HIF Prolylhydroxylase 2 Antibody

HIF Prolylhydroxylase 2 Polyclonal Antibody for Western blot, IF, ICC, IHC (P), FACS, IP

>> See 5 other antibodies for EGLN1 (C1ORF12, Chromosome 1 Open Reading Frame 12, EGL 9 homolog of C. elegans 1, EGLN 1 EGLN1, HIF Prolyl Hydroxylase 2, HIF-P4H 2, HIFPH2, P4H2, Prolyl Hy)
Synonyms:
C1ORF12, Chromosome 1 Open Reading Frame 12, EGL 9 homolog of C. elegans 1, EGLN 1 EGLN1, HIF Prolyl Hydroxylase 2, HIF-P4H 2, HIFPH2, P4H2, Prolyl Hy
Entrez Gene ID:
UniProt ID:
Details
Host / Isotype: Rabbit
Class: Polyclonal
Type: Antibody
Tested Species Reactivity: Human (Hu)
Immunogen: A synthetic peptide made to an N-terminal sequence of the human EGL9 homolog 1 (between residues 1-50). [LocusLink ID 54583]
Ordering Information
Pierce HIF Prolylhydroxylase 2 Antibody
Product #
PA1-16524
Size
100 µl
Price
$330.00
Purchase
Add HIF Prolylhydroxylase 2 Antibody to your cart.
Tested Applications Dilution *
Western Blot (WB) 1:500-1:2500
Immunofluorescence (IF) 1:50
Immunocytochemistry (ICC) 1:50
Immunohistochemistry (Paraffin) (IHC (P)) 1:10-1:500
Flow Cytometry (FACS) 3 µg/ml
Immunoprecipitation (IP) Assay-Dependent
* Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own experiment using appropriate negative and positive controls.
Form Information
Form: Liquid
Concentration: 1mg/ml
Purification: Antigen affinity chromatography
Storage Buffer: citric acid, pH 7-8
Preservative: 0.09% sodium azide
Storage Conditions: 4° C, do not freeze
Product Specific Information
This antibody does not react with mouse samples.

Suggested positive control: antigen standard for EGLN1 (transient overexpression lysate).
General Information
HIF prolyl hydroxylase 2 is a prolyl hydroxylase that modifies HIF-alpha. Classic prolyl hydroxylases are found in the endoplasmic reticulum and modify collagen, whereas HIF is an intracellular protein and the HPH sites do not resemble those modifying collagen. HIF is a transcriptional complex that plays a critical role in oxygen homeostasis. HPH is an essential component of the pathway through which cells sense oxygen. In the presence of oxygen, HPHs convert specific prolyl residues in HIF-alpha to hydroxyproline, leading to HIF-alpha destruction. Low oxygen levels, sensed at the cellular level, cause the HIF conversion to be reduced so that HIF is stable and there is increased angiogenesis. HPH-2, specifically, catalyzes the posttranslational formation of 4-hydroxyproline in HIF alpha proteins. It hydroxylates HIF-1 alpha at Pro(402) and Pro(564), and HIF-2 alpha. It targets HIF through the hydroxylation for proteasomal degredation via the von Hippel-Lindau ubiquitylation complex.
Product Images
  • HIF Prolylhydroxylase 2 Antibody (PA1-16524) in WB
    PA1-16524_WesternBlot2.jpg

    Western Blot with anti-HIF Prolylhydroxylase 2 Polyclonal Antibody (PA1-16524) (PA1-16524_WesternBlot2.jpg)

    Western Blot with anti-HIF Prolylhydroxylase 2 Polyclonal Antibody (PA1-16524)

    Western Blot analysis of HeLa lysates using PA1-16524.
  • HIF Prolylhydroxylase 2 Antibody (PA1-16524) in WB
    PA1-16524_WesternBlot.jpg

    Western Blot with anti-HIF Prolylhydroxylase 2 Polyclonal Antibody (PA1-16524) (PA1-16524_WesternBlot.jpg)

    Western Blot with anti-HIF Prolylhydroxylase 2 Polyclonal Antibody (PA1-16524)

    Western Blot analysis of human PHD2, using PA1-16524. Samples: Recombinant FLAG-His-PHD1, PHD2 or PHD3 (10ng/lane) and whole cell lysate from HeLa cells.
  • HIF Prolylhydroxylase 2 Antibody (PA1-16524) in ICC
    PA1-16524_Immunoctyochemistry.jpg

    Immunocytochemistry with anti-HIF Prolylhydroxylase 2 Polyclonal Antibody (PA1-16524) (PA1-16524_Immunoctyochemistry.jpg)

    Immunocytochemistry with anti-HIF Prolylhydroxylase 2 Polyclonal Antibody (PA1-16524)

    Detection of HIF Prolyl Hydroxylase 2 (Green) in Hela cells using PA1-16524 at a dilution of 1:50. Nuclei (Blue) are counterstainde using Hoechst.
  • HIF Prolylhydroxylase 2 Antibody (PA1-16524) in IHC
    PA1-16524_Immunohistochemistry.jpg

    Immunohistochemistry with anti-HIF Prolylhydroxylase 2 Polyclonal Antibody (PA1-16524) (PA1-16524_Immunohistochemistry.jpg)

    Immunohistochemistry with anti-HIF Prolylhydroxylase 2 Polyclonal Antibody (PA1-16524)

    Immunohistochemical staining of Vascular Endothelium 40X in Lung using PA1-16524.
  • HIF Prolylhydroxylase 2 Antibody (PA1-16524) in FACS
    PA1-16524_FlowCytometry.jpg

    Flow Cytometry with anti-HIF Prolylhydroxylase 2 Polyclonal Antibody (PA1-16524) (PA1-16524_FlowCytometry.jpg)

    Flow Cytometry with anti-HIF Prolylhydroxylase 2 Polyclonal Antibody (PA1-16524)

    Flow cytometric detection of PHD2. 1 million Jurkat cells were fixed, permeabilized, and stained with 3.0 ug/ml anti-PHD2 PA1-16524 in a 150 ul reaction. Isotype control (black), anti-MLL1 (red).
(This product is for In Vitro experimental use only. Not for resale without express authorization.)
Part of Thermo Fisher Scientific