HPRT antibody

HPRT Polyclonal antibody for Western blot, IF, ICC, IHC (P)

>> See 4 other antibodies for HPRT1 (HGPRT, HPRT, HGPRTase, hypoxanthine-guanine phosphoribosyltransferase)
Synonyms:
HGPRT, HPRT, HGPRTase, hypoxanthine-guanine phosphoribosyltransferase
Entrez Gene ID:
UniProt ID:
Details
Host / Isotype: Rabbit / IgG
Class: Polyclonal
Type: antibody
Tested Species Reactivity: Human (Hu), Mouse (Ms)
Immunogen: Recombinant fragment corresponding to a region within amino acids 23 and 218 of HPRT (Uniprot ID#P00492)
Ordering Information
Pierce HPRT antibody
Product #
PA5-22281
Size
100 µL
Price
$324.00
Purchase
Tested Applications Dilution *
Western Blot (WB) 1:500-1:3000
Immunofluorescence (IF) 1:100-1:1000
Immunocytochemistry (ICC) 1:100-1:1000
Immunohistochemistry (Paraffin) (IHC (P)) 1:100-1:1000
* Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own experiment using appropriate negative and positive controls.
Form Information
Form: Liquid
Concentration: 0.95mg/ml
Purification: Antigen affinity chromatography
Storage Buffer: 0.1M tris, pH 7, with 20% glycerol, 0.1M glycine
Preservative: 0.01% thimerosal
Storage Conditions: -20° C, Avoid Freeze/Thaw Cycles
Product Specific Information
PA5-22281 targets HPRT in ICC, IF, IHC (P), and WB applications and shows reactivity with Human samples.

The PA5-22281 immunogen is a recombinant fragment corresponding to a region within amino acids 23 and 218 of HPRT (Uniprot ID#P00492).
General Information
Product Images
  • HPRT antibody (PA5-22281) in WB
    PA5-22281_WB_1.jpg

    Western Blot with anti-HPRT Polyclonal antibody (PA5-22281) (PA5-22281_WB_1.jpg)

    Western Blot with anti-HPRT Polyclonal antibody (PA5-22281)

    Western blot analysis of HPRT using 50 µg mouse liver lysate. Samples were loaded onto a 12% SDS-PAGE gel and probed with a HPRT polyclonal antibody (Product # PA5-22281) at a dilution of 1:500.
  • HPRT antibody (PA5-22281) in WB
    PA5-22281_WB_2.jpg

    Western Blot with anti-HPRT Polyclonal antibody (PA5-22281) (PA5-22281_WB_2.jpg)

    Western Blot with anti-HPRT Polyclonal antibody (PA5-22281)

    Western blot analysis of HPRT using 30µg of A) MOLT4 and B) Raji lysate. Samples were loaded onto a 12% SDS-PAGE gel and probed with a HPRT polyclonal antibody (Product # PA5-22281) at a dilution of 1:1000.
  • HPRT antibody (PA5-22281) in IF
    PA5-22281_IF.jpg

    Immunofluorescence with anti-HPRT Polyclonal antibody (PA5-22281) (PA5-22281_IF.jpg)

    Immunofluorescence with anti-HPRT Polyclonal antibody (PA5-22281)

    Immunofluorescent analysis of HPRT in paraformaldehyde-fixed HeLa cells using a HPRT polyclonal antibody (Product # PA5-22281) at a 1:200 dilution.
  • HPRT antibody (PA5-22281) in IHC (P)
    PA5-22281_IHC.jpg

    Immunohistochemistry (Paraffin) with anti-HPRT Polyclonal antibody (PA5-22281) (PA5-22281_IHC.jpg)

    Immunohistochemistry (Paraffin) with anti-HPRT Polyclonal antibody (PA5-22281)

    Immunohistochemical analysis of HPRT in paraffin-embedded NCI-N87 xenograft using a HPRT polyclonal antibody (Product # PA5-22281) at a dilution of 1:100.
(This product is for In Vitro experimental use only. Not for resale without express authorization.)
Part of Thermo Fisher Scientific