Flt3 / CD135 Antibody

Flt3 / CD135 Polyclonal Antibody for Western blot, IHC, FACS

>> See 10 other antibodies for FLT3 (Tyrosine-protein kinase receptor FLT3; STK1; FL cytokine receptor; Stem cell tyrosine kinase 1; CD135 antigen)
Synonyms:
Tyrosine-protein kinase receptor FLT3; STK1; FL cytokine receptor; Stem cell tyrosine kinase 1; CD135 antigen
Entrez Gene ID:
UniProt ID:
Details
Host / Isotype: Rabbit
Class: Polyclonal
Type: Antibody
Tested Species Reactivity: Human (Hu)
Immunogen: KLH conjugated synthetic peptide between 33-64 amino acids from the N-terminal region of human FLT3 (CD135)
Ordering Information
Pierce Flt3 / CD135 Antibody
Product #
PA5-14667
Size
400 ul
Price
$316.00
Purchase
Add Flt3 / CD135 Antibody to your cart.
Tested Applications Dilution *
Western Blot (WB) 1:1000
Immunohistochemistry (IHC) 1:50-1:100
Flow Cytometry (FACS) 1:10-1:50
* Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own experiment using appropriate negative and positive controls.
Form Information
Form: Liquid
Concentration: Lot-specific
Purification: Ammonium sulfate precipitation, Size-exclusion - Dialysis
Storage Buffer: PBS
Preservative: 0.09% sodium azide
Storage Conditions: Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C
Product Specific Information
General Information
Protein kinases are enzymes that transfer a phosphate group from a phosphate donor, generally the g phosphate of ATP, onto an acceptor amino acid in a substrate protein. By this basic mechanism, protein kinases mediate most of the signal transduction in eukaryotic cells, regulating cellular metabolism, transcription, cell cycle progression, cytoskeletal rearrangement and cell movement, apoptosis, and differentiation. With more than 500 gene products, the protein kinase family is one of the largest families of proteins in eukaryotes. The family has been classified in 8 major groups based on sequence comparison of their tyrosine (PTK) or serine/threonine (STK) kinase catalytic domains. The tyrosine kinase (TK) group is mainly involved in the regulation of cell-cell interactions such as differentiation, adhesion, motility and death. There are currently about 90 TK genes sequenced, 58 are of receptor protein TK (e.g. EGFR, EPH, FGFR, PDGFR, TRK, and VEGFR families), and 32 of cytosolic TK (e.g. ABL, FAK, JAK, and SRC families).
Product Images
  • Flt3 / CD135 Antibody (PA5-14667) in WB
    FLT3_Antibody_PA5-14667_WB_1_112013.jpg

    Western Blot with anti-Flt3  /  CD135 Polyclonal Antibody (PA5-14667) (FLT3_Antibody_PA5-14667_WB_1_112013.jpg)

    Western Blot with anti-Flt3 / CD135 Polyclonal Antibody (PA5-14667)

    Western blot analysis using a FLT3 polyclonal antibody (Product # PA5-14667) in HL-60 cell lysate. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence.
  • Flt3 / CD135 Antibody (PA5-14667) in IHC
    FLT3_Antibody_PA5-14667_IHC_1_112013.jpg

    Immunohistochemistry with anti-Flt3  /  CD135 Polyclonal Antibody (PA5-14667) (FLT3_Antibody_PA5-14667_IHC_1_112013.jpg)

    Immunohistochemistry with anti-Flt3 / CD135 Polyclonal Antibody (PA5-14667)

    Immunohistochemical analysis of formalin-fixed, paraffin-embedded human cancer tissue using a FLT3 polyclonal antibody (Product # PA5-14667), followed by HRP-conjugated secondary antibody and DAB staining.
  • Flt3 / CD135 Antibody (PA5-14667) in FACS
    FLT3_Antibody_PA5-14667_FC_1_112013.jpg

    Flow Cytometry with anti-Flt3  /  CD135 Polyclonal Antibody (PA5-14667) (FLT3_Antibody_PA5-14667_FC_1_112013.jpg)

    Flow Cytometry with anti-Flt3 / CD135 Polyclonal Antibody (PA5-14667)

    Flow cytometry analysis of 293 cells using a FLT3 polyclonal antibody (Product # PA5-14667) (right) compared to a negative control cell (left) at a dilution of 1:10-50, followed by a FITC-conjugated goat anti-rabbit antibody
(This product is for In Vitro experimental use only. Not for resale without express authorization.)
Part of Thermo Fisher Scientific