Gelsolin Antibody

Gelsolin Polyclonal Antibody for Western blot, IF, ICC, IHC (P)

>> See 7 other antibodies for GSN (ADF, AGEL, actin-depolymerizing factor, brevin)
Synonyms:
ADF, AGEL, actin-depolymerizing factor, brevin
Entrez Gene ID:
Details
Host / Isotype: Rabbit / IgG
Class: Polyclonal
Type: Antibody
Tested Species Reactivity: Human (Hu), Mouse (Ms), Rat (Rt)
Immunogen: Recombinant fragment corresponding to a region within amino acids 561 and 782 of Human Gelsolin
Ordering Information
Pierce Gelsolin Antibody
Product #
PA5-27350
Size
100 µL
Price
$324.00
Purchase
Tested Applications Dilution *
Western Blot (WB) 1:500-1:3000
Immunofluorescence (IF) 1:100-1:1000
Immunocytochemistry (ICC) 1:100-1:1000
Immunohistochemistry (Paraffin) (IHC (P)) 1:100-1:1000
* Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own experiment using appropriate negative and positive controls.
Form Information
Form: Liquid
Concentration: 0.27mg/ml
Purification: Antigen affinity chromatography
Storage Buffer: 0.1M tris, pH 7, with 10% glycerol, 0.1M glycine
Preservative: 0.01% thimerosal
Storage Conditions: -20° C, Avoid Freeze/Thaw Cycles
Product Specific Information
PA5-27350 targets Gelsolin in IF, IHC (P), and WB applications and shows reactivity with Human, rat, and mouse samples.

The PA5-27350 immunogen is recombinant fragment corresponding to a region within amino acids 561 and 782 of Human Gelsolin.
General Information
The protein encoded by this gene binds to the "plus" ends of actin monomers and filaments to prevent monomer exchange. The encoded calcium-regulated protein functions in both assembly and disassembly of actin filaments. Defects in this gene are a cause of familial amyloidosis Finnish type (FAF). Multiple transcript variants encoding several different isoforms have been found for this gene.
Product Images
  • Gelsolin Antibody (PA5-27350) in WB
    PA5-27350_WB_2.jpg

    Western Blot with anti-Gelsolin Polyclonal Antibody (PA5-27350) (PA5-27350_WB_2.jpg)

    Western Blot with anti-Gelsolin Polyclonal Antibody (PA5-27350)

    Western blot analysis of Gelsolin using 30µg of A) H1299 and B) HeLa lysate. Samples were loaded onto a 7.5% SDS-PAGE gel and probed with a Gelsolin polyclonal antibody (Product # PA5-27350) at a dilution of 1:1000.
  • Gelsolin Antibody (PA5-27350) in WB
    PA5-27350_WB_1.jpg

    Western Blot with anti-Gelsolin Polyclonal Antibody (PA5-27350) (PA5-27350_WB_1.jpg)

    Western Blot with anti-Gelsolin Polyclonal Antibody (PA5-27350)

    Western blot analysis of Gelsolin using 30µg of NIH-3T3 lysate. Samples were loaded onto a 7.5% SDS-PAGE gel and probed with a Gelsolin polyclonal antibody (Product # PA5-27350) at a dilution of 1:1000.
  • Gelsolin Antibody (PA5-27350) in IF
    PA5-27350_IF.jpg

    Immunofluorescence with anti-Gelsolin Polyclonal Antibody (PA5-27350) (PA5-27350_IF.jpg)

    Immunofluorescence with anti-Gelsolin Polyclonal Antibody (PA5-27350)

    Immunofluorescent analysis of Gelsolin in methanol-fixed HeLa cells using a Gelsolin polyclonal antibody (Product # PA5-27350) (Green) at a 1:500 dilution. Alpha-tubulin filaments were labeled with Product # PA5-29281 (Red) at a 1:2000.
  • Gelsolin Antibody (PA5-27350) in IHC (P)
    PA5-27350_IHC.jpg

    Immunohistochemistry (Paraffin) with anti-Gelsolin Polyclonal Antibody (PA5-27350) (PA5-27350_IHC.jpg)

    Immunohistochemistry (Paraffin) with anti-Gelsolin Polyclonal Antibody (PA5-27350)

    Immunohistochemical analysis of Gelsolin in paraffin-embedded H1299 xenograft using a Gelsolin polyclonal antibody (Product # PA5-27350) at a dilution of 1:500.
(This product is for In Vitro experimental use only. Not for resale without express authorization.)
Part of Thermo Fisher Scientific