HIF-1 alpha Antibody (mgc3)

HIF-1 alpha Monoclonal Antibody for Western blot, IF, ICC, IHC, IHC (P), IP, GS, IE

>> See 28 other antibodies for HIF1A (Hypoxia Inducible Factor 1 alpha, ARNT interacting protein, ARNT2, MOP1, PASD8)
Synonyms:
Hypoxia Inducible Factor 1 alpha, ARNT interacting protein, ARNT2, MOP1, PASD8
Details
Host / Isotype: Mouse / IgG1
Class: Monoclonal
Type: Antibody
Clone: mgc3
Tested Species Reactivity: Human (Hu), Mouse (Ms), Bovine (Bv), Non-human primate (Nhp), Porcine (Po)
Published Species Reactivity: Bovine (Bv), Human (Hu), Mouse (Ms), Non-human primate (Nhp), Rabbit (Rb), Rat (Rt)
Immunogen: Human HIF-1 alpha amino acids 530-826.
Ordering Information
Pierce HIF-1 alpha Antibody (mgc3)
Product #
MA1-516
Size
100 µl
Price
$360.00
Purchase
Add HIF-1 alpha Antibody (mgc3) to your cart.
Tested Applications Dilution *
Western Blot (WB) 1:2,000
Immunofluorescence (IF) 1:200
Immunocytochemistry (ICC) Assay dependent
Immunohistochemistry (IHC) 1:20
Immunohistochemistry (Paraffin) (IHC (P)) 1/800
Immunoprecipitation (IP) Assay dependent
Gel Shift (GS) Assay dependent
Immunoelectrophoresis (IE) Assay Dependent
Published Applications Dilution
Western Blot (WB) See publications below
Immunocytochemistry (ICC) See publications below
Immunohistochemistry (IHC) See publications below
Immunoprecipitation (IP) See publications below
Gel Shift (GS) See publications below
Blocking Assay (BLOCK) See publications below
* Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own experiment using appropriate negative and positive controls.
Form Information
Form: Liquid
Concentration: 1mg/ml
Purification: purified
Storage Buffer: 1mg/ml BSA
Preservative: 0.05% sodium azide
Storage Conditions: -20° C, Avoid Freeze/Thaw Cycles
Product Specific Information
MA1-516 detects hypoxia-inducible factor 1 alpha (HIF-1 alpha) from human, non-human primate, bovine, mouse and porcine cells. This antibody does not cross-react with ARNT or the related HIF-2 alpha.

MA1-516 has been successfully used in Western blot, immunofluorescence, immunoprecipitation, immunocytochemistry, and gel shift procedures. By Western blot, this antibody detects an ~116 kDa protein representing HIF-1 alpha after hypoxic induction in COS cells. Immunofluorescence staining of HIF-1 alpha in COS-7 cells with MA1-516 yields nuclear staining after exposing cells to 1% oxygen for 4 hours. In gel shift assays, MA1-516 has been successfully used only with mouse HIF-1 alpha.

MA1-516 antigen is a peptide corresponding to amino acids 530-826 of human HIF-1 alpha.



Antibodies to this protein (and modification) were previously sold as part of a Thermo Scientific Cellomics High Content Screening Kit. This replacement antibody is now recommended for researchers who need an antibody for high content cell based assays. It has been thoroughly tested and validated for cellular immunofluorescence (IF) applications. Further optimization including the selection of the most appropriate fluorescent Dylight conjugated secondary antibody may have to be performed for your high content assay.
General Information
Oxygen availability plays a major role in the regulation of expression of many different genes including erythropoietin, nitric oxide synthase (NOS), heme oxygenase 1 (HO-1), glucose transporters and vascular growth factors necessary for the maintenance of homeostasis in hypoxic conditions. Hypoxia-inducible factor 1 alpha (HIF-1 alpha) has been identified as a bHLH-PAS family member which is instrumental in the oxygen-dependent regulation of these genes. HIF-1 alpha rapidly accumulates in the nucleus upon exposure to hypoxic conditions where it heterodimerizes with the aryl hydrocarbon nuclear receptor translocator, ARNT, also referred to as HIF-1 beta.
Product Images
  • HIF-1 alpha Antibody (MA1-516) in WB
    HIF1A-Antibody-MA1-516-WesternBlot-WB.jpg

