* Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own experiment using appropriate negative and positive controls.
0.09% sodium azide
Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C
Product Specific Information
PA5-14667 targets FLT3 in flow cytometry applications and shows reactivity with Human samples.
The PA5-14667 immunogen is a synthetic peptide from the N-terminal region of FLT3 conjugated to KLH.
PA5-14667 detects FLT3 which has a predicted molecular weight of approximately 113 kDa.
This gene encodes a class III receptor tyrosine kinase that regulates hematopoiesis. The receptor consists of an extracellular domain composed of five immunoglobulin-like domains, one transmembrane region, and a cytoplasmic kinase domain split into two parts by a kinase-insert domain. The receptor is activated by binding of the fms-related tyrosine kinase 3 ligand to the extracellular domain, which induces homodimer formation in the plasma membrane leading to autophosphorylation of the receptor. The activated receptor kinase subsequently phosphorylates and activates multiple cytoplasmic effector molecules in pathways involved in apoptosis, proliferation, and differentiation of hematopoietic cells in bone marrow. Mutations that result in the constitutive activation of this receptor result in acute myeloid leukemia and acute lymphoblastic leukemia.
Flt3 / CD135 Antibody (PA5-14667) in WB FLT3_Antibody_PA5-14667_WB_1_112013.jpg
Western Blot with anti-Flt3 / CD135 Polyclonal Antibody (PA5-14667)
Immunohistochemical staining of Formalin-fixed and paraffin-embedded human cancer tissue reacted with anti-FLT3 Polyclonal Antibody (PA5-14667), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
Flt3 / CD135 Antibody (PA5-14667) in FACS FLT3_Antibody_PA5-14667_FC_1_112013.jpg
Flow Cytometry with anti-Flt3 / CD135 Polyclonal Antibody (PA5-14667)
Flow cytometric analysis of 293 cells using anti-FLT3 Polyclonal Antibody (PA5-14667) (right) compared to a negative control cell (left) at a dilution of 1:10-50. Secondary antibody used to visualize was a Goat Anti-Rabbit Secondary Antibody Conjugated to FITC.
(This product is for In Vitro experimental use only. Not for resale without express authorization.)