Desmin Antibody (D33)

Desmin Monoclonal Antibody for IF, ICC, IHC (P)

Synonyms:
intermediate filament protein
Details
Host / Isotype: Mouse / IgG1, kappa
Class: Monoclonal
Type: Antibody
Clone: D33
Tested Species Reactivity: Human (Hu), Rat (Rt), Bovine (Bv), Canine (Ca), Chicken (Ck), Feline (Fe), Hamster (Hm), Non-human primate (Nhp)
Published Species Reactivity: Canine (Ca), Human (Hu), Rat (Rt)
Immunogen: Desmin from human muscle
Ordering Information
Pierce Desmin Antibody (D33)
Product #
MA5-13259
Size
500 ul
Price
$265.00
Purchase
Add Desmin Antibody (D33) to your cart.
Tested Applications Dilution *
Immunofluorescence (IF) 1:10-1:100
Immunocytochemistry (ICC) 1:10-1:100
Immunohistochemistry (Paraffin) (IHC (P)) 1:50-1:100
Published Applications Dilution
Immunocytochemistry (ICC) See publications below
Immunohistochemistry (IHC) See publications below
Flow Cytometry (FACS) See publications below
* Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own experiment using appropriate negative and positive controls.
Form Information
Form: Liquid
Storage Buffer: tissue culture supernatant
Preservative: 0.09% sodium azide
Storage Conditions: 4° C
Product Specific Information
MA5-13259 targets Desmin in IF and IHC (P) applications and shows reactivity with Bovine, Canine, Chicken, Feline, Hamster, Human, Non-human primate, and Rat samples.

The MA5-13259 immunogen is desmin from human muscle.
General Information
Desmin is an intermediate filament protein of both smooth and striated muscles. Antibody to desmin reacts with striated (skeletal and cardiac) as well as smooth muscle cells. In skeletal and cardiac muscles, the staining is confined to the Z-bands giving a characteristic striated appearance. Anti-desmin antibody is useful in identification of tumors of myogenic origin. It reacts with leiomyosarcomas (smooth muscle) as well as rhabdomyosarcomas (striated muscle).
Product Images
  • Desmin Antibody (MA5-13259) in IF
    MA5-13259_IF_human_skeletal_muscle_20131018123837.jpg

    Immunofluorescence with anti-Desmin Monoclonal Antibody [D33] (MA5-13259) (MA5-13259_IF_human_skeletal_muscle_20131018123837.jpg)

    Immunofluorescence with anti-Desmin Monoclonal Antibody [D33] (MA5-13259)

    Immunofluorescent analysis of Desmin (green) showing staining in the cytoplasm of human skeletal muscle (right) compared to a negative control without primary antibody (left). Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with a Desmin monoclonal antibody (Product # MA5-13259) in 3% BSA-PBS at a dilution of 1:20 and incubated overnight at 4ºC in a humidified chamber. Cells were washed with PBST and incubated with a DyLight-conjugated secondary antibody in PBS at room temperature in the dark. F-actin (red) was stained with a fluorescent red phalloidin and nuclei (blue) were stained with Hoechst or DAPI. Images were taken at a magnification of 60x.
  • Desmin Antibody (MA5-13259) in IHC
    MA5-13259_Immunohistochemistry.jpg

    Immunohistochemistry with anti-Desmin Monoclonal Antibody [D33] (MA5-13259) (MA5-13259_Immunohistochemistry.jpg)

    Immunohistochemistry with anti-Desmin Monoclonal Antibody [D33] (MA5-13259)

    Formalin-fixed, paraffin-embedded human leiomyoma stained with Desmin antibody using peroxidase-conjugate and AEC chromogen. Note cytoplasmic staining of muscle cells.
  • Desmin Antibody (MA5-13259) in IHC
    MA5-13259_Immunohistochemistry2.jpg

    Immunohistochemistry with anti-Desmin Monoclonal Antibody [D33] (MA5-13259) (MA5-13259_Immunohistochemistry2.jpg)

    Immunohistochemistry with anti-Desmin Monoclonal Antibody [D33] (MA5-13259)

    Formalin-fixed, paraffin-embedded rat bladder stained with Desmin antibody using peroxidase-conjugate and AEC chromogen. Note cytoplasmic staining of muscle cells.
Publications:
Immunocytochemistry
  Species / Dilution Summary
  Hu / 1:100
MA5-13259 was used in immunocytochemistry to establish and characterize immortal cell lines from dystrophic muscles

Skelet Muscle. 2011 Mar 8;1(1):12.
"Establishment of clonal myogenic cell lines from severely affected dystrophic muscles - CDK4 maintains the myogenic population."
Author(s): Stadler G, Chen JC, Wagner K, Robin JD, Shay JW, Emerson CP Jr, Wright WE
Number of Citations: 1
(See PubMed article )
Immunohistochemistry
  Species / Dilution Summary
  Ca / 0
MA5-13259 was used in immunohistochemistry to characterize a maxillary myofibroblastic sarcoma in a Dog

