| Details | |
| Host / Isotype: | Rat / IgG2b |
| Class: | Polyclonal |
| Type: | Antibody |
| Clone: | JF4 |
| Species Reactivity: | Human (Hu) Mouse (Ms) |
| Immunogen: | Recombinant fragment of mouse nidogen. |
| Ordering Information | ||||
| Pierce Enactin G2 Domain Antibody (JF4) |
| Storage: | -20° C, Avoid Freeze/Thaw Cycles |
| Form: | 50 µl affinity purified antibody from culture supernatant in 0.5 M acetic acid neutralized with 2 M Trizma Base, plus 0.1% sodium azide. |
| Applications | Dilution * |
| Western Blot (WB) | Assay Dependent |
| ELISA (ELISA) | Assay Dependent |
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* Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own experiment using appropriate negative and positive controls.
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| Product Specific Information |
| Enactin-G2 Domain in mouse and human samples. PA1-5845 has been successfully used in Western blot and ELISA procedures. The PA1-5845 immunogen is recombinant fragment corresponding to the G2 region of mouse nidogen. |
| General Information |
| Basement membrane components play an important role in cell adhesion, locomotion, differentiation and embryonic compartmentalization. Although the precise composition and structure of basal laminae varies from tissue to tissue, as well as within the same tissue at different developmental stages, most mature basal laminae contain type IV collagen,laminins, heparan sulphate proteoglycans and entactin (or nidogen). Entactin is a sulphated glycoprotein of approximately ~150 kD. |