Glucokinase Antibody

Glucokinase Polyclonal Antibody for Western blot, IF, ICC, IHC (P)

>> See 4 other antibodies for GCK (FGQTL3, GK, GLK, HHF3, HK4, HKIV, HXKP, LGLK, MODY2, ATP:D-hexose 6-phosphotransferase, HK IV, glucokinase, hexokinase D, pancreatic isozyme, hexokinase type IV, hexokinase-4, hexokinase-D)
Synonyms:
FGQTL3, GK, GLK, HHF3, HK4, HKIV, HXKP, LGLK, MODY2, ATP:D-hexose 6-phosphotransferase, HK IV, glucokinase, hexokinase D, pancreatic isozyme, hexokinase type IV, hexokinase-4, hexokinase-D
Entrez Gene ID:
UniProt ID:
Details
Host / Isotype: Rabbit / IgG
Class: Polyclonal
Type: Antibody
Tested Species Reactivity: Human (Hu)
Immunogen: Recombinant fragment corresponding to a region within amino acids 1 and 250 of Human Glucokinase
Ordering Information
Pierce Glucokinase Antibody
Product #
PA5-29325
Size
100 µL
Price
$324.00
Purchase
Tested Applications Dilution *
Western Blot (WB) 1:500-1:3000
Immunofluorescence (IF) 1:100-1:1000
Immunocytochemistry (ICC) 1:100-1:1000
Immunohistochemistry (Paraffin) (IHC (P)) 1:100-1:1000
* Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own experiment using appropriate negative and positive controls.
Form Information
Form: Liquid
Concentration: 1mg/ml
Purification: Antigen affinity chromatography
Storage Buffer: 0.1M tris, pH 7, with 10% glycerol, 0.1M glycine
Preservative: 0.01% thimerosal
Storage Conditions: -20° C, Avoid Freeze/Thaw Cycles
Product Specific Information
PA5-29325 targets Glucokinase in IF, IHC (P), and WB applications and shows reactivity with Human samples.

The PA5-29325 immunogen is recombinant fragment corresponding to a region within amino acids 1 and 250 of Human Glucokinase.
General Information
Hexokinases phosphorylate glucose to produce glucose-6-phosphate, thus committing glucose to the glycolytic pathway. Alternative splicing of this gene results in three tissue-specific forms of glucokinase, one found in pancreatic islet beta cells and two found in liver. The protein localizes to the outer membrane of mitochondria. In contrast to other forms of hexokinase, this enzyme is not inhibited by its product glucose-6-phosphate but remains active while glucose is abundant. Mutations in this gene have been associated with non-insulin dependent diabetes mellitus (NIDDM), maturity onset diabetes of the young, type 2 (MODY2) and persistent hyperinsulinemic hypoglycemia of infancy (PHHI).
Product Images
  • Glucokinase Antibody (PA5-29325) in WB
    PA5-29325_WB_1.jpg

    Western Blot with anti-Glucokinase Polyclonal Antibody (PA5-29325) (PA5-29325_WB_1.jpg)

    Western Blot with anti-Glucokinase Polyclonal Antibody (PA5-29325)

    Western blot analysis of Glucokinase using A) 30 ?g Huh7 whole cell lysate (B) 30 ?g Hep3B whole cell lysate and C) 30 ?g HepG2 whole cell lysate. Samples were loaded onto a 10% SDS-PAGE gel and probed with a Glucokinase polyclonal antibody (Product # PA5-29325) at a dilution of 1:1000.
  • Glucokinase Antibody (PA5-29325) in WB
    PA5-29325_WB_2.jpg

    Western Blot with anti-Glucokinase Polyclonal Antibody (PA5-29325) (PA5-29325_WB_2.jpg)

    Western Blot with anti-Glucokinase Polyclonal Antibody (PA5-29325)

    Western blot analysis of Glucokinase using 30µg of A) H1299 and B) HeLa lysate. Samples were loaded onto a 10% SDS-PAGE gel and probed with a Glucokinase polyclonal antibody (Product # PA5-29325) at a dilution of 1:1000.
  • Glucokinase Antibody (PA5-29325) in IF
    PA5-29325_IF_1.jpg

    Immunofluorescence with anti-Glucokinase Polyclonal Antibody (PA5-29325) (PA5-29325_IF_1.jpg)

    Immunofluorescence with anti-Glucokinase Polyclonal Antibody (PA5-29325)

    Immunofluorescent analysis of Glucokinase showing staining in the cytoplasm of HeLa cells. HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min and stained using a Glucokinase polyclonal antibody (Product # PA5-29325) diluted at 1:500. Blue: Hoechst 33342 staining.
  • Glucokinase Antibody (PA5-29325) in IF
    PA5-29325_IF_2.jpg

    Immunofluorescence with anti-Glucokinase Polyclonal Antibody (PA5-29325) (PA5-29325_IF_2.jpg)

    Immunofluorescence with anti-Glucokinase Polyclonal Antibody (PA5-29325)

    Immunofluorescent analysis of Glucokinase in paraformaldehyde-fixed A549 cells using a Glucokinase polyclonal antibody (Product # PA5-29325) at a 1:200 dilution.
  • Glucokinase Antibody (PA5-29325) in IHC (P)
    PA5-29325_IHC.jpg

    Immunohistochemistry (Paraffin) with anti-Glucokinase Polyclonal Antibody (PA5-29325) (PA5-29325_IHC.jpg)

    Immunohistochemistry (Paraffin) with anti-Glucokinase Polyclonal Antibody (PA5-29325)

    Immunohistochemical analysis of Glucokinase in paraffin-embedded MCF-7 xenograft using a Glucokinase polyclonal antibody (Product # PA5-29325) at a dilution of 1:500.
(This product is for In Vitro experimental use only. Not for resale without express authorization.)
Part of Thermo Fisher Scientific