J Comp Pathol. 2012 Jul;147(1):42-5.
"Low-grade Myofibroblastic Sarcoma of the Maxillary Region in a Dog."
Author(s): Tsuchiya T, Suzuki K, Hojo Y, Shiraki A, Imaoka M, Shibutani M, Mitsumori K
Number of Citations: 1
(See PubMed article )
  Hu / 1:50
MA5-13259 was used in immunohistochemistry to study the existence of novel routes of tumour spread via open channels of pseudoangiomatous stromal hyperplasia

Histopathology. 2002 Sep;41(3):208-15.
"Malignant neoplasms infiltrating pseudoangiomatous' stromal hyperplasia of the breast: an unrecognized pathway of tumour spread."
Author(s): Damiani S, Eusebi V, Peterse JL
Number of Citations: 2
(See PubMed article )
  Hu / 1:50
MA5-13259 was used in immunohistochemistry to study the cellular origins of myoid cells in benign mammary stromo-epithelial lesions

Histopathology. 2003 May;42(5):448-56.
"Smooth muscle cell differentiation in mammary stromo-epithelial lesions with evidence of a dual origin: stromal myofibroblasts and myoepithelial cells."
Author(s): Di Tommaso L, Pasquinelli G, Damiani S
Number of Citations: 5
(See PubMed article )
  Hu / 0
MA5-13259 was used in immunohistochemistry to report on a patient with collagenous fibroma

Yonsei Med J. 2004 Oct 31;45(5):941-3.
"Collagenous fibroma (desmoplastic fibroblastoma)."
Author(s): Dagli M, Eryilmaz A, Acar A, Kulacoglu S, Akmansu H
Number of Citations: 3
(See PubMed article )
  Hu / 1:150
MA5-13259 was used in immunohistochemistry to evaluate the prognostic significance of multiple factors in gastrointestinal stromal tumors with malignant potential

J Gastrointest Surg. 2005 Mar;9(3):418-29.
"Analysis of prognostic and immunohistochemical factors in gastrointestinal stromal tumors with malignant potential."
Author(s): Ozgüç H, Yilmazlar T, Yerci O, Soylu R, Tümay V, Filiz G, Zorluoglu A
Number of Citations: 3
(See PubMed article )
  Hu / 0
MA5-13259 was used in immunohistochemistry to report on a case of soft tissue sacrococcygeal chordoma with intracytoplasmic filamentous inclusions

Pathol Res Pract. 2005;201(10):699-704.
"Soft tissue sacrococcygeal chordoma with intracytoplasmic filamentous inclusions."
Author(s): Guarino M, Ballabio G, Rubino B, Nebuloni M, Tosoni A
Number of Citations: 0
(See PubMed article )
  Hu / 0
MA5-13259 was used in immunohistochemistry to study two adult patients with mesenchymal hamartoma of the liver

J Hepatobiliary Pancreat Surg. 2005;12(6):502-7.
"Mesenchymal hamartoma of the liver in adulthood: immunohistochemical profiles, clinical and histopathological features in two patients."
Author(s): Yesim G, Gupse T, Zafer U, Ahmet A
Number of Citations: 2
(See PubMed article )
  Hu / Not Cited
MA5-13259 was used in immunohistochemistry to investigate the tumorigenesis of rhabdomyosarcoma through Kit expression and its therapeutic implication

Pathol Res Pract. 2006;202(9):671-7.
"Kit expression in spindle cell rhabdomyosarcoma can possibly create a different approach for its tumorigenesis and therapy."
Author(s): Diniz G, Aktas S, Ortac R, Tunakan M, Unlu I, Vergin C
Number of Citations: 0
(See PubMed article )
  Hu / 0
MA5-13259 was used in immunohistochemistry to report on a case of myointimoma of the glans penis

Pathol Int. 2007 Mar;57(3):158-61.
"Myointimoma of the glans penis."
Author(s): Vardar E, Gunlusoy B, Arslan M, Kececi S
Number of Citations: 0
(See PubMed article )
  Hu / 1:50
MA5-13259 was used in immunohistochemistry to report on a case of epithelioid leiomyosarcoma with rhabdoid features

Wien Klin Wochenschr. 2007;119(17-18):557-60.
"Epithelioid leiomyosarcoma with rhabdoid features."
Author(s): Yorulmaz G, Erdogan G, Pestereli HE, Savas B, Karaveli FS
Number of Citations: 3
(See PubMed article )
  Hu / 0
MA5-13259 was used in immunohistochemistry to report a clinical case of dedifferentiated liposarcoma of the retroperitoneum with unique features

