* Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own experiment using appropriate negative and positive controls.
Antigen affinity chromatography
0.05% sodium azide
4° C, do not freeze
Product Specific Information
PA1-16510 reacts with HIF-2 alpha in mouse, rat, fish, non-human primate, and human samples.
PA1-16510 has been successfully used in Western Blot, Chromatin Immunoprecipitation, ELISA, Flow Cytometry, Immunocytochemistry/Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin and Immunoprecipitation applications.
The PA1-16510 immunogen is a peptide derived from the C-terminus of mouse/human HIF-2 alpha protein.
Hypoxia contributes significantly to the pathophysiology of major categories of human disease, including myocardial and cerebral ischemia, cancer, pulmonary hypertension, congenital heart disease and chronic obstructive pulmonary disease. HIF-2 alpha is predominantly expressed in highly vascularized tissues of adult humans and endothelial cells of the embryonic and adult mouse, whereas HIF-1alpha functions primarily in extravascular tissues. HIF-2 alpha is also a potent activator of the tie-2 gene, which is known to be selectively expressed in endothelial cells.
HIF-2 alpha Antibody (PA1-16510) in WB EPAS1_Antibody_PA1-16510_WesternBlot(WB).jpg
Western Blot with anti-HIF-2 alpha Polyclonal Antibody (PA1-16510)