / Isotype: Mouse / IgG2b
Class: Monoclonal
Type: Antibody
Clone: 3B6
Tested Species Reactivity: Human (Hu), Mouse (Ms), Rat (Rt), Bovine (Bv)
Published Species Reactivity: Bovine (Bv), Chicken (Ck), Human (Hu), Mouse (Ms), Non-human primate (Nhp), Rabbit (Rb)
Immunogen: Purified mouse HSP90.
Ordering Information
Pierce HSP90 / Heat Shock Protein 90 alpha Antibody (3B6)
Product #
MA3-010
Size
100 µl
Price
$316.00
Purchase
Add HSP90 / Heat Shock Protein 90 alpha Antibody (3B6) to your cart.
Tested Applications Dilution *
Western Blot (WB) 1:500
Immunofluorescence (IF) 1:100-1:200
Immunohistochemistry (Paraffin) (IHC (P)) 1:20
Published Applications Dilution
Western Blot (WB) See publications below
Immunocytochemistry (ICC) See publications below
Immunohistochemistry (IHC) See publications below
Immunoprecipitation (IP) See publications below
* Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own experiment using appropriate negative and positive controls.
Form Information
Form: Liquid
Storage Buffer: ascites diluted in PBS
Preservative: 0.05% sodium azide
Storage Conditions: -20° C, Avoid Freeze/Thaw Cycles
Product Specific Information
MA3-010 detects heat shock protein 90 kDa (HSP90) from human, mouse, rat, and bovine tissues. This antibody does not detect chicken HSP90.

MA3-010 has been successfully used in Western blot, immunofluorescence, and immunohistochemistry (paraffin) procedures. By Western blot, this antibody detects a 90 kDa protein representing HSP90 from MCF7 cell extract. MA3-011 is more sensitive in Western blot procedures and should be used when maximum sensitivity is desired. MA3-010 does not immunoprecipitate HSP90 in any form (free or complexed).

The MA3-010 antigen is purified mouse HSP90.
General Information
Heat shock proteins (HSP) are proteins that are expressed in response to various biological stress, including heat. HSP90 is a 90 kDa protein that is induced under stress conditions, but is also one of the most abundant cellular proteins found under non-stress conditions. HSP90 has been found to be associated with a number of other intracellular proteins, including steroid receptors, actin, tubulin, and some kinases. It has been suggested that HSP90 is necessary to maintain structural integrity of at least some steroid receptors to allow for ligand binding during receptor activation.
Product Images
  • HSP90 / Heat Shock Protein 90 alpha Antibody (MA3-010) in WB
    HSP90_Antibody_MA3-010_Western_Blot_1.jpg

    Western Blot with anti-HSP90  /  Heat Shock Protein 90 alpha Monoclonal Antibody [3B6] (MA3-010) (HSP90_Antibody_MA3-010_Western_Blot_1.jpg)

    Western Blot with anti-HSP90 / Heat Shock Protein 90 alpha Monoclonal Antibody [3B6] (MA3-010)

    Western blot analysis of Heat Shock Protein 90 (HSP90) was performed by loading 50ug of various whole cell lysates and 15ul of PageRuler Prestained Protein Ladder (Product # 26616) onto a 4-20% Tris-HCl polyacrylamide gel. Proteins were transferred to a PVDF membrane and blocked with 5% BSA/TBST for at least 1 hour. The membrane was probed with an HSP90 monoclonal antibody (Product # MA3-010) at a dilution of 1:500 overnight at 4ºC on a rocking platform, washed in TBS-0.1%Tween-20, and probed with a goat anti-mouse IgG-HRP secondary antibody (Product # 31430) at a dilution of 1:20,000 for at least 1 hour. Chemiluminescent detection was performed using SuperSignal West Dura (Product # 34075).
  • HSP90 / Heat Shock Protein 90 alpha Antibody (MA3-010) in IF
    MA3-010_IF_3T3.jpg

    Immunofluorescence with anti-HSP90  /  Heat Shock Protein 90 alpha Monoclonal Antibody [3B6] (MA3-010) (MA3-010_IF_3T3.jpg)

    Immunofluorescence with anti-HSP90 / Heat Shock Protein 90 alpha Monoclonal Antibody [3B6] (MA3-010)