Sarcoma. 2008;2008():658090.
"Dedifferentiated Liposarcoma of the Retroperitoneum with Extensive Leiomyosarcomatous Differentiation and beta-Human Chorionic Gonadotropin Production."
Author(s): Russell MJ, Flynt FL, Harroff AL, Fadare O
Number of Citations: 1
(See PubMed article )
  Hu / 0
MA5-13259 was used in immunohistochemistry to study the incidence of multiple primary sporadic gastrointestinal stromal tumors

Clin Cancer Res. 2008 Sep 15;14(18):5715-21.
"Multiple primary sporadic gastrointestinal stromal tumors in the adult: an underestimated entity."
Author(s): Gasparotto D, Rossi S, Bearzi I, Doglioni C, Marzotto A, Hornick JL, Grizzo A, Sartor C, Mandolesi A, Sciot R, Debiec-Rychter M, Dei Tos AP, Maestro R
Number of Citations: 1
(See PubMed article )
  Hu / 1:50
MA5-13259 was used in immunohistochemistry to report on two cases of dendritic cell sarcomas/tumours of the breast

Virchows Arch. 2009 Mar;454(3):333-9.
"Dendritic cell sarcomas/tumours of the breast: report of two cases."
Author(s): Kapucuoglu N, Percinel S, Ventura T, Lang R, Al-Daraji W, Eusebi V
Number of Citations: 1
(See PubMed article )
  Hu / 0
MA5-13259 was used in immunohistochemistry to study the cyclooxygenase-2 expression and its effect on mesenchymal tumor growth and recurrence

APMIS. 2009 Nov;117(11):825-30.
"Cyclooxygenase-2 expression and connection with tumor recurrence and histopathologic parameters in gastrointestinal stromal tumors."
Author(s): Türköz HK, Alkan I, Si?man S, Ozcan D
Number of Citations: 0
(See PubMed article )
  Hu / 1:100
MA5-13259 was used in immunohistochemistry to report on two cases of giant cell tumor-like lesion of the urinary bladder

Diagn Pathol. 2009 Dec 31;4():48.
"Giant cell tumor-like lesion of the urinary bladder: a report of two cases and literature review; giant cell tumor or undifferentiated carcinoma?"
Author(s): Behzato?lu K, Durak H, Canberk S, Aydin O, Huq GE, Oznur M, Ozyalvaçli G, Yildiz P
Number of Citations: 3
(See PubMed article )
  Hu / 0
MA5-13259 was used in immunohistochemistry to report on a case of ectomesenchymal chondromyxoid tumour of the tongue

Anticancer Res. 2010 Nov;30(11):4685-9.
"Ectomesenchymal chondromyxoid tumour of the tongue. A review of histological and immunohistochemical features."
Author(s): Angiero F
Number of Citations: 1
(See PubMed article )
  Hu / 1:200
MA5-13259 was used in immunohistochemistry to report a clinical case of microscopic lymphangioleiomyomatosis perivascular epithelioid cell neoplasm in uterus

Int J Gynecol Pathol. 2011 Jan;30(1):71-5.
"Microscopic uterine lymphangioleiomyomatosis perivascular epithelioid cell neoplasm: a case report with the earliest manifestation of this enigmatic neoplasm."
Author(s): Clay MR, Gibson P, Lowell J, Cooper K
Number of Citations: 1
(See PubMed article )
  Rt / 0
MA5-13259 was used in immunohistochemistry to study the role of allogenic mesenchymal stem cell transplantation in improved healing of ischemic colon anastomosis

Langenbecks Arch Surg. 2011 Jan;396(1):115-26.
"Mesenchymal stem cells improve the healing of ischemic colonic anastomoses (experimental study)."
Author(s): Adas G, Arikan S, Karatepe O, Kemik O, Ayhan S, Karaoz E, Kamali G, Eryasar B, Ustek D
Number of Citations: 1
(See PubMed article )
Flow Cytometry
  Species / Dilution Summary
  Hu / 0
MA5-13259 was used in flow cytometry to develop tissue-engineered pulmonary artery conduits based on human umbilical cord cells

Ann Thorac Surg. 2002 Jul;74(1):46-52; discussion 52.
"Living, autologous pulmonary artery conduits tissue engineered from human umbilical cord cells."
Author(s): Hoerstrup SP, Kadner A, Breymann C, Maurus CF, Guenter CI, Sodian R, Visjager JF, Zund G, Turina MI
Number of Citations: 8
(See PubMed article )
  Hu / 0
MA5-13259 was used in flow cytometry to study the application of umbilical cord cells in cardiovascular tissue engineering

Ann Thorac Surg. 2002 Oct;74(4):S1422-8.
"Human umbilical cord cells: a new cell source for cardiovascular tissue engineering."
Author(s): Kadner A, Hoerstrup SP, Tracy J, Breymann C, Maurus CF, Melnitchouk S, Kadner G, Zund G, Turina M
Number of Citations: 3
(See PubMed article )
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