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HMGA1 Antibody (PA5-19184) in WB
Western Blot with anti-HMGA1 Polyclonal Antibody (PA5-19184)
PA5-19184 (0.5µg/ml) in Human Pancreas lysate with (B) and without (A) blocking with the immunising peptide, the primary antibody incubation was 1 hour and detected by chemiluminescence.
HMGA1 Antibody
HMGA1 Polyclonal Antibody for Western blot
Synonyms:
HMGA1; high mobility group AT-hook 1; HMG-R; HMGA1A; HMGIY; MGC12816; MGC4242; MGC4854; OTTHUMP00000016222; OTTHUMP00000016223; OTTHUMP00000016224; OTTHUMP00000039618; high-mobility group (nonhistone chromosomal) protein isoforms I and Y; nonhistone chromosomal high-mobility group protein HMG-I/HMG-Y
Entrez Gene ID:
UniProt ID:
(Hu) P17096
| Details | |
| Host / Isotype: | Goat |
| Class: | Polyclonal |
| Type: | Antibody |
| Species Reactivity: | Human (Hu) |
| Immunogen: | Synthetic peptide sequence (SQPLASKQEKDGT) corresponding to the internal amino acids of HMGA1 |
| Ordering Information | ||||
| Pierce HMGA1 Antibody |
| Storage: | -20° C, Avoid Freeze/Thaw Cycles |
| Form: | 100 ug of purified antibody from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. Buffered in 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. |
| Applications | Dilution * |
| Western Blot (WB) | 0.5-1 ug/ml |
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* Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own experiment using appropriate negative and positive controls.
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| Product Specific Information |
| PA5-19184 targets HMGA1 in WB applications and shows reactivity with Human samples. The PA5-19184 immunogen is synthetic peptide sequence (SQPLASKQEKDGT) corresponding to the internal amino acids of HMGA1. |
| General Information |
| This gene encodes a non-histone protein involved in many cellular processes, including regulation of inducible gene transcription, integration of retroviruses into chromosomes, and the metastatic progression of cancer cells. The encoded protein preferentially binds to the minor groove of A+T-rich regions in double-stranded DNA. It has little secondary structure in solution but assumes distinct conformations when bound to substrates such as DNA or other proteins. The encoded protein is frequently acetylated and is found in the nucleus. At least seven transcript variants encoding two different isoforms have been found for this gene. |
(This product is for In Vitro experimental use only. Not for resale without express authorization.)
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HMGA1 Antibodies > HMGA1 Polyclonal Antibody PA5-19184
