* Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own experiment using appropriate negative and positive controls.
Antigen affinity chromatography
0.09% sodium azide
Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C
Product Specific Information
This antibody is predicted to react with bovine, mouse, rat and Xenopus and zebrafish based on sequence homology.
Component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is required for several steps in the initiation of protein synthesis. The eIF-3 complex associates with the 40S ribosome and facilitates the recruitment of eIF-1, eIF-1A, eIF-2:GTP:methionyl-tRNAi and eIF-5 to form the 43S preinitiation complex (43S PIC). The eIF-3 complex stimulates mRNA recruitment to the 43S PIC and scanning of the mRNA for AUG recognition. The eIF-3 complex is also required for disassembly and recycling of posttermination ribosomal complexes and subsequently prevents premature joining of the 40S and 60S ribosomal subunits prior to initiation. This subunit can bind 18S rRNA.
EIF3G Antibody (PA5-25261) in WB EIF3G_Antibody_PA5-25261_WB_1_112013.jpg
Western Blot with anti-EIF3G Polyclonal Antibody (PA5-25261)
Immunohistochemistry analysis in formalin-fixed, paraffin-embedded human cervix carcinoma using an EIF3G polyclonal antibody (Product # PA5-25261), followed by HRP-conjugated secondary antibody and DAB staining.
EIF3G Antibody (PA5-25261) in FACS EIF3G_Antibody_PA5-25261_FC_1_112013.jpg
Flow Cytometry with anti-EIF3G Polyclonal Antibody (PA5-25261)
Flow cytometry analysis of K562 cells using an EIF3G polyclonal antibody (Product # PA5-25261) (bottom) compared to a negative control cell (top) at a dilution of 1:10-50, followed by a FITC-conjugated goat anti-rabbit antibody
(This product is for In Vitro experimental use only. Not for resale without express authorization.)