* Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own experiment using appropriate negative and positive controls.
Antigen affinity chromatography
0.09% sodium azide
Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C
Product Specific Information
PA5-26104 targets EFEMP1 in FACS, IHC, and WB applications and shows reactivity with Human and mouse samples.
The PA5-26104 immunogen is synthetic peptide from the N-terminal region of human EFEMP1 conjugated to KLH.
Fibulin 3, also known as epidermal growth factor (EGF)-containing fibulin-like extracellular matrix protein 1 (EFEMP1), is a member of the fibulin family of extracellular glycoproteins, a group of proteins that are widely distributed and frequently associated with vascular and elastic tissues. The fibulin proteins typically contain a tandem array of EGF-like repeats and a fibulin-type COOH-terminal module. Aberrant accumulation of Fibulin 3 in the endoplasmic reticulum of retinal pigment epithelial cells has been shown to be associated with inherited forms of macular degeneration, but the loss of Fibulin 3 expression does not lead to macular degeneration but rather the appearance of hernias due to a reduction of elastic fibers of fascial connective tissue. Recent experiments have shown that expression of Fibulin 3 promotes tumor growth and may thus be a therapeutic target. At least three isoforms of Fibulin 3 are known to exist. This antibody is predicted to not cross-react with other Fibulin proteins.
EFEMP1 Antibody (PA5-26104) in WB EFEMP1_Antibody_PA5-26104_WB_1_112013.jpg
Western Blot with anti-EFEMP1 Polyclonal Antibody (PA5-26104)
Immunohistochemical staining of formalin-fixed and paraffin-embedded human lung carcinoma tissue using anti-EFEMP1 Polyclonal Antibody (PA5-26104), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
EFEMP1 Antibody (PA5-26104) in FACS EFEMP1_Antibody_PA5-26104_FC_1_112013.jpg
Flow Cytometry with anti-EFEMP1 Polyclonal Antibody (PA5-26104)
Flow cytometric analysis of NCI-H460 cells using anti-EFEMP1 Polyclonal Antibody (PA5-26104) (bottom) compared to a negative control cell (top) at a dilution of 1:10-50. Secondary antibody used to visualize was a Goat Anti-Rabbit Secondary Antibody Conjugated to FITC.
(This product is for In Vitro experimental use only. Not for resale without express authorization.)