GLUT10 Antibody

GLUT10 Polyclonal Antibody for Western blot, IF, ICC

>> See 2 other antibodies for SLC2A10 (GLUT10, GLUT11, MGC118830, MGC118833, SLC2A11-a, SLC2A11-c)
Synonyms:
GLUT10, GLUT11, MGC118830, MGC118833, SLC2A11-a, SLC2A11-c
Entrez Gene ID:
UniProt ID:
Details
Host / Isotype: Rabbit
Class: Polyclonal
Type: Antibody
Tested Species Reactivity: Human (Hu), Mouse (Ms)
Immunogen: Residues 367-385 of the human GLUT10 protein.
Ordering Information
Pierce GLUT10 Antibody
Product #
PA1-46137
Size
100 µg
Price
$285.00
Purchase
Add GLUT10 Antibody to your cart.
Tested Applications Dilution *
Western Blot (WB) 1:500 - 1:1,000
Immunofluorescence (IF) Assay Dependent
Immunocytochemistry (ICC) 1:10-1:2000
* Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own experiment using appropriate negative and positive controls.
Form Information
Form: Liquid
Concentration: 1mg/ml
Purification: Antigen affinity chromatography
Storage Buffer: PBS
Preservative: 0.02% sodium azide
Storage Conditions: Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Product Specific Information
General Information
Non-insulin-dependent diabetes mellitus (NIDDM) is a multifactoral disease with both environmental and genetics causes. Genome-wide screening procedures have identified several susceptibility loci for NIDDM within the human genome. A putative sugar transp
Product Images
  • GLUT10 Antibody (PA1-46137) in WB
    SLC2A11_Antibody_PA1-46137_WesternBlot(WB)_1.jpg

    Western Blot with anti-GLUT10 Polyclonal Antibody (PA1-46137) (SLC2A11_Antibody_PA1-46137_WesternBlot(WB)_1.jpg)

    Western Blot with anti-GLUT10 Polyclonal Antibody (PA1-46137)

  • GLUT10 Antibody (PA1-46137) in WB
    SLC2A11_Antibody_PA1-46137_WesternBlot(WB)_2.jpg

    Western Blot with anti-GLUT10 Polyclonal Antibody (PA1-46137) (SLC2A11_Antibody_PA1-46137_WesternBlot(WB)_2.jpg)

    Western Blot with anti-GLUT10 Polyclonal Antibody (PA1-46137)

  • GLUT10 Antibody (PA1-46137) in WB
    PA1-46137_WesternBlot.jpg

    Western Blot with anti-GLUT10 Polyclonal Antibody (PA1-46137) (PA1-46137_WesternBlot.jpg)

    Western Blot with anti-GLUT10 Polyclonal Antibody (PA1-46137)

    Cochlea from embryonic mice and mice up to 1 year old were removed and the pure membrane fraction loaded on 4-20% SDS gel, Following electrophoresis, transfer and blocking, the blot was incubated overnight at 4 degrees C with the GLUT10 primary antibody at a 1:500 dilution followed by incubation with secondary antibody at a 1:3,000 dilution for 1 h at room temperature. Protein bands were visualized on x-ray film with supersignal west femto chemiluminescent substrate. Lane 1. 10ug of protein from liver 2. 10ug of protein from cochlea (E18) 3. 10ug of protein from cochlea (P7) 4. 10ug of protein from cochlea (P10) 5. 10ug of protein from cochlea (P14) 6. 10ug of protein from cochlea (P17) 7. 10ug of protein from cochlea (P60) 8. 10ug of protein from cochlea (1 Year)
  • GLUT10 Antibody (PA1-46137) in WB
    PA1-46137_WesternBlot2.jpg

    Western Blot with anti-GLUT10 Polyclonal Antibody (PA1-46137) (PA1-46137_WesternBlot2.jpg)

    Western Blot with anti-GLUT10 Polyclonal Antibody (PA1-46137)

    NCI-H1299 non-small cell lung tumor cells were transfected with the eukaryotic expression vectors pcDNA3.1/V5 (lanes 1,2), pCMV-GLUT10 (lanes 3,4), and pCMV-GLUT1 (lanes 5,6). GLUT10 was detetced using 1.
  • GLUT10 Antibody (PA1-46137) in IF
    SLC2A11_Antibody_PA1-46137_Immunofluorescence(IF).jpg

    Immunofluorescence with anti-GLUT10 Polyclonal Antibody (PA1-46137) (SLC2A11_Antibody_PA1-46137_Immunofluorescence(IF).jpg)

    Immunofluorescence with anti-GLUT10 Polyclonal Antibody (PA1-46137)

  • GLUT10 Antibody (PA1-46137) in IHC
    PA1-46137_Immunohistochemistry.jpg

    Immunohistochemistry with anti-GLUT10 Polyclonal Antibody (PA1-46137) (PA1-46137_Immunohistochemistry.jpg)

    Immunohistochemistry with anti-GLUT10 Polyclonal Antibody (PA1-46137)

    Dissected inner ear tissues from mice were immersed in 20% sucrose PBS solution (pH 7.4) overnight followed by being embedded in OCT overnight. The tissues were snap frozen in liquid nitrogen and 7um sections were cut by cryostat. Cochlear sections from the organ of Corti were dissected and rinsed by 0.1% Triton in PBS (pH 7.4, PBST) for 30 minutes. Sections were blocked with 5% goat serum in PBST at room temperature for 1 hour. The GLUT10 antibody was used at a 1:200 dilution to label the cochlear sections at 4 degrees C overnight. After washing, the sections were incubated with secondary antibodies (anti-rabbit Cy3, 1:400) at room temperature for 1 hour. Cy3-conjugated secondary antibodies were used to visualize the binding of the primary antibodies. The slides were mounted with fluoromont G and examined under a confocal microscope. Data from cochlear sections show strong immunoreactivity (stained red) is observed in the inner hair cells, outer hair cells and basilar membrane. In addition, spiral ganglion neurons display moderate staining.
(This product is for In Vitro experimental use only. Not for resale without express authorization.)
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