* Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own experiment using appropriate negative and positive controls.
Antigen affinity chromatography
0.1M tris, pH 7,
20% glycerol, 0.1M glycine
-20° C, Avoid Freeze/Thaw Cycles
Product Specific Information
PA5-29905 targets EWSR1 in IF, IHC (P), and WB applications and shows reactivity with Human samples.
The PA5-29905 immunogen is recombinant fragment corresponding to a region within amino acids 1 and 224 of Human EWSR1.
This gene encodes a putative RNA binding protein. Mutations in this gene, specifically a t(11;22)(q24;q12) translocation, are known to cause Ewing sarcoma as well as neuroectodermal and various other tumors. Alternative splicing of this gene results in two products.
EWSR1 Antibody (PA5-29905) in WB PA5-29905_WB.jpg
Western Blot with anti-EWSR1 Polyclonal Antibody (PA5-29905)
Western blot analysis of EWSR1 using Various whole cell extracts (30 ?g). Samples were loaded onto a 10% SDS-PAGE gel and probed with an EWSR1 polyclonal antibody (Product # PA5-29905) at a dilution of 1:1000.
EWSR1 Antibody (PA5-29905) in IF PA5-29905_IF.jpg
Immunofluorescence with anti-EWSR1 Polyclonal Antibody (PA5-29905)