Endostatin Antibody

Endostatin Polyclonal Antibody for Western blot, IF, ICC, IHC (P)

>> See 5 other antibodies for COL18A1 (Alpha 1 Collagen type 18 (XVIII)(COL18A1), COL18A1, Endostatin, KNO)
Synonyms:
Alpha 1 Collagen type 18 (XVIII)(COL18A1), COL18A1, Endostatin, KNO
Entrez Gene ID:
UniProt ID:
Details
Host / Isotype: Rabbit
Class: Polyclonal
Type: Antibody
Tested Species Reactivity: Human (Hu), Mouse (Ms)
Published Species Reactivity: Mouse (Ms), Non-human primate (Nhp)
Immunogen: Synthetic Peptide: R(129) R L M/T E S Y C E T W R T E(142)
Ordering Information
Pierce Endostatin Antibody
Product #
PA1-601
Size
100 µg
Price
$316.00
Purchase
Add Endostatin Antibody to your cart.
Tested Applications Dilution *
Western Blot (WB) 2 µg/ml
Immunofluorescence (IF) 1:200-1:1000
Immunocytochemistry (ICC) 1:200-1:1000
Immunohistochemistry (Paraffin) (IHC (P)) 1:100-1:500
Published Applications Dilution
Western Blot (WB) See publications below
Immunohistochemistry (IHC) See publications below
* Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own experiment using appropriate negative and positive controls.
Form Information
Form: Liquid
Concentration: 1mg/ml
Purification: Antigen affinity chromatography
Storage Buffer: PBS with 1mg/ml BSA
Preservative: 0.05% sodium azide
Storage Conditions: -20° C, Avoid Freeze/Thaw Cycles
Product Specific Information
PA1-601 detects recombinant human and mouse endostatin.

PA1-601 has been successfully used in Western blot, IF/ICC and IHC (P) procedures. By Western blot, this antibody detects a 20 kDa protein representing recombinant human endostatin.

PA1-601 immunizing peptides are two peptides derived from mouse and human endostatin protein corresponding to amino acid residues 129-142 of endostatin.
General Information
Endostatin is a 20-kDa proteolytic COOH-terminal fragment derived from collagen XVIII. Endostatin reduces endothelial cell proliferation and acts as a potent inhibitor of angiogenesis and tumor growth.
Product Images
  • Endostatin Antibody (PA1-601) in IF
    PA1-601_IF_NIH-3T3.jpg

    Immunofluorescence with anti-Endostatin Polyclonal Antibody (PA1-601) (PA1-601_IF_NIH-3T3.jpg)

    Immunofluorescence with anti-Endostatin Polyclonal Antibody (PA1-601)

    Immunofluorescent analysis of Endostatin (green) showing positive staining in the secretion of NIH-3T3 cells (right) compared with a negative control in the absence of primary antibody (left). Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes, blocked with 3% BSA-PBS for 30 minutes at room temperature and probed with an Endostatin polyclonal antibody (Product # PA1-601) in 3% BSA-PBS at a dilution of 1:200 and incubated overnight at 4 ºC in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 488-conjugated goat-anti-rabbit IgG secondary antibody in PBS at room temperature in the dark. F-actin (red) was stained with a flourescent red phalloidin and nuclei (blue) were stained with DAPI for 5-10 minutes in the dark. Images were taken at a magnification of 60x.
  • Endostatin Antibody (PA1-601) in IF
    PA1-601_IF_293T.jpg

    Immunofluorescence with anti-Endostatin Polyclonal Antibody (PA1-601) (PA1-601_IF_293T.jpg)

    Immunofluorescence with anti-Endostatin Polyclonal Antibody (PA1-601)