    Western Blot with anti-HIF-1 alpha Monoclonal Antibody [mgc3] (MA1-516) (HIF1A-Antibody-MA1-516-WesternBlot-WB.jpg)

    Western Blot with anti-HIF-1 alpha Monoclonal Antibody [mgc3] (MA1-516)

    Western blot of HIF-1 alpha in HeLa cells using MA1-516.
  • HIF-1 alpha Antibody (MA1-516) in IF
    HIF1A-Antibody-MA1-516-Immunofluorescence-IF.jpg

    Immunofluorescence with anti-HIF-1 alpha Monoclonal Antibody [mgc3] (MA1-516) (HIF1A-Antibody-MA1-516-Immunofluorescence-IF.jpg)

    Immunofluorescence with anti-HIF-1 alpha Monoclonal Antibody [mgc3] (MA1-516)

    Immunofluorescence staining of HIF-1 Alpha using MA1-516.
  • HIF-1 alpha Antibody (MA1-516) in IF
    HIF-1-alpha_MA1-516_Immunofluorescence_A2058_Cells.jpg

    Immunofluorescence with anti-HIF-1 alpha Monoclonal Antibody [mgc3] (MA1-516) (HIF-1-alpha_MA1-516_Immunofluorescence_A2058_Cells.jpg)

    Immunofluorescence with anti-HIF-1 alpha Monoclonal Antibody [mgc3] (MA1-516)

    Immunofluorescent analysis of HIF-1 alpha using HIF-1 alpha Monoclonal Antibody (mgc3) (Product# MA1-516 ) shows staining in A2058 Cells. HIF-1 alpha (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with an antibody recognizing HIF-1 alpha (Product# MA1-516 ) at a dilution of 1:20 over night at 4 ?C, washed with PBS and incubated with a DyLight-488 conjugated secondary antibody (Product# 35552 for GAR, Product# 35503 for GAM). Images were taken at 60X magnification.
  • HIF-1 alpha Antibody (MA1-516) in IF
    HIF-1-alpha_MA1-516_Immunofluorescence_Hela_Cells.jpg

    Immunofluorescence with anti-HIF-1 alpha Monoclonal Antibody [mgc3] (MA1-516) (HIF-1-alpha_MA1-516_Immunofluorescence_Hela_Cells.jpg)

    Immunofluorescence with anti-HIF-1 alpha Monoclonal Antibody [mgc3] (MA1-516)

    Immunofluorescent analysis of HIF-1 alpha using HIF-1 alpha Monoclonal Antibody (mgc3) (Product# MA1-516 ) shows staining in Hela Cells. HIF-1 alpha (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with an antibody recognizing HIF-1 alpha (Product# MA1-516 ) at a dilution of 1:20 over night at 4 ?C, washed with PBS and incubated with a DyLight-488 conjugated secondary antibody (Product# 35552 for GAR, Product# 35503 for GAM). Images were taken at 60X magnification.
  • HIF-1 alpha Antibody (MA1-516) in IF
    HIF-1-alpha_MA1-516_Immunofluorescence_U251_Cells.jpg

    Immunofluorescence with anti-HIF-1 alpha Monoclonal Antibody [mgc3] (MA1-516) (HIF-1-alpha_MA1-516_Immunofluorescence_U251_Cells.jpg)

    Immunofluorescence with anti-HIF-1 alpha Monoclonal Antibody [mgc3] (MA1-516)

