E2F6 Antibody

(This product has been discontinued.)
Please click the link below to find suitable replacements for this discontinued product.

>> See 2 other antibodies for E2F6

E2F6 Polyclonal Antibody for Western blot

>> See 2 other antibodies for E2F6 (Transcription factor E2F6; E2F-6; E2F6)
Synonyms:
Transcription factor E2F6; E2F-6; E2F6
Entrez Gene ID:
UniProt ID:
Details
Host / Isotype: Rabbit
Class: Polyclonal
Type: Antibody
Tested Species Reactivity: Human (Hu)
Immunogen: Synthetic peptide from the center region of E2F6 conjugated to KLH.
Ordering Information
Pierce E2F6 Antibody
Product #
PA5-13589
Size
400 ul
Price
$316.00
Purchase
(DISCONTINUED)
Tested Applications Dilution *
Western Blot (WB) 1:50-1:100
* Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own experiment using appropriate negative and positive controls.
Form Information
Form: Liquid
Concentration: Lot-specific
Purification: Ammonium sulfate precipitation, Size-exclusion - Dialysis
Storage Buffer: PBS
Preservative: 0.09% sodium azide
Storage Conditions: Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C
Product Specific Information
PA5-13589 targets E2F6 in WB applications and shows reactivity with Human samples. The PA5-13589 immunogen is a synthetic peptide from the center region of E2F6 conjugated to KLH. PA5-13589 detects E2F6 which has a predicted molecular weight of approximately 32 kDa.
General Information
This gene encodes a member of the E2F transcription factor protein family. E2F family members play a crucial role in control of the cell cycle and of the action of tumor suppressor proteins. They are also a target of the transforming proteins of small DNA tumor viruses. Many E2F proteins contain several evolutionarily conserved domains: a DNA binding domain, a dimerization domain which determines interaction with the differentiation regulated transcription factor proteins, a transactivation domain enriched in acidic amino acids, and a tumor suppressor protein association domain which is embedded within the transactivation domain. The encoded protein of this gene is atypical because it lacks the transactivation and tumor suppressor protein association domains. It contains a modular suppression domain and is an inhibitor of E2F-dependent transcription. The protein is part of a multimeric protein complex that contains a histone methyltransferase and the transcription factors Mga and Max. Multiple transcript variants have been reported for this gene, but it has not been clearly demonstrated that they encode valid isoforms.
Product Images
  • E2F6 Antibody (PA5-13589) in WB
    E2F6_Antibody_PA5-13589_WB_1_112013.jpg

    Western Blot with anti-E2F6 Polyclonal Antibody (PA5-13589) (E2F6_Antibody_PA5-13589_WB_1_112013.jpg)

    Western Blot with anti-E2F6 Polyclonal Antibody (PA5-13589)

    Western blot analysis of anti-E2F6 Polyclonal Antibody (PA5-13589) in HL60 cell line lysates (35 ug per lane).
  • E2F6 Antibody (PA5-13589) in FACS
    E2F6_Antibody_PA5-13589_FC_1_112013.jpg

    Flow Cytometry with anti-E2F6 Polyclonal Antibody (PA5-13589) (E2F6_Antibody_PA5-13589_FC_1_112013.jpg)

    Flow Cytometry with anti-E2F6 Polyclonal Antibody (PA5-13589)

    Flow cytometric analysis of NCI-H292 cells using anti-E2F6 Polyclonal Antibody (PA5-13589) (bottom), compared to a negative control cell (top) at a dilution of 1:10-50. Secondary antibody used to visualize was a Goat Anti-Rabbit Secondary Antibody Conjugated to FITC.
(This product is for In Vitro experimental use only. Not for resale without express authorization.)
Part of Thermo Fisher Scientific