EDG1 Antibody
EDG1 Polyclonal Antibody for IF, IHC, IP, Western blot
Synonyms:
Sphingolipid Receptor 1, Endothelial Differentiation Gene 1, EDG1
Entrez Gene ID:
UniProt ID:
(Hu) P21453, (Ms) O08530
| Details | |
| Host / Isotype: | Rabbit |
| Class: | Polyclonal |
| Type: | Antibody |
| Species Reactivity: | Human (Hu) Mouse (Ms) |
| Immunogen: | Synthetic peptide corresponding to residues S(359) H P Q K D D G D N P E T I(372) of mouse S1P1. |
| Ordering Information | ||||
| Pierce EDG1 Antibody |
| Storage: | -20° C, Avoid Freeze/Thaw Cycles |
| Form: | 100 µg of epitope affinity purified antibody (1 mg/ml) in PBS containing 1 mg/ml BSA and 0.05% sodium azide. |
| Applications | Dilution * |
| Immunofluorescence (IF) | 5 ug/ml |
| Immunohistochemistry (IHC) | Assay dependent |
| Western Blot (WB) | 1 ug/ml |
| Immunoprecipitation (IP) | Assay dependent |
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* Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own experiment using appropriate negative and positive controls.
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| Product Specific Information |
| PA1-1040 detects S1P1 protein in transfected human and mouse cell samples. This antibody shows no cross-reactivity to S1P3. PA1-1040 has successfully been used in immuno-fluorescence, immunohistochemistry, immunoprecipitation and Western blot procedures. By Western blot, this antibody detects an ~44 kDa protein representing recombinant S1P1 from transfected HEK293 cells. Immunofluorescence data demonstrates that S1P1 is localized to the plasma membrane after staining with PA1-1040. The PA1-1040 immunogen is a synthetic peptide corresponding to residues S(359) H P Q K D D G D N P E T I(372) of mouse S1P1. This sequence is completely conserved in rat and 92% conserved in human S1P1. This peptide (Cat. # PEP-220) is available for use in neutralization and control experiments. |
| General Information |
| Sphingosine 1-phosphate (S1P) is bioactive lipid derived from the metabolism of sphingomyelin. S1P is predominately synthesized, stored, and released from platelets. When released, this molecule stimulates signaling pathways via G-protein coupled receptors. One such receptor is the heterotrimeric guanine nucleotide binding protein shingosine 1-phosphate receptor type 1 (S1P1,also known as EDG1). Research has demonstrated that S1P regulates numerous cellular responses including, wound healing, cell growth, prevention of apoptosis, vascular maturation and cellular migration and survival. In studies involving S1P1 deficient embryos, blood vessels where incompletely maturated with smooth muscles cells, resulting in embryonic death. In contrast, a separate study demonstrated that overexpression of S1P1 led to the stimulation of cell migration. |
| Product Images |
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EDG1 Antibody (PA1-1040) in IF
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Immunofluorescence with anti-EDG1 Polyclonal Antibody (PA1-1040)
Immunofluorescence staining of S1P1 in transfected CCL39 cells using PA1-1040. -
EDG1 Antibody (PA1-1040) in WB
Western Blot with anti-EDG1 Polyclonal Antibody (PA1-1040)
Western blot detection of S1P1 from transfected HEK293 cell lysate using PA1-1040.
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| PubMed References: |
| Immunohistochemistry | ||
| Species / Dilution | Summary | |
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Hu / 1:200 |
PA1-1040 was used in immunohistochemistry and western blot to investigate the role of sphingosine-1-phosphate receptor type 1 in glioma cell proliferation and its prognostic significance
Int J Cancer. 2010 May 15;126(10):2341-52.
"Sphingosine-1-phosphate receptor type 1 regulates glioma cell proliferation and correlates with patient survival." Author(s): Yoshida Y, Nakada M, Sugimoto N, Harada T, Hayashi Y, Kita D, Uchiyama N, Hayashi Y, Yachie A, Takuwa Y, Hamada J Number of Citations: 3 (See PubMed article  )
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Ms / 0 |
PA1-1040 was used in immunohistochemistry to study the regulatory role of stress-induced chemokines on dendritic cell trafficking in skin
Nat Immunol. 2012 Jun 24;13(8):744-52.
"Stress-induced production of chemokines by hair follicles regulates the trafficking of dendritic cells in skin." Author(s): Nagao K, Kobayashi T, Moro K, Ohyama M, Adachi T, Kitashima DY, Ueha S, Horiuchi K, Tanizaki H, Kabashima K, Kubo A, Cho YH, Clausen BE, Matsushima K, Suematsu M, Furtado GC, Lira SA, Farber JM, Udey MC, Amagai M Number of Citations: 0 (See PubMed article )
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| Immunoprecipitation | ||
| Species / Dilution | Summary | |
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Hu / Not Cited |
PA1-1040 was used in immunoprecipitation and western blot to investigate the role of sphingosine kinase 1 S1P in the GAP43 expression stimulated by glial cell line-derived neurotrophic factor
J Cell Biochem. 2011 Nov;112(11):3449-58.
"Sphingosine kinase 1/S1P pathway involvement in the GDNF-induced GAP43 transcription." Author(s): Murakami M, Ito H, Hagiwara K, Kobayashi M, Hoshikawa A, Takagi A, Kojima T, Tamiya-Koizumi K, Sobue S, Ichihara M, Suzuki M, Banno Y, Nozawa Y, Murate T Number of Citations: 0 (See PubMed article )
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| Western Blot | ||
| Species / Dilution | Summary | |
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Hu / 1:1000 |
PA1-1040 was used in immunohistochemistry and western blot to investigate the role of sphingosine-1-phosphate receptor type 1 in glioma cell proliferation and its prognostic significance
Int J Cancer. 2010 May 15;126(10):2341-52.
"Sphingosine-1-phosphate receptor type 1 regulates glioma cell proliferation and correlates with patient survival." Author(s): Yoshida Y, Nakada M, Sugimoto N, Harada T, Hayashi Y, Kita D, Uchiyama N, Hayashi Y, Yachie A, Takuwa Y, Hamada J Number of Citations: 3 (See PubMed article )
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Hu / Not Cited |
PA1-1040 was used in western blot to investigate the effect of the polysaccharide MDG-1 on specific gene expression and cell survival in ischemic hearts
Glycobiology. 2010 Jan;20(4):473-84.
"A polysaccharide, MDG-1, induces S1P1 and bFGF expression and augments survival and angiogenesis in the ischemic heart." Author(s): Wang S, Zhang Z, Lin X, Xu DS, Feng Y, Ding K Number of Citations: 3 (See PubMed article )
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Hu / Not Cited |
PA1-1040 was used in immunoprecipitation and western blot to investigate the role of sphingosine kinase 1 S1P in the GAP43 expression stimulated by glial cell line-derived neurotrophic factor
J Cell Biochem. 2011 Nov;112(11):3449-58.
"Sphingosine kinase 1/S1P pathway involvement in the GDNF-induced GAP43 transcription." Author(s): Murakami M, Ito H, Hagiwara K, Kobayashi M, Hoshikawa A, Takagi A, Kojima T, Tamiya-Koizumi K, Sobue S, Ichihara M, Suzuki M, Banno Y, Nozawa Y, Murate T Number of Citations: 0 (See PubMed article )
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(This product is for In Vitro experimental use only. Not for resale without express authorization.)
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EDG1 Antibodies > EDG1 Polyclonal Antibody PA1-1040
