* Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own experiment using appropriate negative and positive controls.
tissue culture supernatant
10mM sodium azide
-20° C, Avoid Freeze/Thaw Cycles
Product Specific Information
MA1-22000 detects fibrillarin in human, mouse, rat, plants, S. pombe, C. elegans and drosophila samples.
MA1-22000 has been successfully used in immunocytochemistry, immunofluorescence, immunohistochemistry (paraffin), and Western blot procedures.
The MA1-22000 immunogen is a yeast nuclear preparation (S cerevisiae). Hybridomas were screened by immunofluorescence on yeast cells and by western blotting on yeast protein homogenates (S cerevisiae).
Nop1p / Fibrillarin was originally identified as a nucleolar protein of bakers yeast, Saccharomyces cerevisiae (accession P15646). The Nop1p protein is essential for yeast viability and is localized in the nucleoli. The human homologue of Nop1p is fibrillarin (accession P22087) a component of the nucleolar small nuclear ribonucleoprotein (snRNP) particle. The human fibrillarin gene is located on chromosome 19 (19q13.1).
Fibrillarin Antibody (MA1-22000) in WB MA1-22000_WB.jpg
Western Blot with anti-Fibrillarin Monoclonal Antibody [38F3] (MA1-22000)
Immunofluorescent analysis of Fibrillarin in SH-SY5Y cells using a Fibrillarin monoclonal antibody (Product # MA1-22000) (green) at a dilution of 1:100,000 and with a chicken antibody to neurofilament NF-H (red) at a dilution of 1:1000. Nuclei were counterstained with Hoechst (blue).
Fibrillarin Antibody (MA1-22000) in IF MA1-22000_IF_2.jpg
Immunofluorescence with anti-Fibrillarin Monoclonal Antibody [38F3] (MA1-22000)
Immunofluorescent analysis of Fibrillarin in rat neurons and glia using a Fibrillarin monoclonal antibody (Product # MA1-22000) (green) and with a chicken antibody to neurofilament NF-H (red). Nuclei were counterstained using Hoechst (blue).
MA1-22000 was used in immunohistochemistry to study the roles of 5 potential co-factors of Arabidopsis ribosome biogenesis
PLoS One. 2013;8(1):e54084. "40S ribosome biogenesis co-factors are essential for gametophyte and embryo development."
Author(s): Missbach S, Weis BL, Martin R, Simm S, Bohnsack MT, Schleiff E
Number of Citations: 1
(See PubMed article )
(This product is for In Vitro experimental use only. Not for resale without express authorization.)