    Immunofluorescent analysis of Heat Shock Protein 90 using Heat Shock Protein 90 Monoclonal antibody (3B6) (Product# MA3-010) shows staining in NIH-3T3 cells. Heat Shock Protein 90 staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with or an antibody recognizing Heat Shock Protein 90 (Product# MA3-010) at a dilution of 1:100-1:200 over night at 4ºC, washed with PBS and incubated with a DyLight-488 conjugated secondary antibody (Product# 35552 for GAR, Product# 35503 for GAM). Images were taken at 60X magnification.
  • HSP90 / Heat Shock Protein 90 alpha Antibody (MA3-010) in IF
    MA3-010_IF_Hela.jpg

    Immunofluorescence with anti-HSP90  /  Heat Shock Protein 90 alpha Monoclonal Antibody [3B6] (MA3-010) (MA3-010_IF_Hela.jpg)

    Immunofluorescence with anti-HSP90 / Heat Shock Protein 90 alpha Monoclonal Antibody [3B6] (MA3-010)

    Immunofluorescent analysis of Heat Shock Protein 90 using Heat Shock Protein 90 Monoclonal antibody (3B6) (Product# MA3-010) shows staining in HeLa cells. Heat Shock Protein 90 staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with or an antibody recognizing Heat Shock Protein 90 (Product# MA3-010) at a dilution of 1:100-1:200 over night at 4ºC, washed with PBS and incubated with a DyLight-488 conjugated secondary antibody (Product# 35552 for GAR, Product# 35503 for GAM). Images were taken at 60X magnification.
  • HSP90 / Heat Shock Protein 90 alpha Antibody (MA3-010) in IF
    MA3-010_IF_MCF-7.jpg

    Immunofluorescence with anti-HSP90  /  Heat Shock Protein 90 alpha Monoclonal Antibody [3B6] (MA3-010) (MA3-010_IF_MCF-7.jpg)

    Immunofluorescence with anti-HSP90 / Heat Shock Protein 90 alpha Monoclonal Antibody [3B6] (MA3-010)

    Immunofluorescent analysis of Heat Shock Protein 90 using Heat Shock Protein 90 Monoclonal antibody (3B6) (Product# MA3-010) shows staining in MCF-7 cells. Heat Shock Protein 90 staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with or an antibody recognizing Heat Shock Protein 90 (Product# MA3-010) at a dilution of 1:100-1:200 over night at 4ºC, washed with PBS and incubated with a DyLight-488 conjugated secondary antibody (Product# 35552 for GAR, Product# 35503 for GAM). Images were taken at 60X magnification.
  • HSP90 / Heat Shock Protein 90 alpha Antibody (MA3-010) in IF
    HSP90_Antibody_MA3-010_Immunofluorescence_4.jpg

    Immunofluorescence with anti-HSP90  /  Heat Shock Protein 90 alpha Monoclonal Antibody [3B6] (MA3-010) (HSP90_Antibody_MA3-010_Immunofluorescence_4.jpg)

    Immunofluorescence with anti-HSP90 / Heat Shock Protein 90 alpha Monoclonal Antibody [3B6] (MA3-010)

    Immunofluorescent analysis of Heat Shock Protein 90 (HSP90, green) in HeLa cells. Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA (Product # 37525) for 15 minutes at room temperature. Cells were probed without (left panel) or with (right panel) an HSP90 monoclonal antibody (Product # MA3-010) at a dilution of 1:100 for at least 1 hour at room temperature, washed with PBS, and incubated with DyLight 488 goat anti-mouse IgG secondary antibody (Product # 35502) at a dilution of 1:400 for 30 minutes at room temperature. F-Actin (red) was stained with DyLight 554 Phalloidin (Product # 21834) and nuclei (blue) were stained with Hoechst 33342 dye (Product # 62249). Images were taken on a Thermo Scientific ArrayScan or a ToxInsight Instrument at 20X magnification.
  • HSP90 / Heat Shock Protein 90 alpha Antibody (MA3-010) in IHC (P)
    MA3-010_Immunohistochemistry_Breast carcinoma.jpg

