HSD3B2 Antibody

HSD3B2 Polyclonal Antibody for Western blot, IF, ICC, IHC (P)

>> See 1 other antibody for HSD3B2 (HSD3B, HSDB, SDR11E2, 3 beta-HSD type II, 3 beta-hydroxysteroid dehydrogenase type II, delta 5-delta 4-isomerase type II, 3 beta-HSD type II, 3 beta-hydroxysteroid dehydrogenase/Delta 5-->4-isomerase type 2, 3 beta-hydroxysteroid dehydrogenase/Delta 5-->4-isomerase type II, 3-beta)
Synonyms:
HSD3B, HSDB, SDR11E2, 3 beta-HSD type II, 3 beta-hydroxysteroid dehydrogenase type II, delta 5-delta 4-isomerase type II, 3 beta-HSD type II, 3 beta-hydroxysteroid dehydrogenase/Delta 5-->4-isomerase type 2, 3 beta-hydroxysteroid dehydrogenase/Delta 5-->4-isomerase type II, 3-beta
Entrez Gene ID:
UniProt ID:
Details
Host / Isotype: Rabbit / IgG
Class: Polyclonal
Type: Antibody
Tested Species Reactivity: Human (Hu)
Immunogen: Recombinant fragment corresponding to a region within amino acids 126 and 372 of Human HSD3B2
Ordering Information
Pierce HSD3B2 Antibody
Product #
PA5-27791
Size
100 µL
Price
$324.00
Purchase
Tested Applications Dilution *
Western Blot (WB) 1:500-1:3000
Immunofluorescence (IF) 1:100-1:1000
Immunocytochemistry (ICC) 1:100-1:1000
Immunohistochemistry (Paraffin) (IHC (P)) 1:100-1:1000
* Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own experiment using appropriate negative and positive controls.
Form Information
Form: Liquid
Concentration: 0.3mg/ml
Purification: Antigen affinity chromatography
Storage Buffer: 0.1M tris, pH 7, with 10% glycerol, 0.1M glycine
Preservative: 0.01% thimerosal
Storage Conditions: -20° C, Avoid Freeze/Thaw Cycles
Product Specific Information
PA5-27791 targets HSD3B2 in IF, IHC (P), and WB applications and shows reactivity with Human samples.

The PA5-27791 immunogen is recombinant fragment corresponding to a region within amino acids 126 and 372 of Human HSD3B2.
General Information
The protein encoded by this gene is a bifunctional enzyme that catalyzes the oxidative conversion of delta(5)-ene-3-beta-hydroxy steroid, and the oxidative conversion of ketosteroids. It plays a crucial role in the biosynthesis of all classes of hormonal steroids. This gene is predominantly expressed in the adrenals and the gonads. Mutations in this gene are associated with 3-beta-hydroxysteroid dehydrogenase, type II, deficiency. Alternatively spliced transcript variants have been found for this gene.
Product Images
  • HSD3B2 Antibody (PA5-27791) in WB
    PA5-27791_WB.jpg

    Western Blot with anti-HSD3B2 Polyclonal Antibody (PA5-27791) (PA5-27791_WB.jpg)

    Western Blot with anti-HSD3B2 Polyclonal Antibody (PA5-27791)

    Western blot analysis of HSD3B2 using A) 30 µg HeLa whole cell lysate and B) 30 µg Raji whole cell lysate. Samples were loaded onto a 10% SDS-PAGE gel and probed with a HSD3B2 polyclonal antibody (Product # PA5-27791) at a dilution of 1:500.
  • HSD3B2 Antibody (PA5-27791) in IF
    PA5-27791_IF.jpg

    Immunofluorescence with anti-HSD3B2 Polyclonal Antibody (PA5-27791) (PA5-27791_IF.jpg)

    Immunofluorescence with anti-HSD3B2 Polyclonal Antibody (PA5-27791)

    Immunofluorescent analysis of HSD3B2 in methanol-fixed HeLa cells using a HSD3B2 polyclonal antibody (Product # PA5-27791) at a 1:200 dilution.
  • HSD3B2 Antibody (PA5-27791) in IHC (P)
    PA5-27791_IHC_2.jpg

    Immunohistochemistry (Paraffin) with anti-HSD3B2 Polyclonal Antibody (PA5-27791) (PA5-27791_IHC_2.jpg)

    Immunohistochemistry (Paraffin) with anti-HSD3B2 Polyclonal Antibody (PA5-27791)

    Immunohistochemical analysis of HSD3B2 in the cytoplasm of paraffin-embedded mouse ovary tissue using a HSD3B2 polyclonal antibody (Product # PA5-27791).
  • HSD3B2 Antibody (PA5-27791) in IHC (P)
    PA5-27791_IHC_1.jpg

    Immunohistochemistry (Paraffin) with anti-HSD3B2 Polyclonal Antibody (PA5-27791) (PA5-27791_IHC_1.jpg)

    Immunohistochemistry (Paraffin) with anti-HSD3B2 Polyclonal Antibody (PA5-27791)

    Immunohistochemical analysis of HSD3B2 in paraffin-embedded A549 xenograft using a HSD3B2 polyclonal antibody (Product # PA5-27791) at a dilution of 1:100.
(This product is for In Vitro experimental use only. Not for resale without express authorization.)
Part of Thermo Fisher Scientific