Part of Thermo Fisher Scientific

Fas Ligand (human) Recombinant Protein

Fas Ligand (human) Protein for Ctrl, ELISA, FACS, Western blot

Synonyms:
FasL, apoptosis antigen ligand 1, CD95 ligand, CD95L, CD178, APO1
Details
Class: Recombinant
Type: Protein
Species Reactivity: Not Applicable (N/A)
Ordering Information
Pierce Fas Ligand (human) Recombinant Protein
Product Number Pkg. Size Price Purchase
RP-808 5 µg $284.00
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Storage: -20º C, Avoid Freeze/Thaw Cycles
Form: 5 µg of purified (>95%), E. coli expressed, recombinant protein (1mg/ml) in 8 M Urea, 100 mM NaH2PO4, and 10 mM Tris pH 8.0.


Applications Dilution *
ELISA (ELISA) Assay dependent
Western Blot (WB) Assay dependent
Flow Cytometry (FACS) Assay dependent
Control (Ctrl) Assay Dependent
* Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own experiment using appropriate negative and positive controls.
Product Specific Information
This human recombinant protein was produced in E. coli.

RP-808 can be used as a positive control in Western blots with ABR polyclonal Fas Ligand antibody (MA1-4803 and MA1-4804), as well as in ELISA and flow cytometry applications.

RP-808 calculated molecular weight is ~26 kDa.

RP-808 is a recombinant protein (aa 95-280) with N-terminal (His) 10-tag.
General Information
Fas ligand (FasL, CD 95L) is a type-II-membrane protein, whose N-terminus is in the cytoplasm and its C-terminal region extends into the extracellular space. Its receptor Fas (CD 95, Apo-1) is a cell-surface-type-I-membrane protein and a member of the tumor necrosis factor (TNF) and nerve growth factor (NGF) receptor family.
As a member of the TNF-cytokine family FasL induces apoptosis when interacting with its receptor Fas. FasL may exist as either membrane bound (45 kD) or soluble forms (26 kD).
The soluble protein can be released from cells upon cleavage by metalloproteinases. Binding of FasL to Fas leads to oligomerization of the receptor and triggers apoptotic cell death through the interaction of other proteins.
FasL is predominantly expressed in activated T-lymphocytes and natural killer (NK) cells also it is expressed in the tissues of immune-privilege sites such as testis and eye. FasL expression is also reported in various tissues as thymus, liver, ovary, lung, heart and kidney.
The Fas/FasL system has been shown to play a role in a number of human diseases, for example AIDS, hepatitis or cancer. It is assumed that induction of apoptosis through FasL is predominantly involved in anti-viral immune responses.
(This product is for In Vitro experimental use only. Not for resale without express authorization.)