* Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own experiment using appropriate negative and positive controls.
Antigen affinity chromatography
1mg/ml BSA, 30% glycerol
0.05% sodium azide
Product Specific Information
PA1-104 has been successfully used in Western blot, IHC (P) and immunofluorescence applications with human and mouse samples.
This gene encodes a member of the GATA family of zinc-finger transcription factors that are named for the consensus nucleotide sequence they bind in the promoter regions of target genes. GATA6 is thought to be important for regulating terminal differentiation and/or proliferation and plays a critical role in endoderm development.
GATA6 Antibody (PA1-104) in WB PA11-4_WB.jpg
Western Blot with anti-GATA6 Polyclonal Antibody (PA1-104)
Western blot analysis of GATA6 was performed by loading 25ug of various whole cell lysate onto a 4-20% Tris-HCl polyacrylamide gel. Proteins were transferred to a PVDF membrane and blocked with 5% BSA/TBST for at least 1 hour. Membranes were then probed with a rabbit polyclonal antibody recognizing GATA6 (Product # PA1-104) at a dilution of 1:1000 overnight at 4°C on a rocking platform. Membranes were then washed in TBS-0.1%Tween 20 and probed with a goat anti-rabbit-HRP secondary antibody (Product #31460) at a dilution of 1:15,000 for at least one hour. Membranes were washed and chemiluminescent detection was performed using SuperSignal West Pico (Product # 34080).
GATA6 Antibody (PA1-104) in IF PA1104_IF.jpg
Immunofluorescence with anti-GATA6 Polyclonal Antibody (PA1-104)
Immunofluorescent analysis of GATA6 (green) in HeLa cells. Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature. Cells were then blocked with 5% normal goat serum (Product #31873) for 15 minutes at room temperature. Cells were then probed with a rabbit polyclonal antibody recognizing GATA6 (Product # PA1-104) at a dilution of 1:200 for at least 1 hour at 37°C. Cells were then washed with PBS and incubated with DyLight 488 goat-anti-rabbit secondary antibody at a dilution of 1:200 for 30 minutes at room temperature. Nuclei (blue) were stained with Hoechst 33342 dye (Product# 62249). Images were taken on a Thermo Scientific ArrayScan at 10X magnification.
GATA6 Antibody (PA1-104) in IHC PA1-104_IHC_Human-Stomach tissue.jpg
Immunohistochemistry with anti-GATA6 Polyclonal Antibody (PA1-104)
Immunohistochemistry was performed on human stomach tissue. To expose target protein, antigen was retreived using 10mM sodium citrate followed by microwave treatment for 8-15 minutes. Endogenous peroxidases were blocked in 3% H202-methanol for 15 minutes and tissues were blocked in 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with a GATA6 Rabbit polyclonal antibody (Product # PA1-104) at a dilution of 1:100 overnight in a humidified chamber. Tissues were washed in PBST and detection was performed using a secondary antibody conjugated to HRP. DAB staining buffer was applied and tissues were counterstained with hematoxylin and prepped for mounting. Images were taken at 40X magnification.
(This product is for In Vitro experimental use only. Not for resale without express authorization.)