    Immunofluorescent analysis of HIF-1 alpha using HIF-1 alpha Monoclonal Antibody (mgc3) (Product# MA1-516 ) shows staining in U251 Cells. HIF-1 alpha (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with an antibody recognizing HIF-1 alpha (Product# MA1-516 ) at a dilution of 1:20 over night at 4 ?C, washed with PBS and incubated with a DyLight-488 conjugated secondary antibody (Product# 35552 for GAR, Product# 35503 for GAM). Images were taken at 60X magnification.
  • HIF-1 alpha Antibody (MA1-516) in IF
    HIF-1-alpha_Antibody_MA1-516_Immunofluorescence_5_20130717153435.jpg

    Immunofluorescence with anti-HIF-1 alpha Monoclonal Antibody [mgc3] (MA1-516) (HIF-1-alpha_Antibody_MA1-516_Immunofluorescence_5_20130717153435.jpg)

    Immunofluorescence with anti-HIF-1 alpha Monoclonal Antibody [mgc3] (MA1-516)

    Immunofluorescent analysis of HIF-1 alpha (green) in HeLa cells either left untreated (left panel) or treated with 100uM Deferoxamine mesylate for ~16 hours (right panel). Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 15 minutes at room temperature and blocked with 0.3% BSA for 15 minutes at room temperature. Cells were probed with a HIF-1 alpha monoclonal antibody (Product # MA1-516) at a dilution of 1:100 for at least 1 hour at room temperature, washed with PBS, and incubated with a DyLight 488-conjugated goat anti-mouse IgG secondary antibody (Product # 35502) at a dilution of 1:500 for 30 minutes at room temperature. F-actin (red) was stained with DyLight 594 Phalloidin (Product # 21836) and nuclei (blue) were stained with Hoechst 33342 dye (Product # 62249). Images were taken on a Thermo Scientific ArrayScan Instrument at 20X magnification.
  • HIF-1 alpha Antibody (MA1-516) in IHC
    MA1-516_IHC_Tonsil_tissue.jpg

    Immunohistochemistry with anti-HIF-1 alpha Monoclonal Antibody [mgc3] (MA1-516) (MA1-516_IHC_Tonsil_tissue.jpg)

    Immunohistochemistry with anti-HIF-1 alpha Monoclonal Antibody [mgc3] (MA1-516)

    Immunohistochemistry was performed on normal biopsies of deparaffinized Human tonsil tissue. To expose target proteins, heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes. Following antigen retrieval tissues were blocked in 3% BSA-PBS for 30 minutes at room temperature. Tissues were then probed at a dilution of 1:20 with a mouse monoclonal antibody recognizing HIF-1 alpha (MA1-516) or without primary antibody (negative control) overnight at 4°C in a humidified chamber. Tissues were washed extensively with PBST and endogenous peroxidase activity was quenched with a peroxidase suppressor. Detection was performed using a biotin-conjugated secondary antibody and SA-HRP, followed by colorimetric detection using DAB. Tissues were counterstained with hematoxylin and prepped for mounting.
Publications:
Western Blot
  Species / Dilution Summary
  Bv / 0
MA1-516 was used in western blot to investigate the influence of hypoxia on corneal stromal cell survival

Exp Eye Res. 2006 May;82(5):780-7.
"Hypoxia preconditioning protects corneal stromal cells against induced apoptosis."
Author(s): Xing D, Sun X, Li J, Cui M, Tan-Allen K, Bonanno JA
Number of Citations: 1
(See PubMed article )
  Bv / 1:2,000
MA1-516 was used in western blot to investigate the role of HIF-1alpha on hypoxia preconditioning protection in corneal stromal cells

Mol Vis. 2009 May 18;15():1020-7.
"Hypoxia preconditioning protection of corneal stromal cells requires HIF1alpha but not VEGF."
Author(s): Xing D, Bonanno JA
Number of Citations: 1
(See PubMed article )
  Hu / Not Cited
MA1-516 was used in immunocytochemistry, EMSA assay, immunoprecipitation and western blot to test antibodies isolated from chicken egg yolk

