GCK Antibody (4G6)

GCK Monoclonal Antibody for Western blot

>> See 4 other antibodies for GCK (FGQTL3, GK, GLK, HHF3, HK4, HKIV, HXKP, LGLK, MODY2, ATP:D-hexose 6-phosphotransferase; HK IV; glucokinase; hexokinase D, pancreatic isozyme; hexokinase type IV; hexokinase-4; hexokinase-D)
Synonyms:
FGQTL3, GK, GLK, HHF3, HK4, HKIV, HXKP, LGLK, MODY2, ATP:D-hexose 6-phosphotransferase; HK IV; glucokinase; hexokinase D, pancreatic isozyme; hexokinase type IV; hexokinase-4; hexokinase-D
Entrez Gene ID:
UniProt ID:
Details
Host / Isotype: Mouse / IgG1
Class: Monoclonal
Type: Antibody
Clone: 4G6
Tested Species Reactivity: Human (Hu)
Immunogen: Purified recombinant fragment of human GCK expressed in E. Coli.
Ordering Information
Pierce GCK Antibody (4G6)
Product #
MA5-15878
Size
100 ul
Price
$316.00
Purchase
Add GCK Antibody (4G6) to your cart.
Tested Applications Dilution *
Western Blot (WB) 1/500 - 1/2000
* Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own experiment using appropriate negative and positive controls.
Form Information
Form: Liquid
Storage Buffer: ascites
Preservative: 0.03% sodium azide
Storage Conditions: Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Product Specific Information
MA5-15878 targets GCK in WB applications and shows reactivity with Human samples.

The MA5-15878 immunogen is purified recombinant fragment of human GCK expressed in E. Coli. .

MA5-15878 detects GCK which has a predicted molecular weight of approximately 52kDa.
General Information
Hexokinases phosphorylate glucose to produce glucose-6-phosphate, the first step in most glucose metabolism pathways. Alternative splicing of this gene results in three tissue-specific forms of glucokinase, one found in pancreatic islet beta cells and two found in liver. The protein localizes to the outer membrane of mitochondria. In contrast to other forms of hexokinase, this enzyme is not inhibited by its product glucose-6-phosphate but remains active while glucose is abundant. Mutations in this gene have been associated with non-insulin dependent diabetes mellitus (NIDDM), maturity onset diabetes of the young, type 2 (MODY2) and persistent hyperinsulinemic hypoglycemia of infancy (PHHI).
Product Images
  • GCK Antibody (MA5-15878) in WB
    GCK_Antibody_MA5-15878_Westernblot1-1252013.jpg

    Western Blot with anti-GCK Monoclonal Antibody [4G6] (MA5-15878) (GCK_Antibody_MA5-15878_Westernblot1-1252013.jpg)

    Western Blot with anti-GCK Monoclonal Antibody [4G6] (MA5-15878)

    Western blot analysis of GCK using a GCK monoclonal antibody (Product # MA5-15878) against a human GCK (AA: 1-198) recombinant protein.
  • GCK Antibody (MA5-15878) in WB
    GCK_Antibody_MA5-15878_Westernblot2-1252013.jpg

    Western Blot with anti-GCK Monoclonal Antibody [4G6] (MA5-15878) (GCK_Antibody_MA5-15878_Westernblot2-1252013.jpg)

    Western Blot with anti-GCK Monoclonal Antibody [4G6] (MA5-15878)

    Western blot analysis of GCK using GCK monoclonal antibody (Product # MA5-15878) in HEK293 (1) and GCK-human IgG Fc transfected HEK293 (2) cell lysate.
(This product is for In Vitro experimental use only. Not for resale without express authorization.)
Part of Thermo Fisher Scientific