    Immunohistochemistry (Paraffin) with anti-HSP90  /  Heat Shock Protein 90 alpha Monoclonal Antibody [3B6] (MA3-010) (MA3-010_Immunohistochemistry_Breast carcinoma.jpg)

    Immunohistochemistry (Paraffin) with anti-HSP90 / Heat Shock Protein 90 alpha Monoclonal Antibody [3B6] (MA3-010)

    Immunohistochemistry was performed on cancer biopsies of deparaffinized Human breast carcinoma tissues. To expose target proteins, heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes. Following antigen retrieval tissues were blocked in 3% BSA-PBS for 30 minutes at room temperature. Tissues were then probed at a dilution of 1:20 with a mouse monoclonal antibody recognizing Heat Shock Protein 90 (MA3-010) or without primary antibody (negative control) overnight at 4°C in a humidified chamber. Tissues were washed extensively with PBST and endogenous peroxidase activity was quenched with a peroxidase suppressor. Detection was performed using a biotin-conjugated secondary antibody and SA-HRP, followed by colorimetric detection using DAB. Tissues were counterstained with hematoxylin and prepped for mounting.
  • HSP90 / Heat Shock Protein 90 alpha Antibody (MA3-010) in IHC (P)
    MA3-010_Immunohistochemistry_Kidney tissue.jpg

    Immunohistochemistry (Paraffin) with anti-HSP90  /  Heat Shock Protein 90 alpha Monoclonal Antibody [3B6] (MA3-010) (MA3-010_Immunohistochemistry_Kidney tissue.jpg)

    Immunohistochemistry (Paraffin) with anti-HSP90 / Heat Shock Protein 90 alpha Monoclonal Antibody [3B6] (MA3-010)

    Immunohistochemistry was performed on normal deparaffinized Human kidney tissue. To expose target proteins, heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes. Following antigen retrieval tissues were blocked in 3% BSA-PBS for 30 minutes at room temperature. Tissues were then probed at a dilution of 1:20 with a mouse monoclonal antibody recognizing Heat Shock Protein 90 (MA3-010) or without primary antibody (negative control) overnight at 4°C in a humidified chamber. Tissues were washed extensively with PBST and endogenous peroxidase activity was quenched with a peroxidase suppressor. Detection was performed using a biotin-conjugated secondary antibody and SA-HRP, followed by colorimetric detection using DAB. Tissues were counterstained with hematoxylin and prepped for mounting.
  • HSP90 / Heat Shock Protein 90 alpha Antibody (MA3-010) in IHC (P)
    MA3-010_Immunohistochemistry_tonsil tissue.jpg

    Immunohistochemistry (Paraffin) with anti-HSP90  /  Heat Shock Protein 90 alpha Monoclonal Antibody [3B6] (MA3-010) (MA3-010_Immunohistochemistry_tonsil tissue.jpg)

    Immunohistochemistry (Paraffin) with anti-HSP90 / Heat Shock Protein 90 alpha Monoclonal Antibody [3B6] (MA3-010)

    Immunohistochemistry was performed on normal deparaffinized Human tonsil tissue. To expose target proteins, heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes. Following antigen retrieval tissues were blocked in 3% BSA-PBS for 30 minutes at room temperature. Tissues were then probed at a dilution of 1:20 with a mouse monoclonal antibody recognizing Heat Shock Protein 90 (MA3-010) or without primary antibody (negative control) overnight at 4°C in a humidified chamber. Tissues were washed extensively with PBST and endogenous peroxidase activity was quenched with a peroxidase suppressor. Detection was performed using a biotin-conjugated secondary antibody and SA-HRP, followed by colorimetric detection using DAB. Tissues were counterstained with hematoxylin and prepped for mounting.
  • HSP90 / Heat Shock Protein 90 alpha Antibody (MA3-010) in IHC (P)
    Hsp90_Antibody_MA3-010_Immunohistochemistry_4_20130515080307.jpg

    Immunohistochemistry (Paraffin) with anti-HSP90  /  Heat Shock Protein 90 alpha Monoclonal Antibody [3B6] (MA3-010) (Hsp90_Antibody_MA3-010_Immunohistochemistry_4_20130515080307.jpg)

