Flt3 / CD135 Antibody

Flt3 / CD135 Polyclonal Antibody for Western blot, IHC, FACS

>> See 10 other antibodies for FLT3 (Tyrosine-protein kinase receptor FLT3; STK1; FL cytokine receptor; Stem cell tyrosine kinase 1; CD135 antigen)
Synonyms:
Tyrosine-protein kinase receptor FLT3; STK1; FL cytokine receptor; Stem cell tyrosine kinase 1; CD135 antigen
Entrez Gene ID:
UniProt ID:
Details
Host / Isotype: Rabbit
Class: Polyclonal
Type: Antibody
Tested Species Reactivity: Human (Hu )
Immunogen: Synthetic peptide from the N-terminal region of FLT3 conjugated to KLH.
Ordering Information
Pierce Flt3 / CD135 Antibody
Product #
PA5-14667
Size
400 ul
Price
$316.00
Purchase
Add Flt3 / CD135 Antibody to your cart.
Tested Applications Dilution *
Western Blot (WB) 1:1000
Immunohistochemistry (IHC) 1:50-1:100
Flow Cytometry (FACS) 1:10 - 1:50
* Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own experiment using appropriate negative and positive controls.
Form Information
Form: Liquid
Concentration: Lot-specific
Purification: Protein G
Storage Buffer: PBS
Preservative: 0.09% sodium azide
Storage Conditions: Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C
Product Specific Information
PA5-14667 targets FLT3 in flow cytometry applications and shows reactivity with Human samples.

The PA5-14667 immunogen is a synthetic peptide from the N-terminal region of FLT3 conjugated to KLH.

PA5-14667 detects FLT3 which has a predicted molecular weight of approximately 113 kDa.
General Information
This gene encodes a class III receptor tyrosine kinase that regulates hematopoiesis. The receptor consists of an extracellular domain composed of five immunoglobulin-like domains, one transmembrane region, and a cytoplasmic kinase domain split into two parts by a kinase-insert domain. The receptor is activated by binding of the fms-related tyrosine kinase 3 ligand to the extracellular domain, which induces homodimer formation in the plasma membrane leading to autophosphorylation of the receptor. The activated receptor kinase subsequently phosphorylates and activates multiple cytoplasmic effector molecules in pathways involved in apoptosis, proliferation, and differentiation of hematopoietic cells in bone marrow. Mutations that result in the constitutive activation of this receptor result in acute myeloid leukemia and acute lymphoblastic leukemia.
Product Images
  • Flt3 / CD135 Antibody (PA5-14667) in WB
    FLT3_Antibody_PA5-14667_WB_1_112013.jpg

    Western Blot with anti-Flt3  /  CD135 Polyclonal Antibody (PA5-14667) (FLT3_Antibody_PA5-14667_WB_1_112013.jpg)

    Western Blot with anti-Flt3 / CD135 Polyclonal Antibody (PA5-14667)

    Western blot analysis of anti-FLT3 Polyclonal Antibody (PA5-14667) in HL-60 cell lysate. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence.
  • Flt3 / CD135 Antibody (PA5-14667) in IHC
    FLT3_Antibody_PA5-14667_IHC_1_112013.jpg

    Immunohistochemistry with anti-Flt3  /  CD135 Polyclonal Antibody (PA5-14667) (FLT3_Antibody_PA5-14667_IHC_1_112013.jpg)

    Immunohistochemistry with anti-Flt3 / CD135 Polyclonal Antibody (PA5-14667)

    Immunohistochemical staining of Formalin-fixed and paraffin-embedded human cancer tissue reacted with anti-FLT3 Polyclonal Antibody (PA5-14667), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
  • Flt3 / CD135 Antibody (PA5-14667) in FACS
    FLT3_Antibody_PA5-14667_FC_1_112013.jpg

    Flow Cytometry with anti-Flt3  /  CD135 Polyclonal Antibody (PA5-14667) (FLT3_Antibody_PA5-14667_FC_1_112013.jpg)

    Flow Cytometry with anti-Flt3 / CD135 Polyclonal Antibody (PA5-14667)

    Flow cytometric analysis of 293 cells using anti-FLT3 Polyclonal Antibody (PA5-14667) (right) compared to a negative control cell (left) at a dilution of 1:10-50. Secondary antibody used to visualize was a Goat Anti-Rabbit Secondary Antibody Conjugated to FITC.
(This product is for In Vitro experimental use only. Not for resale without express authorization.)
Part of Thermo Fisher Scientific