Part of Thermo Fisher Scientific

Fibronectin Monoclonal Antibody (A35)

Fibronectin Antibody for ELISA, IHC, IP, Western blot

Synonyms:
FN, FN1, CIG, Cold insoluble globulin, fibronectin 1, LETS, MSF, Transformation sensitive protein
Entrez Gene ID:
(Human) 2335
UniProt ID:
P02751
Details
Host / Isotype: Mouse / IgG1, kappa
Class: Monoclonal
Type: Antibody
Clone: A35
Species Reactivity: Human (Hu)
Immunogen: Lysed bovine corneal endothelial cells and extracellular matrix.
Ordering Information
Pierce Fibronectin Monoclonal Antibody (A35)
Product Number Pkg. Size Price Purchase
CSI 005-35-02 200 µg $408.00
Add Fibronectin Monoclonal Antibody (A35) to your cart.


Storage: 4º C, store in dark
Form: 200 µg of Protein A/G purified antibody (1 mg/ml) in 0.01 M phosphate buffer, with 0.5 M NaCl and 15 mM sodium azide; pH 7.4.


Applications Dilution *
ELISA (ELISA) 1:4,000
Western Blot (WB) 1:100
Immunoprecipitation (IP) Assay dependent
Immunohistochemistry (IHC) Assay dependent
* Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own experiment using appropriate negative and positive controls.
Product Specific Information
CSI 005-35-02 is highly specific for fibronectin. There is no evidence for cross-reactivity with other connective tissue
protein (vitronectin, elastin, collagen, laminin). CSI 005-35-02 cross-reacts with human fibronectin. Other species have not been tested.

CSI 005-35-02 has been successfully used in Western blotting, ELISA, Immunohistochemistry and Immunoprecipitation procedures. The antibody can be used to probe fibronectin conformation and to quantitate plasma fibronectin in a sandwich ELISA with antibody CSI 005-32-02.

 The CSI 005-35-02 immunogen is lysed bovine corneal endothelial cells and extracelluar matrix.

The specificity of the CSI 005-35-02 epitope is located in the 40kDa Hep II heparin-binding domain, but differs from that of CSI 005-32-02.
General Information
Fibronectin is an adhesive glycoprotein with a molecular mass of 440 kDa. It is believed to be important for the formation of a provisional matrix that promotes cell adhesion and migration during wound healing. Its age-dependent increase in plasma and tissues may be accompanied in pathological states, especially in tumor growth, by its proteolytic breakdown by a number of neutral proteases. It has also shown that several of its proteolytic breakdown products exhibit unexpected and mostly harmful biological activities.
(This product is for In Vitro experimental use only. Not for resale without express authorization.)