FLAG™ Epitope Tag (DYKDDDDK) Antibody (FG4R)

FLAG™ Epitope Tag (DYKDDDDK) Monoclonal Antibody for Western blot, IF, IP

Details
Host / Isotype: Mouse / IgG2b
Class: Monoclonal
Type: Antibody
Clone: FG4R
Tested Species Reactivity: Tag (Tag)
Published Species Reactivity: Not Applicable (N/A)
Immunogen: Synthetic peptide (DYKDDDDK) coupled to KLH.
Ordering Information
Pierce FLAG™ Epitope Tag (DYKDDDDK) Antibody (FG4R)
Product #
MA1-91878
Size
100 µg
Price
$149.00
Purchase
Add FLAG™ Epitope Tag (DYKDDDDK) Antibody (FG4R) to your cart.
Tested Applications Dilution *
Western Blot (WB) 1:1000
Immunofluorescence (IF) 1:200
Immunoprecipitation (IP) 1:200
Published Applications Dilution
Western Blot (WB) See publications below
Immunocytochemistry (ICC) See publications below
Immunoprecipitation (IP) See publications below
* Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own experiment using appropriate negative and positive controls.
Form Information
Form: Liquid
Concentration: 1mg/ml
Purification: Protein A
Storage Buffer: PBS, pH 7.2
Preservative: no preservative
Storage Conditions: Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Product Specific Information
MA1-91878 detects Flag tag from various samples.

MA1-91878 has been successfully used in immunoprecipitation, Western blot and immunofluorescence applications.

The MA1-91878 immunogen is synthetic peptide (DYKDDDDK) coupled to KLH.

MA1-91878 may be stored at 4ºC short term. Add 0.05% sodium azide if desired. For long term storage, store at -20ºC, avoiding freeze/thaw cycles.
General Information
Epitope tags provide a method to localize gene products in a variety of cell types, study the topology of proteins and protein complexes, identify associated proteins, and characterize newly identified, low abundance or poorly immunogenic proteins when protein specific antibodies are not available. Tagging with xxxDDDDK may be done at the N-terminus, N-terminus preceded by a methionine residue, C-terminus, and in internal positions of the target protein. The small size of the epitope tag and its high hydrophilicity tend to decrease the possibility of interference with protein expression, proteolytic maturation, antigenicity and function. The enterokinase cleavage site allows it to be completely removed from the purified fusion proteins.
Product Images
  • FLAG™ Epitope Tag (DYKDDDDK) Antibody (MA1-91878) in WB
    MA1-91878_WesternBlot.jpg

    Western Blot with anti-FLAG™ Epitope Tag (DYKDDDDK) Monoclonal Antibody [FG4R] (MA1-91878) (MA1-91878_WesternBlot.jpg)

    Western Blot with anti-FLAG™ Epitope Tag (DYKDDDDK) Monoclonal Antibody [FG4R] (MA1-91878)

    1:1000 (1ug/mL) Ab dilution probed against HEK293 cells transfected with DYKDDDDK-tagged protein vector; untransfected (1) and transfected (2)
Publications:
Western Blot
  Species / Dilution Summary
  N/A / 0
MA1-91878 was used in western blot to elucidate the signaling pathways that control tumor angiogenesis

Cancer Res. 2001 Dec 1;61(23):8416-21.
"The constitutive photomorphogenesis 9 signalosome directs vascular endothelial growth factor production in tumor cells."
Author(s): Pollmann C, Huang X, Mall J, Bech-Otschir D, Naumann M, Dubiel W
Number of Citations: 10
(See PubMed article )
  N/A / 0
MA1-91878 was used in western blot to generate small-molecule inhibitors of the protease NS3

J Biol Chem. 2004 Mar 12;279(11):10222-7.
"Serpin mechanism of hepatitis C virus nonstructural 3 (NS3) protease inhibition: induced fit as a mechanism for narrow specificity."
Author(s): Richer MJ, Juliano L, Hashimoto C, Jean F
Number of Citations: 3
(See PubMed article )
  N/A / 0
MA1-91878 was used in western blot to investigate how BMP-7 opposes TGF-beta signaling in mesangial cells

J Biol Chem. 2004 May 28;279(22):23200-6.
"Bone morphogenetic protein-7 signals opposing transforming growth factor beta in mesangial cells."
Author(s): Wang S, Hirschberg R
Number of Citations: 12
(See PubMed article )
  N/A / 0
MA1-91878 was used in western blot to identify modulators regulated by human papillomavirus 16 E7 oncogene

Gynecol Oncol. 2005 Oct;99(1):142-52.
"Protein profiling and identification of modulators regulated by human papillomavirus 16 E7 oncogene in HaCaT keratinocytes by proteomics."
Author(s): Lee KA, Kang JW, Shim JH, Kho CW, Park SG, Lee HG, Paik SG, Lim JS, Yoon DY
Number of Citations: 6
(See PubMed article )
  N/A / 0
MA1-91878 was used in western blot to study mechanisms of menadione-induced cytotoxicity

Biochem Biophys Res Commun. 2005 Oct 21;336(2):565-71.
"Role of a novel dual flavin reductase (NR1) and an associated histidine triad protein (DCS-1) in menadione-induced cytotoxicity."
Author(s): Kwa?nicka-Crawford DA, Vincent SR
Number of Citations: 1
(See PubMed article )
  N/A / 0
MA1-91878 was used in western blot to purify and characterize GINS from Saccharomyces cerevisiae

