GAPDH Antibody (1D4)

(This product has been discontinued.)
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>> See 16 other antibodies for GAPDH

GAPDH Monoclonal Antibody for Western blot, IF

>> See 16 other antibodies for GAPDH (Glyceraldehyde-3 Phosphate Dehydrogenase)
Synonyms:
Glyceraldehyde-3 Phosphate Dehydrogenase
Details
Host / Isotype: Mouse / IgM, kappa
Class: Monoclonal
Type: Antibody
Clone: 1D4
Tested Species Reactivity: Human (Hu), Mouse (Ms), Rat (Rt), Avian (Av), Bovine (Bv), Porcine (Po)
Published Species Reactivity: Amphibian (Am), Bovine (Bv), Human (Hu)
Immunogen: Extensively purified pig GAPDH.
Ordering Information
Pierce GAPDH Antibody (1D4)
Product #
MA1-10036
Size
500 µl
Price
$339.00
Purchase
(DISCONTINUED)
Tested Applications Dilution *
Western Blot (WB) 1:1,000 or less
Immunofluorescence (IF) 1:100
Published Applications Dilution
Western Blot (WB) See publications below
Immunocytochemistry (ICC) See publications below
Immunohistochemistry (IHC) See publications below
Immunoprecipitation (IP) See publications below
ELISA (ELISA) See publications below
* Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own experiment using appropriate negative and positive controls.
Form Information
Form information is not available for this product.
Product Specific Information
MA1-10036 detects Glyceraldehyde-3 Phosphate Dehydrogenase from human, avian, mouse, rat, bovine, and porcine samples.

MA1-10036 has been successfully used in immunofluorescence and Western blot applications.

The MA1-10036 immunogen is extensively purified pig GAPDH.

General Information
Glyceraldehyde 3-Phosphate Dehydrogenase (GAPDH) is a metabolic enzyme responsible for catalyzing one step in the glycolytic pathway, the reversible oxidative phosphorylation of glyceraldehyde 3-phosphate. Because GAPDH as a protein expressed in large amounts and which is required at all times for an important "house keeping" functions, levels of GAPDH mRNA are often measured and used as standards for studies of mRNA expression. Increasingly, scientists are making use of specific antibodies to GAPDH in comparable studies of levels of protein expression. This antibody can be used as a loading control for western blotting experiments, allowing comparison between the level of this protein and others in a cell or tissue. Depart from a role in glycolysis, GAPDH may have other roles such as in the activation of transcription. GAPDH is reported to bind to a variety of other proteins, including amyloid precursor protein, mutations in which cause some forms of Alzheimer's disease, and the polyglutamine tracts of Huntingtin, the protein product aberrrant forms of which are causative of Huntingtin's disease. Associations with actin and tubulin have also been reported. The protein may also have a role in the regulationof apoptosis, and interetingly migrates from the cytoplasm into the nucleas when cells become apoptotic.
Product Images
  • GAPDH Antibody (MA1-10036) in IHC
    GAPDH_Antibody_MA1-10036_Immunohistochemical(IHC).jpg

    Immunohistochemistry with anti-GAPDH Monoclonal Antibody [1D4] (MA1-10036) (GAPDH_Antibody_MA1-10036_Immunohistochemical(IHC).jpg)

    Immunohistochemistry with anti-GAPDH Monoclonal Antibody [1D4] (MA1-10036)

Publications:
Western Blot
  Species / Dilution Summary
  Am / Not Cited
Bv / Not Cited
MA1-10036 was used in western blot, ELISA and immunoprecipitation to produce and characterize novel anti-rhodopsin monoclonal antibodies

Biochemistry. 1983 Feb 1;22(3):653-60.
"Monoclonal antibodies to rhodopsin: characterization, cross-reactivity, and application as structural probes."
Author(s): Molday RS, MacKenzie D
Number of Citations: 55
(See PubMed article )
  Bv / Not Cited
MA1-10036 was used in immunohistochemistry and western blot to perform mutational analysis on bovine opsin