FASEB J. 1999 Jan;13(1):81-8.
"General applicability of chicken egg yolk antibodies: the performance of IgY immunoglobulins raised against the hypoxia-inducible factor 1alpha."
Author(s): Camenisch G, Tini M, Chilov D, Kvietikova I, Srinivas V, Caro J, Spielmann P, Wenger RH, Gassmann M
Number of Citations: 11
(See PubMed article )
  Hu / Not Cited
MA1-516 was used in western blot, immunocytochemistry and EMSA to study the effect of epolones on erythropoietin expression.

Blood. 2000 Aug 15;96(4):1558-65.
"Epolones induce erythropoietin expression via hypoxia-inducible factor-1 alpha activation."
Author(s): Wanner RM, Spielmann P, Stroka DM, Camenisch G, Camenisch I, Scheid A, Houck DR, Bauer C, Gassmann M, Wenger RH
Number of Citations: 7
(See PubMed article )
  Hu / 1:2,000
MA1-516 was used in western blot to investigate the role of bcl-2 in tumorigenesis

J Natl Cancer Inst. 2001 Feb 7;93(3):208-13.
"Angiogenic potential of prostate carcinoma cells overexpressing bcl-2."
Author(s): Fernandez A, Udagawa T, Schwesinger C, Beecken W, Achilles-Gerte E, McDonnell T, D'Amato R
Number of Citations: 1
(See PubMed article )
  Hu / 1:250
MA1-516 was used in immunohistochemistry, immunoprecipitation and western blot to study how HIF1A is regulated during the development of human placenta

Biol Reprod. 2006 Jul;75(1):112-21.
"Dynamic HIF1A regulation during human placental development."
Author(s): Ietta F, Wu Y, Winter J, Xu J, Wang J, Post M, Caniggia I
Number of Citations: 1
(See PubMed article )
  Hu / Not Cited
MA1-516 was used in western blot and immunohistochemistry to study the hypoxia-inducible factor-1alpha expression in human placenta.

Am J Pathol. 2007 Jun;170(6):2171-9.
"Human placental hypoxia-inducible factor-1alpha expression correlates with clinical outcomes in chronic hypoxia in vivo."
Author(s): Zamudio S, Wu Y, Ietta F, Rolfo A, Cross A, Wheeler T, Post M, Illsley NP, Caniggia I
Number of Citations: 5
(See PubMed article )
  Hu / 0
MA1-516 was used in western blot to study the upregulation of carcinoembryonic antigen expression in cancer cells by hypoxia

Int J Cancer. 2007 Dec 1;121(11):2443-50.
"Hypoxia upregulates carcinoembryonic antigen expression in cancer cells."
Author(s): Kokkonen N, Ulibarri IF, Kauppila A, Luosujärvi H, Rivinoja A, Pospiech H, Kellokumpu I, Kellokumpu S
Number of Citations: 3
(See PubMed article )
  Hu / 1:2000
MA1-516 was used in immunocytochemistry and western blot to investigate the effect of hypoxia on regulating the transcriptional activity of androgen receptor

J Steroid Biochem Mol Biol. 2011 Jan;123(1-2):58-64.
"Hypoxia enhances transcriptional activity of androgen receptor through hypoxia-inducible factor-1? in a low androgen environment."
Author(s): Mitani T, Yamaji R, Higashimura Y, Harada N, Nakano Y, Inui H
Number of Citations: 1
(See PubMed article )
  Hu / 0
MA1-516 was used in western blot to investigate the effect of HIF-1 on the regulation of GAPDH expression in hypoxic breast cancer cells

