HCN4 Antibody (SHG 1E5)

HCN4 Monoclonal Antibody for Western blot, IF, IHC, IHC (F), IHC (P), FACS

>> See 2 other antibodies for HCN4 (Hyperpolarization-Activated Cyclic Nucleotide-Gated Potassium Channel-4)
Synonyms:
Hyperpolarization-Activated Cyclic Nucleotide-Gated Potassium Channel-4
Entrez Gene ID:
Details
Host / Isotype: Rat / IgG1
Class: Monoclonal
Type: Antibody
Clone: SHG 1E5
Tested Species Reactivity: Human (Hu), Mouse (Ms), Rat (Rt)
Published Species Reactivity: Mouse (Ms), Rat (Rt)
Immunogen: Synthetic peptide sequence corresponding to residues S H G S L L L P P A S S P P P P Q V P Q R R G T P P L T P G R L T Q D L K L of HCN4.
Ordering Information
Pierce HCN4 Antibody (SHG 1E5)
Product #
MA3-903
Size
100 µl
Price
$360.00
Purchase
Add HCN4 Antibody (SHG 1E5) to your cart.
Tested Applications Dilution *
Western Blot (WB) 1:5000
Immunofluorescence (IF) Assay Dependent
Immunohistochemistry (IHC) 1:1000
Immunohistochemistry (Frozen) (IHC (F)) 1/1000
Immunohistochemistry (Paraffin) (IHC (P)) Assay Dependent
Flow Cytometry (FACS) 1/50
Published Applications Dilution
Western Blot (WB) See publications below
Immunocytochemistry (ICC) See publications below
Immunohistochemistry (IHC) See publications below
* Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own experiment using appropriate negative and positive controls.
Form Information
Form: Liquid
Concentration: 1mg/ml
Storage Buffer: ascites
Preservative: 0.05% sodium azide
Storage Conditions: -20° C, Avoid Freeze/Thaw Cycles
Product Specific Information
MA3-903 detects HCN4 from human, mouse, and rat samples.

MA3-903 has been successfully used in Western blotting and immunohistochemistry procedures. By Western blot MA3-903 detects a ~132 KDa band representing HCN4.

The MA3-903 immunogen is a synthetic peptide sequence corresponding to residues S H G S L L L P P A S S P P P P Q V P Q R R G T P P L T P G R L T Q D L K L of HCN4.

MA3-903 clone SHG 1E5 is a rat monoclonal hybridoma. This antibody was produced by immunizing a rat, isolating the spleen cells, creating the hybridoma and successively injecting these cells into a mouse to produce ascites.
General Information
HCN4 is a member of the family of hyperpolarization-activated and cyclic-nucleotide-gated (HCN) channels. HCN currents have been linked to pacemaker activity in the heart and brain, resting potential control, as well as neuronal plasticity. It has been shown that HCN4 channels function as receptors for sour taste, and are associated with pacemaker potential generation in the sinoatrial node.
Product Images
  • HCN4 Antibody (MA3-903) in IHC
    MA3-903_Immunohistochemistry_Heart tissue.jpg

    Immunohistochemistry with anti-HCN4 Monoclonal Antibody [SHG 1E5] (MA3-903) (MA3-903_Immunohistochemistry_Heart tissue.jpg)

    Immunohistochemistry with anti-HCN4 Monoclonal Antibody [SHG 1E5] (MA3-903)

    Immunohistochemistry was performed on normal deparaffinized Human heart tissue tissues. To expose target proteins, heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes. Following antigen retrieval tissues were blocked in 3% BSA-PBS for 30 minutes at room temperature. Tissues were then probed at a dilution of 1:20 with a rat monoclonal antibody recognizing HCN4 (MA3-903) or without primary antibody (negative control) overnight at 4°C in a humidified chamber. Tissues were washed extensively with PBST and endogenous peroxidase activity was quenched with a peroxidase suppressor. Detection was performed using a biotin-conjugated secondary antibody and SA-HRP, followed by colorimetric detection using DAB. Tissues were counterstained with hematoxylin and prepped for mounting.
  • HCN4 Antibody (MA3-903) in IHC
    MA3-903_Immunohistochemistry_Tonsil tissue.jpg

