GAPDH Antibody

GAPDH Polyclonal Antibody for Western blot

>> See 15 other antibodies for GAPDH (Glyceraldehyde-3-Phosphate Dehydrogenase, GAPD, G3PD, G3PDH)
Synonyms:
Glyceraldehyde-3-Phosphate Dehydrogenase, GAPD, G3PD, G3PDH
Entrez Gene ID:
Details
Host / Isotype: Rabbit
Class: Polyclonal
Type: Antibody
Tested Species Reactivity: Human (Hu), Mouse (Ms), Rat (Rt)
Published Species Reactivity: Mouse (Ms)
Immunogen: Synthetic peptide: V(4) K V G V N G F G R I G R L V(18)
Ordering Information
Pierce GAPDH Antibody
Product #
PA1-988
Size
100 µg
Price
$230.00
Purchase
Add GAPDH Antibody to your cart.
Tested Applications Dilution *
Western Blot (WB) 1:500-1:5000
Published Applications Dilution
Western Blot (WB) See publications below
* Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own experiment using appropriate negative and positive controls.
Form Information
Form: Liquid
Concentration: 1mg/ml
Purification: Antigen affinity chromatography
Storage Buffer: PBS with 1mg/ml BSA
Preservative: 0.05% sodium azide
Storage Conditions: -20° C, Avoid Freeze/Thaw Cycles
Product Specific Information
PA1-988 detects rat GAPDH in human HeLa cell lysates and mouse and rat tissues..

PA1-988 has been used successfully in Western blotting. By Western blot this antibody specifically detects a ~36 kDa protein representing GAPDH.

PA1-988 immunizing peptide corresponds to amino acid residues 4-18 from human GAPDH.
General Information
Glyceraldehyde 3 phosphate dehydrogenase (GAPDH) is an enzyme involved in glycolysis. In addition to this role, it is involved in other cellular processes ranging from membrane fusion, neuronal apoptosis to cancer. GAPDH is expressed at high levels in most tissues and is therefore useful as protein loading control in western blot analysis. Additionally, the antigen is conserved in most eukaryotes.
Product Images
  • GAPDH Antibody (PA1-988) in WB
    PA1-988_1_07232010.jpg

    Western Blot with anti-GAPDH Polyclonal Antibody (PA1-988) (PA1-988_1_07232010.jpg)

    Western Blot with anti-GAPDH Polyclonal Antibody (PA1-988)

    Western blot of rat heart protein extract using PA1-988.
  • GAPDH Antibody (PA1-988) in WB
    PA1-988_WB_20131209144802.jpg

    Western Blot with anti-GAPDH Polyclonal Antibody (PA1-988) (PA1-988_WB_20131209144802.jpg)

    Western Blot with anti-GAPDH Polyclonal Antibody (PA1-988)

    Western blot analysis of GAPDH was performed by loading 25 ug of Hela (lane 1) and C6 (lane 2) cell lysates onto an SDS polyacrylamide gel. Proteins were transferred to a PVDF membrane and blocked at 4ºC overnight. The membrane was probed with a GAPDH polyclonal antibody (Product # PA1-988) at a dilution of 1:1000 overnight at 4°C, washed in TBST, and probed with an HRP-conjugated secondary antibody for 1 hr at room temperature in the dark. Chemiluminescent detection was performed using Pierce ECL Plus Western Blotting Substrate (Product # 32132). Results show a band at ~36 kDa.
Publications:
Western Blot
  Species / Dilution Summary
  Ms / 1:2,000
PA1-988 was used in western blot to study the effect of rimonabant on enzyme content of mouse 3T3 F442A preadipocytes

Mol Pharmacol. 2006 Feb;69(2):471-8.
"The cannabinoid CB1 receptor antagonist rimonabant (SR141716) inhibits cell proliferation and increases markers of adipocyte maturation in cultured mouse 3T3 F442A preadipocytes."
Author(s): Gary-Bobo M, Elachouri G, Scatton B, Le Fur G, Oury-Donat F, Bensaid M
Number of Citations: 1
(See PubMed article )
  Ms / Not Cited
PA1-988 was used in western blot to perform the global proteomics surveys of cardiac ventricle of a phospholamban mutant mouse

Mol Cell Proteomics. 2008 Mar;7(3):519-33.
"Comparative proteomics profiling of a phospholamban mutant mouse model of dilated cardiomyopathy reveals progressive intracellular stress responses."
Author(s): Gramolini AO, Kislinger T, Alikhani-Koopaei R, Fong V, Thompson NJ, Isserlin R, Sharma P, Oudit GY, Trivieri MG, Fagan A, Kannan A, Higgins DG, Huedig H, Hess G, Arab S, Seidman JG, Seidman CE, Frey B, Perry M, Backx PH, Liu PP, MacLennan DH, Emili A
Number of Citations: 1
(See PubMed article )
(This product is for In Vitro experimental use only. Not for resale without express authorization.)
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