Part of Thermo Fisher Scientific

Fibrillarin Monoclonal Antibody (38F3)

Fibrillarin Antibody for ICC, IF, IHC (P), Western blot

Synonyms:
FIB, FLRN, RNU3IP1
Entrez Gene ID:
(Human) 2091
UniProt ID:
P22087
Details
Host / Isotype: Mouse / IgG1
Class: Monoclonal
Type: Antibody
Clone: 38F3
Species Reactivity: Human (Hu) C. elegans (Ce) Plant (Pl) Rat (Rt) Drosophila (Dm) Yeast (Ys)
Immunogen: Yeast nuclear preparation (S. cerevisiae)
Ordering Information
Pierce Fibrillarin Monoclonal Antibody (38F3)
Product Number Pkg. Size Price Purchase
MA3-16771 500 µl $349.00
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Storage: Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C
Form: 500 µl of sterile-filtered tissue culture supernatant with 10mM sodium azide


Applications Dilution *
Immunohistochemistry (Paraffin) (IHC (P)) Assay Dependent
Western Blot (WB) Assay Dependent
Immunocytochemistry (ICC) Assay Dependent
Immunofluorescence (IF) Assay Dependent
* Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own experiment using appropriate negative and positive controls.
Product Specific Information
MA3-16771 detects Fibrillarin in Human, Rat, C. elegans, Drosophila, Plant and Yeast samples.

MA3-16771 has successfully been used in Immunocytochemistry, Immunofluorescence, Immunohistochemistry (Paraffin-embedded Sections) and Western Blot applications. The Fibrillarin molecular weight is 34 kDa. It is suggested the user determines the optimal dilution; however it has been found that MA3-16771 is useful in immunofluorescence at 1:1000-1:5000 (yeast cells), 1:500 (mammalian cells); Western blot 1:2000 (cell lysate), 1:10000 (nuclear fractions, ECL), 1:500 (mammalian fibrillarin, ECL), 1:1000-1:5000 (non-ECL).

The MA3-16771 immunogen is S. cerevisiae nuclear preparation.
General Information
Nop1p / Fibrillarin was originally identified as a nucleolar protein of bakers yeast, Saccharomyces cerevisiae (accession P15646). The Nop1p protein is essential for yeast viability and is localized in the nucleoli. The human homologue of Nop1p is fibrillarin (accession P22087) a component of the nucleolar small nuclear ribonucleoprotein (snRNP) particle. The human fibrillarin gene is located on chromosome 19 (19q13.1). Fibrillarin proteins have been cloned and sequenced from several other species (Mouse, accession P35550, Xenopus accession P22232, C. elegans accession Q22053, and S. pombe accession P35551). The N terminal 80 amino acids contain multiple copies based on the peptide RGG, and the remaining 240 amino acids consist of the fibrillarin domain. A fibrillarin homologue has also been identified in the genome of the archean Methanococcus (accession NC_000909). This protein lacks the RGG rich N-terminal extension but is clearly homologues to the other sequences throughout all of the fibrillarin domain. The structure of this molecule has been determined and shown to consist of 2 extended b-sheets flanked by 4 a-helixes. Patients with the autoimmune disease scleroderma often have strong circulating autoantibodies to a 34kDa protein which was subsequently found to be fibrillarin. Fibrillarin is an excellent marker for the nucleolus.
Product Images
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  • IHC using MA3-16771
    Immunohistochemistry with anti-Fibrillarin Monoclonal Antibody [38F3] (MA3-16771)

    Immunohistochemistry with anti-Fibrillarin Monoclonal Antibody [38F3] (MA3-16771)

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    Human SH-SY5Y cells stained with MA3-16771, showing prominent specular nucleolar staining. The nuclei are counter stained with blue DAPI DNA stain, so these spots appear very pale blue.
     
  • WB using MA3-16771
    Western Blot with anti-Fibrillarin Monoclonal Antibody [38F3] (MA3-16771)

    Western Blot with anti-Fibrillarin Monoclonal Antibody [38F3] (MA3-16771)

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    Strip blots of yeast protein extracts stained with the indicated antibodies; MA3-16771 is first lane on the left and stains a single band at ~34kDa.
     
 
 
(This product is for In Vitro experimental use only. Not for resale without express authorization.)