HRP / Horseradish peroxidase Antibody (HP-03)

HRP / Horseradish peroxidase Monoclonal Antibody for Western blot, ICC, ELISA

Host / Isotype: Mouse / IgG1
Class: Monoclonal
Type: Antibody
Clone: HP-03
Tested Species Reactivity: Tag (Tag)
Immunogen: Horseradish peroxidase (Grade I)
Ordering Information
Pierce HRP / Horseradish peroxidase Antibody (HP-03)
Product #
100 ug
Tested Applications Dilution *
Western Blot (WB) Assay Dependent
Immunocytochemistry (ICC) Assay Dependent
ELISA (ELISA) Assay Dependent
* Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own experiment using appropriate negative and positive controls.
Form Information
Form: Liquid
Purification: selective precipitation
Storage Buffer: PBS, pH 7.4
Preservative: 15mM sodium azide
Storage Conditions: 4° C, do not freeze
Product Specific Information
General Information
Chemiluminescent detection systems have emerged as the best all-around method for detection of Western blots. They eliminate the hazards associated with radioactive materials and toxic chromogenic substrates. The speed and sensitivity of these methods are unequalled by traditional alternatives. Because results are generated on film, it is possible to record and store data permanently, and blots detected with chemiluminescent methods are easily stripped for subsequent reprobing with additional antibodies. HRP (Horseradish Peroxidase) conjugated secondary antibodies are utilized in conjunction with specific chemiluminescent substrates to generate the light signal. HRP-antibody conjugates have a very high turnover rate, giving good sensitivity with short reaction times.
(This product is for In Vitro experimental use only. Not for resale without express authorization.)
Part of Thermo Fisher Scientific