Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C
100 µg antibody (0.25 mg/ml) in PBS with 0.09% (W/V) sodium azide.
Flow Cytometry (FACS)
1:10 - 1:50
Western Blot (WB)
* Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own experiment using appropriate negative and positive controls.
Product Specific Information
PA5-11548 targets E Cadherin in Western blot and flow cytometry applications and shows reactivity with Human and Mouse samples.
The PA5-11548 immunogen is a synthetic peptide from the N-terminal region of CDH1 conjugated to KLH.
PA5-11548 detects E Cadherin which has a predicted molecular weight of approximately 97 kDa.
This gene is a classical cadherin from the cadherin superfamily. The encoded protein is a calcium dependent cell-cell adhesion glycoprotein comprised of five extracellular cadherin repeats, a transmembrane region and a highly conserved cytoplasmic tail. Mutations in this gene are correlated with gastric, breast, colorectal, thyroid and ovarian cancer. Loss of function is thought to contribute to progression in cancer by increasing proliferation, invasion, and/or metastasis. The ectodomain of this protein mediates bacterial adhesion to mammalian cells and the cytoplasmic domain is required for internalization. Identified transcript variants arise from mutation at consensus splice sites.
E Cadherin Antibody (PA5-11548) in WB
Western Blot with anti-E Cadherin Polyclonal Antibody (PA5-11548)