| Details | |
| Host / Isotype: | Goat |
| Class: | Polyclonal |
| Type: | Secondary Antibody |
| Label: | Unconjugated |
| Species Reactivity: | Chicken (Ck) |
| Immunogen: | Chicken IgY whole molecule |
| Ordering Information | ||||
| Pierce Goat anti-Chicken IgY Secondary Antibody |
| Storage: | 4° C, do not freeze |
| Form: | 1 mg of sterile-filtered antibody in 0.15M NaCl, 0.05M Tris Chloride, 0.001M Mg Chloride, 0.0001M Zn Chloride, 50% (v/v) glycerol, pH 8.0, with 10 mg/ml BSA and 0.01% sodium azide. |
| Applications | Dilution * |
| Immunohistochemistry (Frozen) (IHC (F)) | Assay dependent |
| Immunoprecipitation (IP) | Assay dependent |
| Immunodiffusion (IDF) | Assay-dependent |
| Immunohistochemistry (Paraffin) (IHC (P)) | Assay dependent |
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* Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own experiment using appropriate negative and positive controls.
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| Product Specific Information |
| PA1-28653 detects Chicken IgY from chicken samples. PA1-28653 has been successfully used in immunohistochemistry (frozen tissue), immunohistochemistry (paraffin tissue), immunoprecipitation applications. The PA1-28653 immunogen is Chicken IgY whole molecule |
| General Information |
| Thermo Scientific Pierce Anti-Chicken secondary antibodies are affinity-purified antibodies with well-characterized specificity for the chicken immunoglobulins and are useful in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies can bind to a single primary antibody. Most commonly, secondary antibodies are generated by immunizing the host animal with a pooled population of immunoglobulins from the target species and can be further purified and modified (i.e. immunoaffinity chromatography, antibody fragmentation, label conjugation, etc.) to generate highly specific reagents. |