    Immunohistochemistry (Paraffin) with anti-HSP90 / Heat Shock Protein 90 alpha Monoclonal Antibody [3B6] (MA3-010)

    Immunohistochemistry was performed on paraffin-embedded human colon cancer tissue sections. To expose target proteins, heat-induced epitope retrieval was performed using 10mM sodium citrate (pH 6.0) buffer for 20 minutes at 95ºC. Following antigen retrieval, tissues were blocked in 3% BSA (Product # 37525) in PBST for 30 minutes at room temperature and then probed with a Heat Shock Protein 90 (Hsp90) monoclonal antibody (Product # MA3-010) at a dilution of 1:100 for 1 hour in a humidified chamber (right panel). As a negative control, the primary antibody was eliminated from the staining procedure (left panel). Tissues were washed extensively with PBS/0.025% Tween-20 (Product # 28314) and endogenous peroxidase activity quenched with Peroxidase Suppressor (Product # 35000) for 30 minutes at room temperature. Detection was performed using an HRP-conjugated goat anti-mouse IgG-HRP secondary antibody (Product # 31431) at a dilution of 1:250 followed by colorimetric detection using Metal Enhanced DAB Substrate Kit (Product # 34065). Tissues were counterstained with hematoxylin and prepped for mouting. Images were taken on a Zeiss Axiovision microscope at 40X magnification (x1.6 Optovar).
Publications:
Western Blot
  Species / Dilution Summary
  Bv / 1:100
MA3-010 was used in western blot to investigate the differences of HSP90 expression in cow mammary tissue at various lactation stages

J Dairy Sci. 1997 Oct;80(10):2372-9.
"Effect of stages of lactation on the concentration of a 90-kilodalton heat shock protein in bovine mammary tissue."
Author(s): Watanabe A, Miyamoto T, Katoh N, Takahashi Y
Number of Citations: 1
(See PubMed article )
  Ck / Not Cited
MA3-010 was used in immunocytochemistry and western blot to perform mutational analysis of HSP90 alpha and study the corresponding influence on its dimerization and cellular distribution

J Cell Sci. 1996 Jul;109 ( Pt 7)():1677-87.
"Mutational analysis of Hsp90 alpha dimerization and subcellular localization: dimer disruption does not impede "in vivo' interaction with estrogen receptor."
Author(s): Meng X, Devin J, Sullivan WP, Toft D, Baulieu EE, Catelli MG
Number of Citations: 9
(See PubMed article )
  Hu / Not Cited
MA3-010 was used in western blot to study the role of cell-cell interactions in the ligand-independent nuclear translocation of the glucocorticoid receptor

J Steroid Biochem Mol Biol. 1996 Feb;57(3-4):239-49.
"Agonist-free transformation of the glucocorticoid receptor in human B-lymphoma cells."
Author(s): van den Berg JD, Smets LA, van Rooij H
Number of Citations: 0
(See PubMed article )
  Hu / 1:500
MA3-010 was used in immunocytochemistry, immunoprecipitation and western blot to study the molecular mechanism of UDCA action.

J Biol Chem. 2001 Dec 14;276(50):47371-8.
"Functional modulation of the glucocorticoid receptor and suppression of NF-kappaB-dependent transcription by ursodeoxycholic acid."
Author(s): Miura T, Ouchida R, Yoshikawa N, Okamoto K, Makino Y, Nakamura T, Morimoto C, Makino I, Tanaka H
Number of Citations: 1
(See PubMed article )
  Ms / 1:300
MA3-010 was used in western blot to investigate the glucocorticoid receptor status of murine S49 lymphoma cells exposed to dexamethasone

J Steroid Biochem Mol Biol. 1994 Oct;51(1-2):33-40.
"High levels of non-activated receptors in glucocorticoid-sensitive S49wt mouse lymphoma cells incubated with dexamethasone."
Author(s): van den Berg JD, Smets LA, Hutchison KA, van Rooij H, van den Elshout MM
Number of Citations: 0
(See PubMed article )
  Nhp / 1:500
MA3-010 was used in western blot and immunoprecipitation to investigate the regulatory role of cortivazol on glucocorticoid receptor.

