Fibrillarin Antibody (38F3)

Fibrillarin Monoclonal Antibody for Western blot, IF, ICC, IHC (F), IHC (P), FACS

Host / Isotype: Mouse / IgG1
Class: Monoclonal
Type: Antibody
Clone: 38F3
Tested Species Reactivity: Human (Hu), Mouse (Ms), Rat (Rt), C. elegans (Ce), Chicken (Ck), Drosophila (Dm), Plant (Pl), Yeast (Ys)
Published Species Reactivity: Plant (Pl)
Immunogen: Yeast nuclear preparation (S. cerevisiae)
Ordering Information
Pierce Fibrillarin Antibody (38F3)
Product #
250 µL
Tested Applications Dilution *
Western Blot (WB) 1:1000-1:5000
Immunofluorescence (IF) 1:500-1:1000
Immunocytochemistry (ICC) 1:500-1:1000
Immunohistochemistry (Frozen) (IHC (F)) 1:500-1:1000
Immunohistochemistry (Paraffin) (IHC (P)) 1:500-1:1000
Flow Cytometry (FACS) 1:20-1:400
Published Applications Dilution
Immunohistochemistry (IHC) See publications below
* Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own experiment using appropriate negative and positive controls.
Form Information
Form: Liquid
Storage Buffer: tissue culture supernatant
Preservative: 10mM sodium azide
Storage Conditions: Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Product Specific Information
Suggested positive control: antigen standard for FBL (transient overexpression lysate).
General Information
Nop1p / Fibrillarin was originally identified as a nucleolar protein of bakers yeast, Saccharomyces cerevisiae (accession P15646). The Nop1p protein is essential for yeast viability and is localized in the nucleoli. The human homologue of Nop1p is fibrillarin (accession P22087) a component of the nucleolar small nuclear ribonucleoprotein (snRNP) particle. The human fibrillarin gene is located on chromosome 19 (19q13.1). Fibrillarin proteins have been cloned and sequenced from several other species (Mouse, accession P35550, Xenopus accession P22232, C. elegans accession Q22053, and S. pombe accession P35551). The N terminal 80 amino acids contain multiple copies based on the peptide RGG, and the remaining 240 amino acids consist of the fibrillarin domain. A fibrillarin homologue has also been identified in the genome of the archean Methanococcus (accession NC_000909). This protein lacks the RGG rich N-terminal extension but is clearly homologues to the other sequences throughout all of the fibrillarin domain. The structure of this molecule has been determined and shown to consist of 2 extended b-sheets flanked by 4 a-helixes. Patients with the autoimmune disease scleroderma often have strong circulating autoantibodies to a 34kDa protein which was subsequently found to be fibrillarin. Fibrillarin is an excellent marker for the nucleolus.
Product Images
  • Fibrillarin Antibody (MA3-16771) in WB

    Western Blot with anti-Fibrillarin Monoclonal Antibody [38F3] (MA3-16771) (MA3-16771_WesternBlot.jpg)

    Western Blot with anti-Fibrillarin Monoclonal Antibody [38F3] (MA3-16771)

    Strip blots of yeast protein extracts stained with the indicated antibodies; MA3-16771 is first lane on the left and stains a single band at ~34kDa.
  • Fibrillarin Antibody (MA3-16771) in IF

    Immunofluorescence with anti-Fibrillarin Monoclonal Antibody [38F3] (MA3-16771) (MA3-16771_Immunohistochemistry.jpg)

    Immunofluorescence with anti-Fibrillarin Monoclonal Antibody [38F3] (MA3-16771)

    Human SH-SY5Y cells stained with MA3-16771, showing prominent specular nucleolar staining. The nuclei are counter stained with blue DAPI DNA stain, so these spots appear very pale blue.
  Species / Dilution Summary
  Pl / Not Cited
MA3-16771 was used in immunohistochemistry to study the roles of 5 potential co-factors of Arabidopsis ribosome biogenesis

PLoS One. 2013;8(1):e54084.
"40S ribosome biogenesis co-factors are essential for gametophyte and embryo development."
Author(s): Missbach S, Weis BL, Martin R, Simm S, Bohnsack MT, Schleiff E
Number of Citations: 1
(See PubMed article )
(This product is for In Vitro experimental use only. Not for resale without express authorization.)
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