HPRT antibody

HPRT Polyclonal antibody for Western blot, IF, ICC, IHC (P)

>> See 4 other antibodies for HPRT1 (HGPRT, HPRT, HGPRTase, hypoxanthine-guanine phosphoribosyltransferase)
Synonyms:
HGPRT, HPRT, HGPRTase, hypoxanthine-guanine phosphoribosyltransferase
Entrez Gene ID:
UniProt ID:
Details
Host / Isotype: Rabbit / IgG
Class: Polyclonal
Type: antibody
Tested Species Reactivity: Human (Hu), Mouse (Ms)
Immunogen: Recombinant fragment corresponding to a region within amino acids 23 and 218 of HPRT (Uniprot ID#P00492)
Ordering Information
Pierce HPRT antibody
Product #
PA5-22281
Size
100 ul
Price
$316.00
Purchase
Add HPRT antibody to your cart.
Tested Applications Dilution *
Western Blot (WB) 1:500-1:3000
Immunofluorescence (IF) 1:100-1:1000
Immunocytochemistry (ICC) 1:100-1:1000
Immunohistochemistry (Paraffin) (IHC (P)) 1:100-1:1000
* Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own experiment using appropriate negative and positive controls.
Form Information
Form: Liquid
Concentration: 0.95mg/ml
Purification: Antigen affinity chromatography
Storage Buffer: 0.1M tris, pH 7, with 20% glycerol, 0.1M glycine
Preservative: 0.01% thimerosal
Storage Conditions: -20° C, Avoid Freeze/Thaw Cycles
Product Specific Information
PA5-22281 targets HPRT in ICC, IF, IHC (P), and WB applications and shows reactivity with Human samples.

The PA5-22281 immunogen is a recombinant fragment corresponding to a region within amino acids 23 and 218 of HPRT (Uniprot ID#P00492).
General Information
Product Images
  • HPRT antibody (PA5-22281) in WB
    PA5-22281_Western Blot.jpg

    Western Blot with anti-HPRT Polyclonal antibody (PA5-22281) (PA5-22281_Western Blot.jpg)

    Western Blot with anti-HPRT Polyclonal antibody (PA5-22281)

    Western blot analysis in whole cell lysates of (target) in lanes A). Molt-4 cells [30 ug] B). Raji cells [30 ug] using PA5-22281 anti-HPRT primary antibody at a dilution of 1:1000
  • HPRT antibody (PA5-22281) in IF
    PA5-22281_Immunofluorescence.jpg

    Immunofluorescence with anti-HPRT Polyclonal antibody (PA5-22281) (PA5-22281_Immunofluorescence.jpg)

    Immunofluorescence with anti-HPRT Polyclonal antibody (PA5-22281)

    Immunofluorescence analysis of paraformaldehyde-fixed HeLa cells using PA5-22281 anti-HPRT antibody at 1:200 dilution.
  • HPRT antibody (PA5-22281) in IHC
    PA5-22281_Immunohistochemistry.jpg

    Immunohistochemistry with anti-HPRT Polyclonal antibody (PA5-22281) (PA5-22281_Immunohistochemistry.jpg)

    Immunohistochemistry with anti-HPRT Polyclonal antibody (PA5-22281)

    Immunohistochemical analysis of paraffin-embedded NCI-N87 xenograft tissue using PA5-22281 anti-HPRT antibody at 1:100 dilution.
(This product is for In Vitro experimental use only. Not for resale without express authorization.)
Part of Thermo Fisher Scientific