Glucokinase Antibody

Glucokinase Polyclonal Antibody for Western blot, IF, ICC, IHC (P)

>> See 4 other antibodies for GCK (FGQTL3, GK, GLK, HHF3, HK4, HKIV, HXKP, LGLK, MODY2, ATP:D-hexose 6-phosphotransferase, HK IV, glucokinase, hexokinase D, pancreatic isozyme, hexokinase type IV, hexokinase-4, hexokinase-D)
Synonyms:
FGQTL3, GK, GLK, HHF3, HK4, HKIV, HXKP, LGLK, MODY2, ATP:D-hexose 6-phosphotransferase, HK IV, glucokinase, hexokinase D, pancreatic isozyme, hexokinase type IV, hexokinase-4, hexokinase-D
Entrez Gene ID:
UniProt ID:
Details
Host / Isotype: Rabbit / IgG
Class: Polyclonal
Type: Antibody
Tested Species Reactivity: Human (Hu)
Immunogen: Recombinant fragment corresponding to a region within amino acids 1 and 250 of Human Glucokinase
Ordering Information
Pierce Glucokinase Antibody
Product #
PA5-29325
Size
100 ul
Price
$316.00
Purchase
Add Glucokinase Antibody to your cart.
Tested Applications Dilution *
Western Blot (WB) 1:500-1:3000
Immunofluorescence (IF) 1:100-1:1000
Immunocytochemistry (ICC) 1:100-1:1000
Immunohistochemistry (Paraffin) (IHC (P)) 1:100-1:1000
* Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own experiment using appropriate negative and positive controls.
Form Information
Form: Liquid
Concentration: 1mg/ml
Purification: Antigen affinity chromatography
Storage Buffer: 0.1M tris, pH 7, with 10% glycerol, 0.1M glycine
Preservative: 0.01% thimerosal
Storage Conditions: -20° C, Avoid Freeze/Thaw Cycles
Product Specific Information
PA5-29325 targets Glucokinase in IF, IHC (P), and WB applications and shows reactivity with Human samples.

The PA5-29325 immunogen is recombinant fragment corresponding to a region within amino acids 1 and 250 of Human Glucokinase.
General Information
Hexokinases phosphorylate glucose to produce glucose-6-phosphate, thus committing glucose to the glycolytic pathway. Alternative splicing of this gene results in three tissue-specific forms of glucokinase, one found in pancreatic islet beta cells and two found in liver. The protein localizes to the outer membrane of mitochondria. In contrast to other forms of hexokinase, this enzyme is not inhibited by its product glucose-6-phosphate but remains active while glucose is abundant. Mutations in this gene have been associated with non-insulin dependent diabetes mellitus (NIDDM), maturity onset diabetes of the young, type 2 (MODY2) and persistent hyperinsulinemic hypoglycemia of infancy (PHHI).
Product Images
  • Glucokinase Antibody (PA5-29325) in WB
    Glucokinase_PA5-29325_Antibody_WesternBlot.jpg

    Western Blot with anti-Glucokinase Polyclonal Antibody (PA5-29325) (Glucokinase_PA5-29325_Antibody_WesternBlot.jpg)

    Western Blot with anti-Glucokinase Polyclonal Antibody (PA5-29325)

    Western Blot analysis using anti-Glucokinase Polyclonal Antibody on the following cells: (30 ug of whole cell lysate) A: H1299 B: Hela. Primary antibody (PA5-29325) diluted at 1:1000
  • Glucokinase Antibody (PA5-29325) in IF
    Glucokinase_PA5-29325_Antibody_Immunofluorescence.jpg

    Immunofluorescence with anti-Glucokinase Polyclonal Antibody (PA5-29325) (Glucokinase_PA5-29325_Antibody_Immunofluorescence.jpg)

    Immunofluorescence with anti-Glucokinase Polyclonal Antibody (PA5-29325)

    Immunofluorescence analysis of paraformaldehyde-fixed A549 cells using Glucokinase (PA5-29325) antibody at 1:200 dilution.
  • Glucokinase Antibody (PA5-29325) in IHC
    Glucokinase_PA5-29325_Antibody_Immunohistochemistry.jpg

    Immunohistochemistry with anti-Glucokinase Polyclonal Antibody (PA5-29325) (Glucokinase_PA5-29325_Antibody_Immunohistochemistry.jpg)

    Immunohistochemistry with anti-Glucokinase Polyclonal Antibody (PA5-29325)

    Immunohistochemical analysis of paraffin-embedded MCF7 xenograft using Glucokinase (PA5-29325) antibody at 1:500 dilution.
(This product is for In Vitro experimental use only. Not for resale without express authorization.)
Part of Thermo Fisher Scientific