    Immunofluorescent analysis of Endostatin (green) showing positive staining in the secretion of 293T cells (right) compared with a negative control in the absence of primary antibody (left). Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes, blocked with 3% BSA-PBS for 30 minutes at room temperature and probed with an Endostatin polyclonal antibody (Product # PA1-601) in 3% BSA-PBS at a dilution of 1:200 and incubated overnight at 4 ºC in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 488-conjugated goat-anti-rabbit IgG secondary antibody in PBS at room temperature in the dark. F-actin (red) was stained with a flourescent red phalloidin and nuclei (blue) were stained with DAPI for 5-10 minutes in the dark. Images were taken at a magnification of 60x.
  • Endostatin Antibody (PA1-601) in IF
    PA1-601_IF_A431.jpg

    Immunofluorescence with anti-Endostatin Polyclonal Antibody (PA1-601) (PA1-601_IF_A431.jpg)

    Immunofluorescence with anti-Endostatin Polyclonal Antibody (PA1-601)

    Immunofluorescent analysis of Endostatin (green) showing positive staining in the secretion of A431 cells (right) compared with a negative control in the absence of primary antibody (left). Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes, blocked with 3% BSA-PBS for 30 minutes at room temperature and probed with an Endostatin polyclonal antibody (Product # PA1-601) in 3% BSA-PBS at a dilution of 1:200 and incubated overnight at 4 ºC in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 488-conjugated goat-anti-rabbit IgG secondary antibody in PBS at room temperature in the dark. F-actin (red) was stained with a flourescent red phalloidin and nuclei (blue) were stained with DAPI for 5-10 minutes in the dark. Images were taken at a magnification of 60x.
  • Endostatin Antibody (PA1-601) in IHC (P)
    PA1-601_IHC_human-kidney-tissue.jpg

    Immunohistochemistry (Paraffin) with anti-Endostatin Polyclonal Antibody (PA1-601) (PA1-601_IHC_human-kidney-tissue.jpg)

    Immunohistochemistry (Paraffin) with anti-Endostatin Polyclonal Antibody (PA1-601)

    Immunohistochemistry analysis of Endostatin showing positive staining in the secretion of paraffin-treated Human kidney tissue (right) compared with a negative control in the absence of primary antibody (left). To expose target proteins, antigen retrieval method was performed using 10mM sodium citrate (pH 6.0), microwaved for 8-15 min. Following antigen retrieval, tissues were blocked in 3% H2O2-methanol for 15 min at room temperature, washed with ddH2O and PBS, and then probed with an endostatin polyclonal antibody (Product # PA1-601) diluted by 3% BSA-PBS at a dilution of 1:100 overnight at 4°C in a humidified chamber. Tissues were washed extensively PBST and detection was performed using an HRP-conjugated secondary antibody followed by colorimetric detection using a DAB kit. Tissues were counterstained with hematoxylin and dehydrated with ethanol and xylene to prep for mounting.
  • Endostatin Antibody (PA1-601) in IHC (P)
    PA1-601_IHC_human-colon-tissue.jpg

    Immunohistochemistry (Paraffin) with anti-Endostatin Polyclonal Antibody (PA1-601) (PA1-601_IHC_human-colon-tissue.jpg)

    Immunohistochemistry (Paraffin) with anti-Endostatin Polyclonal Antibody (PA1-601)

    Immunohistochemistry analysis of Endostatin showing positive staining in the secretion of paraffin-treated Human colon tissue (right) compared with a negative control in the absence of primary antibody (left). To expose target proteins, antigen retrieval method was performed using 10mM sodium citrate (pH 6.0), microwaved for 8-15 min. Following antigen retrieval, tissues were blocked in 3% H2O2-methanol for 15 min at room temperature, washed with ddH2O and PBS, and then probed with an endostatin polyclonal antibody (Product # PA1-601) diluted by 3% BSA-PBS at a dilution of 1:200 overnight at 4°C in a humidified chamber. Tissues were washed extensively PBST and detection was performed using an HRP-conjugated secondary antibody followed by colorimetric detection using a DAB kit. Tissues were counterstained with hematoxylin and dehydrated with ethanol and xylene to prep for mounting.
  • Endostatin Antibody (PA1-601) in IHC (P)
    PA1-601_IHC_mouse-kidney-tissue.jpg