Arch Biochem Biophys. 2011 May 1;509(1):1-8.
"Up-regulation of glyceraldehyde-3-phosphate dehydrogenase gene expression by HIF-1 activity depending on Sp1 in hypoxic breast cancer cells."
Author(s): Higashimura Y, Nakajima Y, Yamaji R, Harada N, Shibasaki F, Nakano Y, Inui H
Number of Citations: 1
(See PubMed article )
  Hu / 1:2000
MA1-516 was used in immunohistochemistry and western blot to study the role of HIF-1alpha in the upregulation of HIF-1beta in hypoxic human melanoma cells

Biochem Biophys Res Commun. 2013 Apr 26;434(1):166-72.
"Hypoxia-inducible factor-1? (HIF-1?) is upregulated in a HIF-1?-dependent manner in 518A2 human melanoma cells under hypoxic conditions."
Author(s): Mandl M, Kapeller B, Lieber R, Macfelda K
Number of Citations: 1
(See PubMed article )
  Ms / Not Cited
MA1-516 was used in immunocytochemistry, EMSA assay, immunoprecipitation and western blot to test antibodies isolated from chicken egg yolk

FASEB J. 1999 Jan;13(1):81-8.
"General applicability of chicken egg yolk antibodies: the performance of IgY immunoglobulins raised against the hypoxia-inducible factor 1alpha."
Author(s): Camenisch G, Tini M, Chilov D, Kvietikova I, Srinivas V, Caro J, Spielmann P, Wenger RH, Gassmann M
Number of Citations: 11
(See PubMed article )
  Ms / 1:100
MA1-516 was used in western blot to study the effect of oxygen level during mouse lung development

Am J Physiol Lung Cell Mol Physiol. 2005 Jan;288(1):L167-78.
"Role of oxygen and vascular development in epithelial branching morphogenesis of the developing mouse lung."
Author(s): van Tuyl M, Liu J, Wang J, Kuliszewski M, Tibboel D, Post M
Number of Citations: 1
(See PubMed article )
  Ms / 1:500
MA1-516 was used in western blot to study the role of galectin-1 interactions with N-glycans in hypoxia-mediated angiogenesis and tumorigenesis in Kaposi's sarcoma

J Exp Med. 2012 Oct 22;209(11):1985-2000.
"Disrupting galectin-1 interactions with N-glycans suppresses hypoxia-driven angiogenesis and tumorigenesis in Kaposi's sarcoma."
Author(s): Croci DO, Salatino M, Rubinstein N, Cerliani JP, Cavallin LE, Leung HJ, Ouyang J, Ilarregui JM, Toscano MA, Domaica CI, Croci MC, Shipp MA, Mesri EA, Albini A, Rabinovich GA
Number of Citations: 7
(See PubMed article )
  Rt / 1:1500
MA1-516 was used in western blot to study the role of HIF-1alpha and VEGF in the neuroprotective effects of soy following acute stroke

Endocrinology. 2013 Apr;154(4):1589-97.
"Hypoxia-inducible factor and vascular endothelial growth factor are targets of dietary soy during acute stroke in female rats."
Author(s): Ma Y, Lovekamp-Swan T, Bekele W, Dohi A, Schreihofer DA
Number of Citations: 1
(See PubMed article )
Immunocytochemistry
  Species / Dilution Summary
  Hu / 1:10
MA1-516 was used in western blot, immunocytochemistry and EMSA to study the effect of epolones on erythropoietin expression.

Blood. 2000 Aug 15;96(4):1558-65.
"Epolones induce erythropoietin expression via hypoxia-inducible factor-1 alpha activation."
Author(s): Wanner RM, Spielmann P, Stroka DM, Camenisch G, Camenisch I, Scheid A, Houck DR, Bauer C, Gassmann M, Wenger RH
Number of Citations: 7
(See PubMed article )
  Hu / 1:500
MA1-516 was used in immunocytochemistry and western blot to investigate the effect of hypoxia on regulating the transcriptional activity of androgen receptor