    Immunohistochemistry with anti-HCN4 Monoclonal Antibody [SHG 1E5] (MA3-903) (MA3-903_Immunohistochemistry_Tonsil tissue.jpg)

    Immunohistochemistry with anti-HCN4 Monoclonal Antibody [SHG 1E5] (MA3-903)

    Immunohistochemistry was performed on normal deparaffinized Human tonsil tissue tissues. To expose target proteins, heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes. Following antigen retrieval tissues were blocked in 3% BSA-PBS for 30 minutes at room temperature. Tissues were then probed at a dilution of 1:20 with a rat monoclonal antibody recognizing HCN4 (MA3-903) or without primary antibody (negative control) overnight at 4°C in a humidified chamber. Tissues were washed extensively with PBST and endogenous peroxidase activity was quenched with a peroxidase suppressor. Detection was performed using a biotin-conjugated secondary antibody and SA-HRP, followed by colorimetric detection using DAB. Tissues were counterstained with hematoxylin and prepped for mounting.
Publications:
Western Blot
  Species / Dilution Summary
  Ms / Not Cited
Rt / Not Cited
MA3-903 was used in immunocytochemistry, immunohistochemistry and western blot to investigate cyclic-nucleotide-gated channels as receptors for sour taste

Nature. 2001 Oct 11;413(6856):631-5.
"Hyperpolarization-activated channels HCN1 and HCN4 mediate responses to sour stimuli."
Author(s): Stevens DR, Seifert R, Bufe B, Müller F, Kremmer E, Gauss R, Meyerhof W, Kaupp UB, Lindemann B
Number of Citations: 1
(See PubMed article )
  Rt / Not Cited
MA3-903 was used in immunohistochemistry and western blot to study the expression of HCN channels in retinal bipolar cells

Eur J Neurosci. 2003 May;17(10):2084-96.
"HCN channels are expressed differentially in retinal bipolar cells and concentrated at synaptic terminals."
Author(s): Müller F, Scholten A, Ivanova E, Haverkamp S, Kremmer E, Kaupp UB
Number of Citations: 9
(See PubMed article )
Immunocytochemistry
  Species / Dilution Summary
  Ms / Not Cited
Rt / Not Cited
MA3-903 was used in immunocytochemistry, immunohistochemistry and western blot to investigate cyclic-nucleotide-gated channels as receptors for sour taste

Nature. 2001 Oct 11;413(6856):631-5.
"Hyperpolarization-activated channels HCN1 and HCN4 mediate responses to sour stimuli."
Author(s): Stevens DR, Seifert R, Bufe B, Müller F, Kremmer E, Gauss R, Meyerhof W, Kaupp UB, Lindemann B
Number of Citations: 1
(See PubMed article )
Immunohistochemistry
  Species / Dilution Summary
  Ms / 1:5
Rt / 1:5
MA3-903 was used in immunocytochemistry, immunohistochemistry and western blot to investigate cyclic-nucleotide-gated channels as receptors for sour taste

Nature. 2001 Oct 11;413(6856):631-5.
"Hyperpolarization-activated channels HCN1 and HCN4 mediate responses to sour stimuli."
Author(s): Stevens DR, Seifert R, Bufe B, Müller F, Kremmer E, Gauss R, Meyerhof W, Kaupp UB, Lindemann B
Number of Citations: 1
(See PubMed article )
  Rt / Not Cited
MA3-903 was used in immunohistochemistry and western blot to study the expression of HCN channels in retinal bipolar cells

Eur J Neurosci. 2003 May;17(10):2084-96.
"HCN channels are expressed differentially in retinal bipolar cells and concentrated at synaptic terminals."
Author(s): Müller F, Scholten A, Ivanova E, Haverkamp S, Kremmer E, Kaupp UB
Number of Citations: 9
(See PubMed article )
(This product is for In Vitro experimental use only. Not for resale without express authorization.)
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