HIF-2 alpha Antibody

HIF-2 alpha Polyclonal Antibody for Western blot, IF, ICC, IHC (F), IHC (P), FACS, IP, ChIP, ELISA, GS

>> See 14 other antibodies for EPAS1 (Hypoxia inducible Factor 2 alpha, MOP2, Endothelial pas domain protein 1, Member of pas superfamily 2)
Synonyms:
Hypoxia inducible Factor 2 alpha, MOP2, Endothelial pas domain protein 1, Member of pas superfamily 2
Entrez Gene ID:
Details
Host / Isotype: Rabbit
Class: Polyclonal
Type: Antibody
Tested Species Reactivity: Human (Hu), Mouse (Ms), Rat (Rt), Fish (Fs), Non-human primate (Nhp), Ovine (Ov)
Immunogen: A peptide derived from the C-terminus of mouse/human HIF-2 alpha protein.
Ordering Information
Pierce HIF-2 alpha Antibody
Product #
PA1-16510
Size
100 µl
Price
$350.00
Purchase
Add HIF-2 alpha Antibody to your cart.
Tested Applications Dilution *
Western Blot (WB) 1 µg/ml-2 µg/ml
Immunofluorescence (IF) 1:100
Immunocytochemistry (ICC) 1:100
Immunohistochemistry (Frozen) (IHC (F)) Assay-Dependent
Immunohistochemistry (Paraffin) (IHC (P)) 1:100
Flow Cytometry (FACS) Assay-Dependent
Immunoprecipitation (IP) 5 µg/1mg lysate
ChIP assay (ChIP) 1:10-1:500
ELISA (ELISA) 1:100-1:2000
Gel Shift (GS) Assay-Dependent
* Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own experiment using appropriate negative and positive controls.
Form Information
Form: Liquid
Concentration: 1mg/ml
Purification: Antigen affinity chromatography
Storage Buffer: PBS
Preservative: 0.05% sodium azide
Storage Conditions: 4° C, do not freeze
Product Specific Information
Suggested positive control: Cos7 CoCl2-treated nuclear extract, PC12 nuclear extracts.
General Information
Hypoxia contributes significantly to the pathophysiology of major categories of human disease, including myocardial and cerebral ischemia, cancer, pulmonary hypertension, congenital heart disease and chronic obstructive pulmonary disease. HIF-2 alpha is predominantly expressed in highly vascularized tissues of adult humans and endothelial cells of the embryonic and adult mouse, whereas HIF-1alpha functions primarily in extravascular tissues. HIF-2 alpha is also a potent activator of the tie-2 gene, which is known to be selectively expressed in endothelial cells.
Product Images
  • HIF-2 alpha Antibody (PA1-16510) in WB
    PA1-16510_WesternBlot2.jpg

    Western Blot with anti-HIF-2 alpha Polyclonal Antibody (PA1-16510) (PA1-16510_WesternBlot2.jpg)

    Western Blot with anti-HIF-2 alpha Polyclonal Antibody (PA1-16510)

    Western Blot analysis on normoxic and hypoxic nuclear rat cell lysates using PA1-16510, anti-HIF-2 alpha.
  • HIF-2 alpha Antibody (PA1-16510) in WB
    PA1-16510_WesternBlot.jpg

    Western Blot with anti-HIF-2 alpha Polyclonal Antibody (PA1-16510) (PA1-16510_WesternBlot.jpg)

    Western Blot with anti-HIF-2 alpha Polyclonal Antibody (PA1-16510)

    Western blot analysis of HIF-2 alpha on Lane 1, Cobalt chloride treated COS7 nuclear extracts and Lane 2, Untreated COS7 nuclear extracts using PA1-16510.
  • HIF-2 alpha Antibody (PA1-16510) in ICC
    PA1-16510_Immunoctyochemistry.jpg

    Immunocytochemistry with anti-HIF-2 alpha Polyclonal Antibody (PA1-16510) (PA1-16510_Immunoctyochemistry.jpg)

    Immunocytochemistry with anti-HIF-2 alpha Polyclonal Antibody (PA1-16510)

    Detection of HIF-2 Alpha (Green) in RCC4 cells using PA1-16510. Nuclei (Blue) are counterstained with Hoechst 33258.
  • HIF-2 alpha Antibody (PA1-16510) in IHC
    PA1-16510_Immunohistochemistry.jpg

    Immunohistochemistry with anti-HIF-2 alpha Polyclonal Antibody (PA1-16510) (PA1-16510_Immunohistochemistry.jpg)

    Immunohistochemistry with anti-HIF-2 alpha Polyclonal Antibody (PA1-16510)

    Hif-2 alpha immunoreactivity in human cardiac myocytes stained with PA1-16510.
(This product is for In Vitro experimental use only. Not for resale without express authorization.)
Part of Thermo Fisher Scientific