J Steroid Biochem Mol Biol. 2011 Jan;123(1-2):58-64.
"Hypoxia enhances transcriptional activity of androgen receptor through hypoxia-inducible factor-1? in a low androgen environment."
Author(s): Mitani T, Yamaji R, Higashimura Y, Harada N, Nakano Y, Inui H
Number of Citations: 1
(See PubMed article )
  Nhp / Not Cited
MA1-516 was used in immunocytochemistry, EMSA assay, immunoprecipitation and western blot to test antibodies isolated from chicken egg yolk

FASEB J. 1999 Jan;13(1):81-8.
"General applicability of chicken egg yolk antibodies: the performance of IgY immunoglobulins raised against the hypoxia-inducible factor 1alpha."
Author(s): Camenisch G, Tini M, Chilov D, Kvietikova I, Srinivas V, Caro J, Spielmann P, Wenger RH, Gassmann M
Number of Citations: 11
(See PubMed article )
Immunohistochemistry
  Species / Dilution Summary
  Hu / 1:50
MA1-516 was used in immunohistochemistry, immunoprecipitation and western blot to study how HIF1A is regulated during the development of human placenta

Biol Reprod. 2006 Jul;75(1):112-21.
"Dynamic HIF1A regulation during human placental development."
Author(s): Ietta F, Wu Y, Winter J, Xu J, Wang J, Post M, Caniggia I
Number of Citations: 1
(See PubMed article )
  Hu / 1:50
MA1-516 was used in western blot and immunohistochemistry to study the hypoxia-inducible factor-1alpha expression in human placenta.

Am J Pathol. 2007 Jun;170(6):2171-9.
"Human placental hypoxia-inducible factor-1alpha expression correlates with clinical outcomes in chronic hypoxia in vivo."
Author(s): Zamudio S, Wu Y, Ietta F, Rolfo A, Cross A, Wheeler T, Post M, Illsley NP, Caniggia I
Number of Citations: 5
(See PubMed article )
  Hu / 1:50
MA1-516 was used in immunohistochemistry and western blot to study the role of HIF-1alpha in the upregulation of HIF-1beta in hypoxic human melanoma cells

Biochem Biophys Res Commun. 2013 Apr 26;434(1):166-72.
"Hypoxia-inducible factor-1? (HIF-1?) is upregulated in a HIF-1?-dependent manner in 518A2 human melanoma cells under hypoxic conditions."
Author(s): Mandl M, Kapeller B, Lieber R, Macfelda K
Number of Citations: 1
(See PubMed article )
  Rb / 1:40
MA1-516 was used in immunohistochemistry to study the development of arterial vasa vasorum in a rabbit model

Cell Tissue Res. 2002 Mar;307(3):303-13.
"Experimental angiogenesis of arterial vasa vasorum."
Author(s): Bayer IM, Caniggia I, Adamson SL, Langille BL
Number of Citations: 4
(See PubMed article )
Immunoprecipitation
  Species / Dilution Summary
  Hu / Not Cited
MA1-516 was used in immunocytochemistry, EMSA assay, immunoprecipitation and western blot to test antibodies isolated from chicken egg yolk

FASEB J. 1999 Jan;13(1):81-8.
"General applicability of chicken egg yolk antibodies: the performance of IgY immunoglobulins raised against the hypoxia-inducible factor 1alpha."
Author(s): Camenisch G, Tini M, Chilov D, Kvietikova I, Srinivas V, Caro J, Spielmann P, Wenger RH, Gassmann M
Number of Citations: 11
(See PubMed article )
  Hu / Not Cited
MA1-516 was used in immunohistochemistry, immunoprecipitation and western blot to study how HIF1A is regulated during the development of human placenta

Biol Reprod. 2006 Jul;75(1):112-21.
"Dynamic HIF1A regulation during human placental development."
Author(s): Ietta F, Wu Y, Winter J, Xu J, Wang J, Post M, Caniggia I
Number of Citations: 1
(See PubMed article )
  Ms / Not Cited
MA1-516 was used in immunocytochemistry, EMSA assay, immunoprecipitation and western blot to test antibodies isolated from chicken egg yolk

