Part of Thermo Fisher Scientific

FAK-Focal Adhesion Kinase Polyclonal Antibody

FAK-Focal Adhesion Kinase Antibody for IF, Western blot

Synonyms:
FADK 1, FAK-related non-kinase polypeptide, focal adhesion kinase 1, protein-tyrosine kinase 2
Entrez Gene ID:
(Human) 5747
UniProt ID:
Q05397
Details
Host / Isotype: Rabbit / IgG
Class: Polyclonal
Type: Antibody
Species Reactivity: Human (Hu) Murine (Ms) Rat (Rt) Canine (Ca) Amphibian (Am)
Immunogen: Recombinant protein corresponding to aa 853-1052 from the C-terminus of human FAK
Ordering Information
Pierce FAK-Focal Adhesion Kinase Polyclonal Antibody
Product Number Pkg. Size Price Purchase
PA5-16676 500 ul $281.00
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Storage: 4º C, DO NOT FREEZE!
Form: 500 ul of total IgG purified from rabbit anti-serum by Protein A chromatography (1 mg/ml). Prepared at 1mg/mL in 10mM PBS, pH 7.4, with 0.2% BSA and 0.09% azide.


Applications Dilution *
Immunofluorescence (IF) Assay Dependent
Western Blot (WB) 5-10 µg/mL
* Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own experiment using appropriate negative and positive controls.
Product Specific Information
PA5-16676 targets FAK-Focal Adhesion Kinase in IF and WB applications and shows reactivity with Amphibian, Canine, Human, Murine, and Rat samples.

The PA5-16676 immunogen is recombinant protein corresponding to aa 853-1052 from the C-terminus of human FAK.
General Information
FAK (Focal Adhesion Associated Protein-Tyrosine Kinase) is a widely expressed non-receptor protein tyrosine kinase (PTK). FAK has a central catalytic domain and a C-terminal tail that localizes it to focal adhesions, which are sites where cells attach to the extracellular matrix via surface integrin receptors. Increased FAK tyrosine phosphorylation occurs upon integrin engagement with fibronectin. Adhesion of murine NIH3T3 fibroblasts to fibronectin promotes association of the Grb2 adapter protein and c-Src PTK with FAK in vivo, and also results in activation of the ERK2 MAP kinase. In v-Src-transformed NIH3T3, the association of v-Src, Grb2, and Sos with FAK is independent of cell adhesion to fibronectin. In vitro the Grb2 SH2 domain binds directly to tyrosine-phosphorylated FAK, and the binding site has been identified as Tyr925 by site directed mutagenesis.
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    Western Blot with anti-FAK-Focal Adhesion Kinase Polyclonal Antibody (PA5-16676)

    Western Blot with anti-FAK-Focal Adhesion Kinase Polyclonal Antibody (PA5-16676)

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(This product is for In Vitro experimental use only. Not for resale without express authorization.)