* Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own experiment using appropriate negative and positive controls.
Antigen affinity chromatography
0.1M tris, pH 7,
10% glycerol, 0.1M glycine
-20° C, Avoid Freeze/Thaw Cycles
Product Specific Information
PA5-29325 targets Glucokinase in IF, IHC (P), and WB applications and shows reactivity with Human samples.
The PA5-29325 immunogen is recombinant fragment corresponding to a region within amino acids 1 and 250 of Human Glucokinase.
Hexokinases phosphorylate glucose to produce glucose-6-phosphate, thus committing glucose to the glycolytic pathway. Alternative splicing of this gene results in three tissue-specific forms of glucokinase, one found in pancreatic islet beta cells and two found in liver. The protein localizes to the outer membrane of mitochondria. In contrast to other forms of hexokinase, this enzyme is not inhibited by its product glucose-6-phosphate but remains active while glucose is abundant. Mutations in this gene have been associated with non-insulin dependent diabetes mellitus (NIDDM), maturity onset diabetes of the young, type 2 (MODY2) and persistent hyperinsulinemic hypoglycemia of infancy (PHHI).
Glucokinase Antibody (PA5-29325) in IF Glucokinase_PA5-29325_Antibody_Immunofluorescence.jpg
Immunofluorescence with anti-Glucokinase Polyclonal Antibody (PA5-29325)