FASEB J. 1999 Jan;13(1):81-8.
"General applicability of chicken egg yolk antibodies: the performance of IgY immunoglobulins raised against the hypoxia-inducible factor 1alpha."
Author(s): Camenisch G, Tini M, Chilov D, Kvietikova I, Srinivas V, Caro J, Spielmann P, Wenger RH, Gassmann M
Number of Citations: 11
(See PubMed article )
Gel Shift
  Species / Dilution Summary
  Hu / Not Cited
MA1-516 was used in EMSA assay to investigate the regulation of HIF-1 DNA binding and transcriptional activation by CpG methylation

Eur J Biochem. 1998 May 1;253(3):771-7.
"Oxygen-regulated erythropoietin gene expression is dependent on a CpG methylation-free hypoxia-inducible factor-1 DNA-binding site."
Author(s): Wenger RH, Kvietikova I, Rolfs A, Camenisch G, Gassmann M
Number of Citations: 8
(See PubMed article )
  Hu / Not Cited
MA1-516 was used in immunocytochemistry, EMSA assay, immunoprecipitation and western blot to test antibodies isolated from chicken egg yolk

FASEB J. 1999 Jan;13(1):81-8.
"General applicability of chicken egg yolk antibodies: the performance of IgY immunoglobulins raised against the hypoxia-inducible factor 1alpha."
Author(s): Camenisch G, Tini M, Chilov D, Kvietikova I, Srinivas V, Caro J, Spielmann P, Wenger RH, Gassmann M
Number of Citations: 11
(See PubMed article )
  Hu / Not Cited
MA1-516 was used in western blot, immunocytochemistry and EMSA to study the effect of epolones on erythropoietin expression.

Blood. 2000 Aug 15;96(4):1558-65.
"Epolones induce erythropoietin expression via hypoxia-inducible factor-1 alpha activation."
Author(s): Wanner RM, Spielmann P, Stroka DM, Camenisch G, Camenisch I, Scheid A, Houck DR, Bauer C, Gassmann M, Wenger RH
Number of Citations: 7
(See PubMed article )
  Ms / Not Cited
MA1-516 was used in EMSA assay to investigate the regulation of HIF-1 DNA binding and transcriptional activation by CpG methylation

Eur J Biochem. 1998 May 1;253(3):771-7.
"Oxygen-regulated erythropoietin gene expression is dependent on a CpG methylation-free hypoxia-inducible factor-1 DNA-binding site."
Author(s): Wenger RH, Kvietikova I, Rolfs A, Camenisch G, Gassmann M
Number of Citations: 8
(See PubMed article )
  Ms / Not Cited
MA1-516 was used in immunocytochemistry, EMSA assay, immunoprecipitation and western blot to test antibodies isolated from chicken egg yolk

FASEB J. 1999 Jan;13(1):81-8.
"General applicability of chicken egg yolk antibodies: the performance of IgY immunoglobulins raised against the hypoxia-inducible factor 1alpha."
Author(s): Camenisch G, Tini M, Chilov D, Kvietikova I, Srinivas V, Caro J, Spielmann P, Wenger RH, Gassmann M
Number of Citations: 11
(See PubMed article )
Blocking Assay
  Species / Dilution Summary
  Ms / Not Cited
MA1-516 was used in blocking or activating experiment to study the role of the soluble isoform of FLT-1 in modulating podocyte/pericyte function

Cell. 2012 Oct 12;151(2):384-99.
"Soluble FLT1 binds lipid microdomains in podocytes to control cell morphology and glomerular barrier function."
Author(s): Jin J, Sison K, Li C, Tian R, Wnuk M, Sung HK, Jeansson M, Zhang C, Tucholska M, Jones N, Kerjaschki D, Shibuya M, Fantus IG, Nagy A, Gerber HP, Ferrara N, Pawson T, Quaggin SE
Number of Citations: 7
(See PubMed article )
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