GST Tag Antibody (8-326)

GST Tag Monoclonal Antibody for Western blot, IP

Synonyms:
Glutathione-S-Transferase, GST protein, GST Tag
Details
Host / Isotype: Mouse / IgG
Class: Monoclonal
Type: Antibody
Clone: 8-326
Tested Species Reactivity: Tag (Tag)
Published Species Reactivity: Not Applicable (N/A)
Immunogen: Recombinant purified full-length GST
Ordering Information
Pierce GST Tag Antibody (8-326)
Product #
MA4-004
Size
100 µg
Price
$149.00
Purchase
Add GST Tag Antibody (8-326) to your cart.
Tested Applications Dilution *
Western Blot (WB) 1:1000
Immunoprecipitation (IP) 2 µg
Published Applications Dilution
Western Blot (WB) See publications below
Immunoprecipitation (IP) See publications below
ELISA (ELISA) See publications below
* Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own experiment using appropriate negative and positive controls.
Form Information
Form: Liquid
Concentration: 1mg/ml
Purification: Protein G
Storage Buffer: PBS with 1mg/ml BSA
Preservative: 0.05% sodium azide
Storage Conditions: -20°C
Product Specific Information
General Information
Epitope tags provide a method to localize gene products in a variety of cell types, study the topology of proteins and protein complexes, identify associated proteins, and characterize newly identified, low abundance or poorly immunogenic proteins when protein specific antibodies are not available. The GST (Glutathione-S-Transferase) is a 26kDa protein encoded by the parasite Schistosoma japonicum, easily purified by affinity chromatography on immobilized glutathione. GST is utilized as a fusion protein with foreign proteins in a range of prokaryotic expression vectors, including the pGEX family of vectors. Antibodies against GST will detect the expressed GST protein or a GST-fusion protein, and can be utilized for immuno-purification of GST-fusion proteins.
Product Images
  • GST Tag Antibody (MA4-004) in WB
    MA4-004-Anti-Glutathione-S-transferase-Monoclonal-Antibody-Western-Blot.jpg

    Western Blot with anti-GST Tag Monoclonal Antibody [8-326] (MA4-004) (MA4-004-Anti-Glutathione-S-transferase-Monoclonal-Antibody-Western-Blot.jpg)

    Western Blot with anti-GST Tag Monoclonal Antibody [8-326] (MA4-004)

    Western blot analysis of GST-Abl SH2 domain (Lane 1, 35kDa) (Product # 87702) and GST-Grb2 (Lane 2, 38kDa) SH2 domain (Product #87700) was performed by loading 2ug of each purified fusion protein onto a 4-20% Tris-HCl polyacrylamide gel. Proteins were transferred to a PVDF membrane and blocked with 5% BSA/0.1%TBST for at least 1 hour. Membranes were then probed with a mouse monoclonal antibody recognizing GST clone 8-826 (Product # MA4-004) at a dilution of 1:1000 overnight at 4°C on a rocking platform. Membranes were then washed in TBS/0.1%Tween-20 and probed with a goat anti-mouse-HRP secondary antibody (Product #31430) at a dilution of 1:25000 for at least one hour. Membranes were washed and chemiluminescent detection was performed using Pierce SuperSignal West Dura (Product #34075).
  • GST Tag Antibody (MA4-004) in IP
    MA4-004-Anti-Glutathione-S-transferase-Monoclonal-Antibody-Immunoprecipitation.jpg

    Immunoprecipitation with anti-GST Tag Monoclonal Antibody [8-326] (MA4-004) (MA4-004-Anti-Glutathione-S-transferase-Monoclonal-Antibody-Immunoprecipitation.jpg)

    Immunoprecipitation with anti-GST Tag Monoclonal Antibody [8-326] (MA4-004)