J Biol Chem. 2002 Feb 15;277(7):5529-40.
"Distinct interaction of cortivazol with the ligand binding domain confers glucocorticoid receptor specificity: cortivazol is a specific ligand for the glucocorticoid receptor."
Author(s): Yoshikawa N, Makino Y, Okamoto K, Morimoto C, Makino I, Tanaka H
Number of Citations: 1
(See PubMed article )
  Nhp / 1:500
MA3-010 was used in western blot to study the mechanism for differential actions of various synthetic steroids on glucocorticoid receptor.

Mol Endocrinol. 2005 May;19(5):1110-24.
"The distinct agonistic properties of the phenylpyrazolosteroid cortivazol reveal interdomain communication within the glucocorticoid receptor."
Author(s): Yoshikawa N, Yamamoto K, Shimizu N, Yamada S, Morimoto C, Tanaka H
Number of Citations: 1
(See PubMed article )
  Rb / Not Cited
MA3-010 was used in western blot to investigate the role of chaperone components for energy-dependent protein refolding activity

FEBS Lett. 1993 Sep 27;331(1-2):25-30.
"ATP-dependent protein refolding activity in reticulocyte lysate. Evidence for the participation of different chaperone components."
Author(s): Nimmesgern E, Hartl FU
Number of Citations: 7
(See PubMed article )
Immunocytochemistry
  Species / Dilution Summary
  Ck / 1:2,000
MA3-010 was used in immunocytochemistry and western blot to perform mutational analysis of HSP90 alpha and study the corresponding influence on its dimerization and cellular distribution

J Cell Sci. 1996 Jul;109 ( Pt 7)():1677-87.
"Mutational analysis of Hsp90 alpha dimerization and subcellular localization: dimer disruption does not impede "in vivo' interaction with estrogen receptor."
Author(s): Meng X, Devin J, Sullivan WP, Toft D, Baulieu EE, Catelli MG
Number of Citations: 9
(See PubMed article )
  Hu / 2 ug/ml
MA3-010 was used in immunocytochemistry, immunoprecipitation and western blot to study the molecular mechanism of UDCA action.

J Biol Chem. 2001 Dec 14;276(50):47371-8.
"Functional modulation of the glucocorticoid receptor and suppression of NF-kappaB-dependent transcription by ursodeoxycholic acid."
Author(s): Miura T, Ouchida R, Yoshikawa N, Okamoto K, Makino Y, Nakamura T, Morimoto C, Makino I, Tanaka H
Number of Citations: 1
(See PubMed article )
Immunohistochemistry
  Species / Dilution Summary
  Ms / 1:100
MA3-010 was used in immunohistochemistry to identify highly abundant heat shock proteins and chaperone proteins in mature mouse egg proteome.

Reprod Biol Endocrinol. 2003 Feb 14;1():27.
"Oolemmal proteomics--identification of highly abundant heat shock proteins and molecular chaperones in the mature mouse egg and their localization on the plasma membrane."
Author(s): Calvert ME, Digilio LC, Herr JC, Coonrod SA
Number of Citations: 11
(See PubMed article )
Immunoprecipitation
  Species / Dilution Summary
  Hu / Not Cited
MA3-010 was used in immunocytochemistry, immunoprecipitation and western blot to study the molecular mechanism of UDCA action.

J Biol Chem. 2001 Dec 14;276(50):47371-8.
"Functional modulation of the glucocorticoid receptor and suppression of NF-kappaB-dependent transcription by ursodeoxycholic acid."
Author(s): Miura T, Ouchida R, Yoshikawa N, Okamoto K, Makino Y, Nakamura T, Morimoto C, Makino I, Tanaka H
Number of Citations: 1
(See PubMed article )
  Nhp / Not Cited
MA3-010 was used in western blot and immunoprecipitation to investigate the regulatory role of cortivazol on glucocorticoid receptor.

J Biol Chem. 2002 Feb 15;277(7):5529-40.
"Distinct interaction of cortivazol with the ligand binding domain confers glucocorticoid receptor specificity: cortivazol is a specific ligand for the glucocorticoid receptor."
Author(s): Yoshikawa N, Makino Y, Okamoto K, Morimoto C, Makino I, Tanaka H
Number of Citations: 1
(See PubMed article )
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