G Protein alpha Inhibitor 1 + 2 Antibody

G Protein alpha Inhibitor 1 + 2 Polyclonal Antibody for Western blot, IP

Gnai1, GNAI2, Guanine Nucleotide Regulatory Protein Alpha 1/2, Adenylate cyclase-inhibiting G alpha protein, Guanine nucleotide-binding protein G(i) subunit alpha-1, Guanine nucleotide-binding protein G(i) subunit alpha-2
Host / Isotype: Rabbit / IgG
Class: Polyclonal
Type: Antibody
Tested Species Reactivity: Human (Hu), Mouse (Ms), Rat (Rt)
Published Species Reactivity: Human (Hu)
Immunogen: Synthetic peptide corresponding to residues K(346) N N L K D C G L F(355) of human G Protein alpha Inhibitor 1/2.
Ordering Information
Pierce G Protein alpha Inhibitor 1 + 2 Antibody
Product #
100 µg
Tested Applications Dilution *
Western Blot (WB) 1 µg/ml
Immunoprecipitation (IP) Assay dependent
Published Applications Dilution
Immunoprecipitation (IP) See publications below
* Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own experiment using appropriate negative and positive controls.
Form Information
Form: Liquid
Concentration: 1mg/ml
Purification: Antigen affinity chromatography
Storage Buffer: PBS with 1mg/ml BSA
Preservative: 0.05% sodium azide
Storage Conditions: -20° C, Avoid Freeze/Thaw Cycles
Product Specific Information
PA1-1000 detects G protein alpha inhibitor 1/2 from human, rat, and mouse tissues and cells.

PA1-1000 has been successfully used in Immunoprecipitation and Western blot procedures. By Western blot, this antibody detects an ~38 kDa protein representing G protein alpha inhibitor 1/2 from HeLa cell lysate.

The PA1-1000 immunogen is a synthetic peptide corresponding to residues K(346) N N L K D C G L F(355) of human G Protein alpha Inhibitor 1/2. This sequence is completely conserved in human, rat, guinea pig, hamster, bovine, hydra, octopus, Xenopus, chicken, canine, spiny dogfish, great pond snail, starfish, and American lobster G protein alpha inhibitor 1/2. PA1-1000 immunizing peptide (Cat. # PEP-129) is available for use in neutralization and control procedures.
General Information
The G protein family of signal transducers includes the alpha, beta, gamma subunits and the effector which are most clearly distinguished by their different alpha chains. The family also includes Gs and Gi, the stimulatory and inhibitory GTP-binding regulators of adenylate cyclase; Go, a protein of unknown function that is abundantly expressed in brain; and transducin 1 and 2, proteins involved in retinal phototransduction. The G proteins possess GTPase activity and are components of a complex membrane signaling system that includes membrane-bound receptors. The system exists to modulate extracellular signals as they are transmitted into the cell.
  Species / Dilution Summary
  Hu / Not Cited
PA1-1000 was used in immunoprecipitation to investigate the effects of gustducin and taste receptors on glucagon-like peptide-1

Proc Natl Acad Sci U S A. 2007 Sep 18;104(38):15069-74.
"Gut-expressed gustducin and taste receptors regulate secretion of glucagon-like peptide-1."
Author(s): Jang HJ, Kokrashvili Z, Theodorakis MJ, Carlson OD, Kim BJ, Zhou J, Kim HH, Xu X, Chan SL, Juhaszova M, Bernier M, Mosinger B, Margolskee RF, Egan JM
Number of Citations: 1
(See PubMed article )
(This product is for In Vitro experimental use only. Not for resale without express authorization.)
Part of Thermo Fisher Scientific