    Immuno-precipitation of GST-Shp2 SH2 domain (Product #87709) (Lane 2, 39kDa) was performed by mixing 2µg of purified fusion protein with1µg of mouse monoclonal antibody recognizing GST (Product # MA4-004) overnight on a rocking platform at 4°C. The immune-complex was then captured on 50µl Protein A/G Plus Agarose (Product #20423). Captured immune-complexes were then washed extensively and proteins eluted with 5X Reducing Sample Loading Dye (Product # 39000). Samples were then resolved on a 4-20% Tris-HCl polyacrylamide gel. IP efficiency was compared to a non-IP loading control consisting of 1ug GST-Shp2 SH2 domain (Lane1). Proteins were transferred to PVDF membrane and blocked with 5% BSA/TBS-0.1%Tween for at least 1 hour. Membranes were then probed with a mouse monoclonal antibody recognizing GST (Product # MA4-004) at a dilution of 1:1000 overnight rotating at 4°C. Membranes were then washed in TBS/0.1% Tween-20 and probed with Clean-blot IP detection reagent (Product #21230) at a dilution of 1:2000 for at least one hour. Membranes were washed and chemiluminescent detection was performed using Pierce Super Signal West Dura (Product #34075).
Publications:
Western Blot
  Species / Dilution Summary
  N/A / 0
MA4-004 was used in western blot to study the mechanism by which E2 rapidly activates MAPK in breast cancer cells

Mol Endocrinol. 2002 Jan;16(1):116-27.
"Linkage of rapid estrogen action to MAPK activation by ERalpha-Shc association and Shc pathway activation."
Author(s): Song RX, McPherson RA, Adam L, Bao Y, Shupnik M, Kumar R, Santen RJ
Number of Citations: 65
(See PubMed article )
  N/A / 0
MA4-004 was used in western blot to study Mcm10 homocomplex assembly

J Biol Chem. 2003 Sep 19;278(38):36051-8.
"A novel zinc finger is required for Mcm10 homocomplex assembly."
Author(s): Cook CR, Kung G, Peterson FC, Volkman BF, Lei M
Number of Citations: 13
(See PubMed article )
  N/A / 0
MA4-004 was used in western blot to study the role of synaptaphillin in dynamin-dependent endocytosis

J Biol Chem. 2003 Oct 17;278(42):41221-6.
"Syntaphilin binds to dynamin-1 and inhibits dynamin-dependent endocytosis."
Author(s): Das S, Gerwin C, Sheng ZH
Number of Citations: 2
(See PubMed article )
  N/A / 0.1 ug/ml
MA4-004 was used in western blot to investigate homo-oligomerization of human corneodesmosin

J Invest Dermatol. 2004 Mar;122(3):747-54.
"Homo-oligomerization of human corneodesmosin is mediated by its N-terminal glycine loop domain."
Author(s): Caubet C, Jonca N, Lopez F, Estève JP, Simon M, Serre G
Number of Citations: 2
(See PubMed article )
  N/A / 1:1000
MA4-004 was used in ELISA and western blot to study the interaction of smooth muscle apha-actinin with smitin

Int J Biochem Cell Biol. 2005 Jul;37(7):1470-82.
"Smooth muscle alpha-actinin interaction with smitin."
Author(s): Chi RJ, Olenych SG, Kim K, Keller TC 3rd
Number of Citations: 2
(See PubMed article )
  N/A / 1:1000
MA4-004 was used in western blot to investigate the role of RING-H2 proteins in ubiquitination

J Virol. 2005 Jul;79(14):8764-72.
"RING-H2 protein WSSV249 from white spot syndrome virus sequesters a shrimp ubiquitin-conjugating enzyme, PvUbc, for viral pathogenesis."
Author(s): Wang Z, Chua HK, Gusti AA, He F, Fenner B, Manopo I, Wang H, Kwang J
Number of Citations: 9
(See PubMed article )
  N/A / 1:1000
MA4-004 was used in western blot to determine whether GST can be phosphorylated by PKC-alpha

Biotechnol Lett. 2005 Dec;27(23-24):1869-73.
"Phosphorylation of glutathione-S-transferase by protein kinase C-alpha implications for affinity-tag purification."
Author(s): Rodriguez P, Mitton B, Kranias EG
Number of Citations: 3
(See PubMed article )
  N/A / 0
MA4-004 was used in western blot to study the expression and antigenicity of ribosomal protein S4 from Trypanosoma cruzi

