HSD3B, HSDB, SDR11E2, 3 beta-HSD type II, 3 beta-hydroxysteroid dehydrogenase type II, delta 5-delta 4-isomerase type II, 3 beta-HSD type II, 3 beta-hydroxysteroid dehydrogenase/Delta 5-->4-isomerase type 2, 3 beta-hydroxysteroid dehydrogenase/Delta 5-->4-isomerase type II, 3-beta
* Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own experiment using appropriate negative and positive controls.
Antigen affinity chromatography
0.1M tris, pH 7,
10% glycerol, 0.1M glycine
-20° C, Avoid Freeze/Thaw Cycles
Product Specific Information
PA5-27791 targets HSD3B2 in IF, IHC (P), and WB applications and shows reactivity with Human samples.
The PA5-27791 immunogen is recombinant fragment corresponding to a region within amino acids 126 and 372 of Human HSD3B2.
The protein encoded by this gene is a bifunctional enzyme that catalyzes the oxidative conversion of delta(5)-ene-3-beta-hydroxy steroid, and the oxidative conversion of ketosteroids. It plays a crucial role in the biosynthesis of all classes of hormonal steroids. This gene is predominantly expressed in the adrenals and the gonads. Mutations in this gene are associated with 3-beta-hydroxysteroid dehydrogenase, type II, deficiency. Alternatively spliced transcript variants have been found for this gene.
HSD3B2 Antibody (PA5-27791) in WB PA5-27791_WB.jpg
Western Blot with anti-HSD3B2 Polyclonal Antibody (PA5-27791)
Western blot analysis of HSD3B2 using A) 30 µg HeLa whole cell lysate and B) 30 µg Raji whole cell lysate. Samples were loaded onto a 10% SDS-PAGE gel and probed with a HSD3B2 polyclonal antibody (Product # PA5-27791) at a dilution of 1:500.
HSD3B2 Antibody (PA5-27791) in IF PA5-27791_IF.jpg
Immunofluorescence with anti-HSD3B2 Polyclonal Antibody (PA5-27791)