Fibrillarin Antibody (38F3)

Fibrillarin Monoclonal Antibody for Western blot, IF, ICC, IHC (P)

Host / Isotype: Mouse / IgG1
Class: Monoclonal
Type: Antibody
Clone: 38F3
Tested Species Reactivity: Human (Hu), Mouse (Ms), Rat (Rt), C. elegans (Ce), Drosophila (Dm), Plant (Pl), Yeast (Ys)
Published Species Reactivity: Plant (Pl)
Immunogen: Yeast nuclear preparation (S cerevisiae)Hybridomas were screened by immunofluorescence on yeast cells and by western blotting on yeast protein homogenates (S cerevisiae)
Ordering Information
Pierce Fibrillarin Antibody (38F3)
Product #
250 µL
Tested Applications Dilution *
Western Blot (WB) 1:1000-1:2000
Immunofluorescence (IF) 1:500-1:5000
Immunocytochemistry (ICC) 1:500-1:5000
Immunohistochemistry (Paraffin) (IHC (P)) Assay dependent
Published Applications Dilution
Immunohistochemistry (IHC) See publications below
* Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own experiment using appropriate negative and positive controls.
Form Information
Form: Liquid
Storage Buffer: tissue culture supernatant
Preservative: 10mM sodium azide
Storage Conditions: -20° C, Avoid Freeze/Thaw Cycles
Product Specific Information
MA1-22000 detects fibrillarin in human, mouse, rat, plants, S. pombe, C. elegans and drosophila samples.

MA1-22000 has been successfully used in immunocytochemistry, immunofluorescence, immunohistochemistry (paraffin), and Western blot procedures.

The MA1-22000 immunogen is a yeast nuclear preparation (S cerevisiae). Hybridomas were screened by immunofluorescence on yeast cells and by western blotting on yeast protein homogenates (S cerevisiae).
General Information
Nop1p / Fibrillarin was originally identified as a nucleolar protein of bakers yeast, Saccharomyces cerevisiae (accession P15646). The Nop1p protein is essential for yeast viability and is localized in the nucleoli. The human homologue of Nop1p is fibrillarin (accession P22087) a component of the nucleolar small nuclear ribonucleoprotein (snRNP) particle. The human fibrillarin gene is located on chromosome 19 (19q13.1).
  Species / Dilution Summary
  Pl / Not Cited
MA1-22000 was used in immunohistochemistry to study the roles of 5 potential co-factors of Arabidopsis ribosome biogenesis

PLoS One. 2013;8(1):e54084.
"40S ribosome biogenesis co-factors are essential for gametophyte and embryo development."
Author(s): Missbach S, Weis BL, Martin R, Simm S, Bohnsack MT, Schleiff E
Number of Citations: 1
(See PubMed article )
(This product is for In Vitro experimental use only. Not for resale without express authorization.)
Part of Thermo Fisher Scientific