    Immunohistochemistry (Paraffin) with anti-Endostatin Polyclonal Antibody (PA1-601) (PA1-601_IHC_mouse-kidney-tissue.jpg)

    Immunohistochemistry (Paraffin) with anti-Endostatin Polyclonal Antibody (PA1-601)

    Immunohistochemistry analysis of Endostatin showing positive staining in the secretion of paraffin-treated Mouse kidney tissue (right) compared with a negative control in the absence of primary antibody (left). To expose target proteins, antigen retrieval method was performed using 10mM sodium citrate (pH 6.0), microwaved for 8-15 min. Following antigen retrieval, tissues were blocked in 3% H2O2-methanol for 15 min at room temperature, washed with ddH2O and PBS, and then probed with an endostatin polyclonal antibody (Product # PA1-601) diluted by 3% BSA-PBS at a dilution of 1:100 overnight at 4°C in a humidified chamber. Tissues were washed extensively PBST and detection was performed using an HRP-conjugated secondary antibody followed by colorimetric detection using a DAB kit. Tissues were counterstained with hematoxylin and dehydrated with ethanol and xylene to prep for mounting.
Publications:
Western Blot
  Species / Dilution Summary
  Ms / Not Cited
PA1-601 was used in immunohistochemistry and western blot to study the effect of antiangiogenic gene therapy on tumor metastases

Cancer Gene Ther. 2005 Jan;12(1):35-45.
"Intramuscular delivery of antiangiogenic genes suppresses secondary metastases after removal of primary tumors."
Author(s): Sun X, Qiao H, Jiang H, Zhi X, Liu F, Wang J, Liu M, Dong D, Kanwar JR, Xu R, Krissansen GW
Number of Citations: 1
(See PubMed article )
  Ms / Not Cited
PA1-601 was used in immunohistochemistry and western blot to investigate the effect of endostatin gene therapy on the efficacy of paclitaxel against breast cancer

J Biomed Sci. 2008 Jan;15(1):99-109.
"Endostatin gene therapy enhances the efficacy of paclitaxel to suppress breast cancers and metastases in mice."
Author(s): Li J, Dong X, Xu Z, Jiang X, Jiang H, Krissansen GW, Sun X
Number of Citations: 1
(See PubMed article )
  Nhp / 0
PA1-601 was used in western blot to investigate the effect of gene transfer of endostatin on the therapeutic efficacy of doxorubicin against human hepatocellular carcinomas

Cancer Sci. 2007 Sep;98(9):1381-7.
"Gene transfer of endostatin enhances the efficacy of doxorubicin to suppress human hepatocellular carcinomas in mice."
Author(s): Liu F, Tan G, Li J, Dong X, Krissansen GW, Sun X
Number of Citations: 1
(See PubMed article )
Immunohistochemistry
  Species / Dilution Summary
  Ms / Not Cited
PA1-601 was used in immunohistochemistry and western blot to study the effect of antiangiogenic gene therapy on tumor metastases

Cancer Gene Ther. 2005 Jan;12(1):35-45.
"Intramuscular delivery of antiangiogenic genes suppresses secondary metastases after removal of primary tumors."
Author(s): Sun X, Qiao H, Jiang H, Zhi X, Liu F, Wang J, Liu M, Dong D, Kanwar JR, Xu R, Krissansen GW
Number of Citations: 1
(See PubMed article )
  Ms / Not Cited
PA1-601 was used in immunohistochemistry and western blot to investigate the effect of endostatin gene therapy on the efficacy of paclitaxel against breast cancer

J Biomed Sci. 2008 Jan;15(1):99-109.
"Endostatin gene therapy enhances the efficacy of paclitaxel to suppress breast cancers and metastases in mice."
Author(s): Li J, Dong X, Xu Z, Jiang X, Jiang H, Krissansen GW, Sun X
Number of Citations: 1
(See PubMed article )
(This product is for In Vitro experimental use only. Not for resale without express authorization.)
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