ErbB3 Antibody (RTJ2)

ErbB3 Monoclonal Antibody for Western blot, IF, IHC, IHC (P), FACS, IP, ELISA

>> See 22 other antibodies for ERBB3 (C-errB-3, ERBB3, MDA BF 1, p180 ErbB3, p45 sErbB3, p85 sErbB3, receptor tyrosine protein kinase ERB3, tyrosine kinase type cell surface receptor HER3,)
Synonyms:
C-errB-3, ERBB3, MDA BF 1, p180 ErbB3, p45 sErbB3, p85 sErbB3, receptor tyrosine protein kinase ERB3, tyrosine kinase type cell surface receptor HER3,
Entrez Gene ID:
UniProt ID:
Details
Host / Isotype: Mouse / IgG1
Class: Monoclonal
Type: Antibody
Clone: RTJ2
Tested Species Reactivity: Human (Hu), Mouse (Ms)
Published Species Reactivity: Human (Hu)
Immunogen: Synthetic Peptide: E(1004) L E P E L D L D L D L E(1016)
Ordering Information
Pierce ErbB3 Antibody (RTJ2)
Product #
MA1-860
Size
200 µg
Price
$316.00
Purchase
Add ErbB3 Antibody (RTJ2) to your cart.
Tested Applications Dilution *
Western Blot (WB) Assay Dependent
Immunofluorescence (IF) 10 µg/mL
Immunohistochemistry (IHC) 6 - 8 µg/mL
Immunohistochemistry (Paraffin) (IHC (P)) 5 µg/ml
Flow Cytometry (FACS) 0.5-2 ug per 10^6 cells
Immunoprecipitation (IP) Assay dependent
ELISA (ELISA) Assay dependent
Published Applications Dilution
Western Blot (WB) See publications below
* Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own experiment using appropriate negative and positive controls.
Form Information
Form: Liquid
Concentration: 1mg/ml
Purification: Protein G
Storage Buffer: PBS with 1mg/ml BSA
Preservative: 0.05% sodium azide
Storage Conditions: -20° C, Avoid Freeze/Thaw Cycles
Product Specific Information
MA1-860 detects Her-3 from human and mouse samples.

MA1-860 has been successfully used in FACS, immunoprecipitation, immunohistochemical, immunofluorescence and ELISA procedures. By immunoprecipitation, this antibody detects ~185 kDa protein, representing Her-3 from human tissue extracts.This antibody has also been used in immunohistochemical, immunofluorescence and ELISA procedures using human tissues.

MA1-860 immunizing peptide corresponds to amino acid residues 1004 to 1016 of human cytoplasmic domain of HER-3 protein.
General Information
Her-3, also known as c-erbB-3, is a Type 1 membrane protein found in numerous cell types. It is a member of the EGF receptor protein family, and is activated by neuregulins and NTAK. Her-3 levels have been found to be elevated in certain human tumor cell lines suggesting that it may play a role in some malignancies. Her-3 is a receptor for heregulin and is capable of mediating HGL-stimulated tyrosine phosphorylation of itself.
Product Images
  • ErbB3 Antibody (MA1-860) in FACS
    MA1-860_FACS_Hela_20130731112651.jpg

    Flow Cytometry with anti-ErbB3 Monoclonal Antibody [RTJ2] (MA1-860) (MA1-860_FACS_Hela_20130731112651.jpg)

    Flow Cytometry with anti-ErbB3 Monoclonal Antibody [RTJ2] (MA1-860)

    Flow cytometry analysis of ErbB3 in Hela cells (green) compared to an isotype control (blue). Cells were harvested, adjusted to a concentration of 1-5x10^6 cells/ml, fixed with 2% paraformaldehyde and washed with PBS. Cells were blocked with a 2% solution of BSA-PBS for 30 min at room temperature and incubated with a ErbB3 monoclonal antibody (Product # MA1-860) at a dilution of 0.5 ug/test for 60 min at room temperature. Cells were then incubated for 40 min at room temperature in the dark using a Dylight 488-conjugated goat anti-mouse IgG (H+L) secondary antibody and re-suspended in PBS for FACS analysis.
  • ErbB3 Antibody (MA1-860) in FACS
    MA1-860_FACS_MCF-7_20130731112709.jpg

    Flow Cytometry with anti-ErbB3 Monoclonal Antibody [RTJ2] (MA1-860) (MA1-860_FACS_MCF-7_20130731112709.jpg)

    Flow Cytometry with anti-ErbB3 Monoclonal Antibody [RTJ2] (MA1-860)

    Flow cytometry analysis of ErbB3 in MCF-7 cells (green) compared to an isotype control (blue). Cells were harvested, adjusted to a concentration of 1-5x10^6 cells/ml, fixed with 2% paraformaldehyde and washed with PBS. Cells were blocked with a 2% solution of BSA-PBS for 30 min at room temperature and incubated with a ErbB3 monoclonal antibody (Product # MA1-860) at a dilution of 0.5 ug/test for 60 min at room temperature. Cells were then incubated for 40 min at room temperature in the dark using a Dylight 488-conjugated goat anti-mouse IgG (H+L) secondary antibody and re-suspended in PBS for FACS analysis.
  • ErbB3 Antibody (MA1-860) in FACS
    MA1-860_FACS_Neuro-2a_20130731112727.jpg

    Flow Cytometry with anti-ErbB3 Monoclonal Antibody [RTJ2] (MA1-860) (MA1-860_FACS_Neuro-2a_20130731112727.jpg)

    Flow Cytometry with anti-ErbB3 Monoclonal Antibody [RTJ2] (MA1-860)

    Flow cytometry analysis of ErbB3 in Neuro-2a cells (green) compared to an isotype control (blue). Cells were harvested, adjusted to a concentration of 1-5x10^6 cells/ml, fixed with 2% paraformaldehyde and washed with PBS. Cells were blocked with a 2% solution of BSA-PBS for 30 min at room temperature and incubated with a ErbB3 monoclonal antibody (Product # MA1-860) at a dilution of 0.5 ug/test for 60 min at room temperature. Cells were then incubated for 40 min at room temperature in the dark using a Dylight 488-conjugated goat anti-mouse IgG (H+L) secondary antibody and re-suspended in PBS for FACS analysis.
Publications:
Western Blot
  Species / Dilution Summary
  Hu / 1:200
MA1-860 was used in western blot to investigate the characteristics of neuregulin in human ovarian cancer cells

Clin Cancer Res. 2002 Dec;8(12):3933-42.
"Neuregulin expression, function, and signaling in human ovarian cancer cells."
Author(s): Gilmour LM, Macleod KG, McCaig A, Sewell JM, Gullick WJ, Smyth JF, Langdon SP
Number of Citations: 12
(See PubMed article )
(This product is for In Vitro experimental use only. Not for resale without express authorization.)
Part of Thermo Fisher Scientific