Biochem J. 1996 Dec 15;320 ( Pt 3)():807-15.
"Point mutations in bovine opsin can be classified in four groups with respect to their effect on the biosynthetic pathway of opsin."
Author(s): DeCaluwé GL, DeGrip WJ
Number of Citations: 1
(See PubMed article )
  Bv / Not Cited
MA1-10036 was used in immunoprecipitation and western blot to investigate the synthesis and transportation of rhodopsin-GFP in vivo

J Biol Chem. 2001 Jul 27;276(30):28242-51.
"A functional rhodopsin-green fluorescent protein fusion protein localizes correctly in transgenic Xenopus laevis retinal rods and is expressed in a time-dependent pattern."
Author(s): Moritz OL, Tam BM, Papermaster DS, Nakayama T
Number of Citations: 1
(See PubMed article )
Immunocytochemistry
  Species / Dilution Summary
  Hu / 1:100
MA1-10036 was used in immunocytochemistry to study the mechanism for rod and cone photoreceptor degeneration from rhodopsin mutations

Proc Natl Acad Sci U S A. 1998 Jun 9;95(12):7103-8.
"Disease sequence from mutant rhodopsin allele to rod and cone photoreceptor degeneration in man."
Author(s): Cideciyan AV, Hood DC, Huang Y, Banin E, Li ZY, Stone EM, Milam AH, Jacobson SG
Number of Citations: 23
(See PubMed article )
Immunohistochemistry
  Species / Dilution Summary
  Bv / Not Cited
MA1-10036 was used in immunohistochemistry and western blot to perform mutational analysis on bovine opsin

Biochem J. 1996 Dec 15;320 ( Pt 3)():807-15.
"Point mutations in bovine opsin can be classified in four groups with respect to their effect on the biosynthetic pathway of opsin."
Author(s): DeCaluwé GL, DeGrip WJ
Number of Citations: 1
(See PubMed article )
Immunoprecipitation
  Species / Dilution Summary
  Bv / Not Cited
MA1-10036 was used in western blot, ELISA and immunoprecipitation to produce and characterize novel anti-rhodopsin monoclonal antibodies

Biochemistry. 1983 Feb 1;22(3):653-60.
"Monoclonal antibodies to rhodopsin: characterization, cross-reactivity, and application as structural probes."
Author(s): Molday RS, MacKenzie D
Number of Citations: 55
(See PubMed article )
  Bv / Not Cited
MA1-10036 was used in immunoprecipitation and western blot to investigate the synthesis and transportation of rhodopsin-GFP in vivo

J Biol Chem. 2001 Jul 27;276(30):28242-51.
"A functional rhodopsin-green fluorescent protein fusion protein localizes correctly in transgenic Xenopus laevis retinal rods and is expressed in a time-dependent pattern."
Author(s): Moritz OL, Tam BM, Papermaster DS, Nakayama T
Number of Citations: 1
(See PubMed article )
ELISA
  Species / Dilution Summary
  Bv / Not Cited
MA1-10036 was used in ELISA to investigate the specificity of monoclonal anti-rhodopsin antibodies

J Biol Chem. 1988 Aug 25;263(24):11768-75.
"Antigen-antibody interaction. Synthetic peptides define linear antigenic determinants recognized by monoclonal antibodies directed to the cytoplasmic carboxyl terminus of rhodopsin."
Author(s): Hodges RS, Heaton RJ, Parker JM, Molday L, Molday RS
Number of Citations: 18
(See PubMed article )
  Bv / Not Cited
MA1-10036 was used in western blot, ELISA and immunoprecipitation to produce and characterize novel anti-rhodopsin monoclonal antibodies

Biochemistry. 1983 Feb 1;22(3):653-60.
"Monoclonal antibodies to rhodopsin: characterization, cross-reactivity, and application as structural probes."
Author(s): Molday RS, MacKenzie D
Number of Citations: 55
(See PubMed article )
(This product is for In Vitro experimental use only. Not for resale without express authorization.)
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