EIF3G Antibody

EIF3G Polyclonal Antibody for Western blot, IHC, FACS

>> See 3 other antibodies for EIF3G (EIF3-P42, EIF3S4, eIF3-delta, eIF3-p44, eIF-3 RNA-binding subunit, eIF-3-delta, eIF3 p42, eIF3 p44, eukaryotic translation initiation factor 3 RNA-binding subunit, eukaryotic translation initiation factor 3 subunit 4, eukaryotic translation initiation factor 3 subunit G, eukaryotic translation initiat)
Synonyms:
EIF3-P42, EIF3S4, eIF3-delta, eIF3-p44, eIF-3 RNA-binding subunit, eIF-3-delta, eIF3 p42, eIF3 p44, eukaryotic translation initiation factor 3 RNA-binding subunit, eukaryotic translation initiation factor 3 subunit 4, eukaryotic translation initiation factor 3 subunit G, eukaryotic translation initiat
Entrez Gene ID:
UniProt ID:
Details
Host / Isotype: Rabbit / IgG
Class: Polyclonal
Type: Antibody
Tested Species Reactivity: Human (Hu)
Immunogen: KLH conjugated synthetic peptide between 209-238 amino acids from the central region of human EIF3G
Ordering Information
Pierce EIF3G Antibody
Product #
PA5-25261
Size
400 ul
Price
$316.00
Purchase
Add EIF3G Antibody to your cart.
Tested Applications Dilution *
Western Blot (WB) 1:1000
Immunohistochemistry (IHC) 1:50-1:100
Flow Cytometry (FACS) 1:10-1:50
* Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own experiment using appropriate negative and positive controls.
Form Information
Form: Liquid
Concentration: Lot-specific
Purification: Antigen affinity chromatography
Storage Buffer: PBS
Preservative: 0.09% sodium azide
Storage Conditions: Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C
Product Specific Information
This antibody is predicted to react with bovine, mouse, rat and Xenopus and zebrafish based on sequence homology.
General Information
Component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is required for several steps in the initiation of protein synthesis. The eIF-3 complex associates with the 40S ribosome and facilitates the recruitment of eIF-1, eIF-1A, eIF-2:GTP:methionyl-tRNAi and eIF-5 to form the 43S preinitiation complex (43S PIC). The eIF-3 complex stimulates mRNA recruitment to the 43S PIC and scanning of the mRNA for AUG recognition. The eIF-3 complex is also required for disassembly and recycling of posttermination ribosomal complexes and subsequently prevents premature joining of the 40S and 60S ribosomal subunits prior to initiation. This subunit can bind 18S rRNA.
Product Images
  • EIF3G Antibody (PA5-25261) in WB
    EIF3G_Antibody_PA5-25261_WB_1_112013.jpg

    Western Blot with anti-EIF3G Polyclonal Antibody (PA5-25261) (EIF3G_Antibody_PA5-25261_WB_1_112013.jpg)

    Western Blot with anti-EIF3G Polyclonal Antibody (PA5-25261)

    Western blot analysis using an EIF3G polyclonal antibody (Product # PA5-25261) in K562 cell lysates (35ug per lane).
  • EIF3G Antibody (PA5-25261) in IHC
    EIF3G_Antibody_PA5-25261_IHC_1_112013.jpg

    Immunohistochemistry with anti-EIF3G Polyclonal Antibody (PA5-25261) (EIF3G_Antibody_PA5-25261_IHC_1_112013.jpg)

    Immunohistochemistry with anti-EIF3G Polyclonal Antibody (PA5-25261)

    Immunohistochemistry analysis in formalin-fixed, paraffin-embedded human cervix carcinoma using an EIF3G polyclonal antibody (Product # PA5-25261), followed by HRP-conjugated secondary antibody and DAB staining.
  • EIF3G Antibody (PA5-25261) in FACS
    EIF3G_Antibody_PA5-25261_FC_1_112013.jpg

    Flow Cytometry with anti-EIF3G Polyclonal Antibody (PA5-25261) (EIF3G_Antibody_PA5-25261_FC_1_112013.jpg)

    Flow Cytometry with anti-EIF3G Polyclonal Antibody (PA5-25261)

    Flow cytometry analysis of K562 cells using an EIF3G polyclonal antibody (Product # PA5-25261) (bottom) compared to a negative control cell (top) at a dilution of 1:10-50, followed by a FITC-conjugated goat anti-rabbit antibody
(This product is for In Vitro experimental use only. Not for resale without express authorization.)
Part of Thermo Fisher Scientific