Mem Inst Oswaldo Cruz. 2007 Jun;102(4):473-9.
"Trypanosoma cruzi ribosomal protein S4: characterization of its coding locus, analysis of transcripts, and antigenicity of the protein."
Author(s): Pérez-Escobar M, Cevallos AM, Espinoza B, Espinosa N, Martínez I, Hernández R
Number of Citations: 0
(See PubMed article )
  N/A / 0
MA4-004 was used in western blot to develop an antigen-capture ELISA for detection of H7 subtype avian influenza

J Virol Methods. 2008 Feb;147(2):219-25.
"Development of an antigen-capture ELISA for detection of H7 subtype avian influenza from experimentally infected chickens."
Author(s): Velumani S, Du Q, Fenner BJ, Prabakaran M, Wee LC, Nuo LY, Kwang J
Number of Citations: 7
(See PubMed article )
  N/A / 1:1000
MA4-004 was used in western blot to study structural interactions between proteins within smooth muscle

J Biol Chem. 2008 Jul 25;283(30):20959-67.
"Smooth muscle titin Zq domain interaction with the smooth muscle alpha-actinin central rod."
Author(s): Chi RJ, Simon AR, Bienkiewicz EA, Felix A, Keller TC 3rd
Number of Citations: 2
(See PubMed article )
  N/A / Not Cited
MA4-004 was used in western blot to investigate the association between WSP-like protein WSP-0284 and Brugia malayi proteins

Int J Parasitol. 2011 Aug 15;41(10):1053-61.
"Interaction of a Wolbachia WSP-like protein with a nuclear-encoded protein of Brugia malayi."
Author(s): Melnikow E, Xu S, Liu J, Li L, Oksov Y, Ghedin E, Unnasch TR, Lustigman S
Number of Citations: 0
(See PubMed article )
  N/A / 0
MA4-004 was used in western blot to study the role of AUCSIA-1 in Arabidopsis

PLoS One. 2012;7(7):e41327.
"Arabidopsis thaliana AUCSIA-1 regulates auxin biology and physically interacts with a kinesin-related protein."
Author(s): Molesini B, Pandolfini T, Pii Y, Korte A, Spena A
Number of Citations: 2
(See PubMed article )
  N/A / 0
MA4-004 was used in western blot to study the effects of chronic binge-like ethanol on umbilical eNOS under basal and ATP-stimulated conditions

Reprod Toxicol. 2014 Jan;43():94-101.
"Interactive effects of in vitro binge-like alcohol and ATP on umbilical endothelial nitric oxide synthase post-translational modifications and redox modulation."
Author(s): Subramanian K, Naik VD, Sathishkumar K, Sawant OB, Washburn SE, Wu G, Yallampalli C, Saade GR, Hankins GD, Ramadoss J
Number of Citations: 0
(See PubMed article )
Immunoprecipitation
  Species / Dilution Summary
  N/A / 0
MA4-004 was used in immunoprecipitation to study interactions between host factors and hepadnavirus core protein

PLoS One. 2011;6(12):e29566.
"Phosphorylation state-dependent interactions of hepadnavirus core protein with host factors."
Author(s): Ludgate L, Adams C, Hu J
Number of Citations: 1
(See PubMed article )
ELISA
  Species / Dilution Summary
  N/A / 1:500
MA4-004 was used in ELISA and western blot to study the interaction of smooth muscle apha-actinin with smitin

Int J Biochem Cell Biol. 2005 Jul;37(7):1470-82.
"Smooth muscle alpha-actinin interaction with smitin."
Author(s): Chi RJ, Olenych SG, Kim K, Keller TC 3rd
Number of Citations: 2
(See PubMed article )
  N/A / 0
MA4-004 was used in ELISA to study the functional significance of the interaction of ESCRT III with LIP5 and VPS4

Mol Biol Cell. 2008 Jun;19(6):2661-72.
"Novel interactions of ESCRT-III with LIP5 and VPS4 and their implications for ESCRT-III disassembly."
Author(s): Shim S, Merrill SA, Hanson PI
Number of Citations: 29
(See PubMed article )
(This product is for In Vitro experimental use only. Not for resale without express authorization.)
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