GAPDH Antibody (PA1-988) in WB
PA1-988_WB_20131209144802.jpgWestern blot analysis of GAPDH was performed by loading 25 ug of Hela (lane 1) and C6 (lane 2) cell lysates onto an SDS polyacrylamide gel. Proteins were transferred to a PVDF membrane and blocked at 4ºC overnight. The membrane was probed with a GAPDH polyclonal antibody (Product # PA1-988) at a dilution of 1:1000 overnight at 4°C, washed in TBST, and probed with an HRP-conjugated secondary antibody for 1 hr at room temperature in the dark. Chemiluminescent detection was performed using Pierce ECL Plus Western Blotting Substrate (Product # 32132). Results show a band at ~36 kDa.