J Biol Chem. 2006 Jul 28;281(30):21422-32.
"GINS is a DNA polymerase epsilon accessory factor during chromosomal DNA replication in budding yeast."
Author(s): Seki T, Akita M, Kamimura Y, Muramatsu S, Araki H, Sugino A
Number of Citations: 8
(See PubMed article )
  N/A / 0
MA1-91878 was used in western blot to study the mechanisms underlying SRC-3/AIB1 coactivator complex disassembly

Mol Cell Biol. 2006 Nov;26(21):7846-57.
"Signaling within a coactivator complex: methylation of SRC-3/AIB1 is a molecular switch for complex disassembly."
Author(s): Feng Q, Yi P, Wong J, O'Malley BW
Number of Citations: 51
(See PubMed article )
  N/A / 1:1000
MA1-91878 was used in western blot to determine whether human guanylate binding protein-1 is secreted from cells

Am J Pathol. 2006 Sep;169(3):1088-99.
"Human guanylate binding protein-1 is a secreted GTPase present in increased concentrations in the cerebrospinal fluid of patients with bacterial meningitis."
Author(s): Naschberger E, Lubeseder-Martellato C, Meyer N, Gessner R, Kremmer E, Gessner A, Stürzl M
Number of Citations: 7
(See PubMed article )
  N/A / 0
MA1-91878 was used in western blot to examine the link between XAP2 and AHR transcriptional activity

Mol Pharmacol. 2006 Dec;70(6):2096-107.
"Endogenous hepatic expression of the hepatitis B virus X-associated protein 2 is adequate for maximal association with aryl hydrocarbon receptor-90-kDa heat shock protein complexes."
Author(s): Hollingshead BD, Patel RD, Perdew GH
Number of Citations: 5
(See PubMed article )
  N/A / 0
MA1-91878 was used in western blot to optimise expansion of genetically modified T cells using an antibody/interleukin-2 receptor chimera

J Immunol Methods. 2008 Aug 20;337(1):16-23.
"Selective expansion of genetically modified T cells using an antibody/interleukin-2 receptor chimera."
Author(s): Sogo T, Kawahara M, Tsumoto K, Kumagai I, Ueda H, Nagamune T
Number of Citations: 2
(See PubMed article )
  N/A / 0
MA1-91878 was used in immunoprecipitation and western blot to study deubiquitination of estrogen receptor alpha

J Biol Chem. 2009 Jun 12;284(24):16135-45.
"OTU Domain-containing ubiquitin aldehyde-binding protein 1 (OTUB1) deubiquitinates estrogen receptor (ER) alpha and affects ERalpha transcriptional activity."
Author(s): Stanisi? V, Malovannaya A, Qin J, Lonard DM, O'Malley BW
Number of Citations: 5
(See PubMed article )
Immunocytochemistry
  Species / Dilution Summary
  N/A / 0
MA1-91878 was used in immunocytochemistry to study mechanisms underlying myotube enlargement in Drosophila

J Cell Biol. 2005 Jun 20;169(6):909-20.
"A positive feedback loop between Dumbfounded and Rolling pebbles leads to myotube enlargement in Drosophila."
Author(s): Menon SD, Osman Z, Chenchill K, Chia W
Number of Citations: 20
(See PubMed article )
  N/A / 0
MA1-91878 was used in immunocytochemistry to determine the role of MAN1 in mouse development

Development. 2007 Apr;134(7):1385-95.
"The nuclear envelope protein MAN1 regulates TGFbeta signaling and vasculogenesis in the embryonic yolk sac."
Author(s): Cohen TV, Kosti O, Stewart CL
Number of Citations: 12
(See PubMed article )
Immunoprecipitation
  Species / Dilution Summary
  N/A / 0
MA1-91878 was used in immunoprecipitation to study the mechanisms by which phosphorylation affects Pin1 function

Mol Cell Biol. 2005 Nov;25(21):9687-99.
"Peptidyl-prolyl isomerase 1 (Pin1) serves as a coactivator of steroid receptor by regulating the activity of phosphorylated steroid receptor coactivator 3 (SRC-3/AIB1)."
Author(s): Yi P, Wu RC, Sandquist J, Wong J, Tsai SY, Tsai MJ, Means AR, O'Malley BW
Number of Citations: 37
(See PubMed article )
  N/A / 0
MA1-91878 was used in immunoprecipitation to investigate interactions between pyruvate kinase isoforms and hepatitis C virus NS5B

FEBS Lett. 2008 Jun 25;582(15):2155-60.
"Isoform-specific interaction of pyruvate kinase with hepatitis C virus NS5B."
Author(s): Wu X, Zhou Y, Zhang K, Liu Q, Guo D
Number of Citations: 4
(See PubMed article )
  N/A / 0
MA1-91878 was used in immunoprecipitation and western blot to study deubiquitination of estrogen receptor alpha

J Biol Chem. 2009 Jun 12;284(24):16135-45.
"OTU Domain-containing ubiquitin aldehyde-binding protein 1 (OTUB1) deubiquitinates estrogen receptor (ER) alpha and affects ERalpha transcriptional activity."
Author(s): Stanisi? V, Malovannaya A, Qin J, Lonard DM, O'Malley BW
Number of Citations: 5
(See PubMed article )
  N/A / 0
MA1-91878 was used in immunoprecipitation to study the effects of DISC1 polymorphisms on Wnt/GSK3? signaling and brain development

Neuron. 2011 Nov 17;72(4):545-58.
"Common DISC1 polymorphisms disrupt Wnt/GSK3? signaling and brain development."
Author(s): Singh KK, De Rienzo G, Drane L, Mao Y, Flood Z, Madison J, Ferreira M, Bergen S, King C, Sklar P, Sive H, Tsai LH
Number of Citations: 7
(See PubMed article )
(This product is for In Vitro experimental use only. Not for resale without express authorization.)
Part of Thermo Fisher Scientific