Goat anti-Chicken IgY, H&L Secondary Antibody, HRP conjugate
Chicken IgY, H&L Polyclonal Secondary Antibody for DB, ELISA, IHC (F), IHC (P), Western blot
| Details | |
| Host / Isotype: | Goat / IgG |
| Class: | Polyclonal |
| Type: | Secondary Antibody |
| Label: | HRP |
| Species Reactivity: | Chicken (Ck) |
| Immunogen: | Chicken IgG whole molecule |
| Ordering Information | ||||
| Pierce Goat anti-Chicken IgY, H&L Secondary Antibody, HRP conjugate |
| Storage: | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Form: | 1 mg of HRP conjugated antibody (2 mg/ml) in 0.15M NaCl, 0.02M K Phosphate, pH 7.2, with 10 mg/ml BSA and 0.01% sodium azide. |
| Applications | Dilution * |
| ELISA (ELISA) | 1:140,000 |
| Western Blot (WB) | 1:2000-1:20000 |
| Immunohistochemistry (Frozen) (IHC (F)) | 1:1000-1:10000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:1000-1:10000 |
| Dot blot (DB) | Assay Dependent |
| Immunomicroscopy (IM) | Assay-dependent |
|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own experiment using appropriate negative and positive controls.
|
|
| Product Specific Information |
| PA1-28658 detects Chicken IgY H&L from chicken samples. PA1-28658 has been successfully used in ELISA, immunohistochemistry (frozen & paraffin), Western blot, and dot blot applications. The PA1-28658 immunogen is chicken IgY whole molecule. Store at 4ºC short term. For extended storage add 50% glycerol, aliquot and store at -20ºC or below. Avoid freeze/thaw cycles. |
| General Information |
| Thermo Scientific Pierce Anti-Chicken secondary antibodies are affinity-purified antibodies with well-characterized specificity for the chicken immunoglobulins and are useful in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies can bind to a single primary antibody. Most commonly, secondary antibodies are generated by immunizing the host animal with a pooled population of immunoglobulins from the target species and can be further purified and modified (i.e. immunoaffinity chromatography, antibody fragmentation, label conjugation, etc.) to generate highly specific reagents. |
(This product is for In Vitro experimental use only. Not for resale without express authorization.)
HOME >
Chicken IgY, H&L Antibodies > Chicken IgY, H&L Polyclonal Secondary Antibody PA1-28658
