DNMT1 / DNA Methyltransferase 1 Antibody

DNMT1 / DNA Methyltransferase 1 Polyclonal Antibody for Western blot

>> See 8 other antibodies for DNMT1 (Dnmt1)
Synonyms:
Dnmt1
Entrez Gene ID:
UniProt ID:
Details
Host / Isotype: Rabbit
Class: Polyclonal
Type: Antibody
Tested Species Reactivity: Human (Hu)
Published Species Reactivity: Mouse (Ms)
Immunogen: Synthetic peptide corresponding to residues A(171) K G P A K R K P Q E E S E(185) of human Dnmt1.
Ordering Information
Pierce DNMT1 / DNA Methyltransferase 1 Antibody
Product #
PA1-880
Size
100 µg
Price
$316.00
Purchase
Add DNMT1 / DNA Methyltransferase 1 Antibody to your cart.
Tested Applications Dilution *
Western Blot (WB) 0.5 - 1.0 µg/ml
Published Applications Dilution
Western Blot (WB) See publications below
* Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own experiment using appropriate negative and positive controls.
Form Information
Form: Liquid
Concentration: 1mg/ml
Purification: Antigen affinity chromatography
Storage Buffer: PBS with 1mg/ml BSA
Preservative: 0.05% sodium azide
Storage Conditions: -20° C, Avoid Freeze/Thaw Cycles
Product Specific Information
PA1-880 detects DNA methyltransferase 1 (Dmnt1) from HeLa cell nuclear extracts.

PA1-880 has been successfully used in Western blot procedures. By Western blot, this antibody detects an ~180 kDa protein representing Dnmt1 from HeLa cell nuclear extract. A doublet between 40 and 45 kDa is also seen on Western blots using this antibody.

The PA1-880 immunogen is a synthetic peptide corresponding to residues A(171) K G P A K R K P Q E E S E(185) of human Dnmt1. PA1-880 immunizing peptide (Cat. # PEP-136) is available for use in neutralization and control experiments.
General Information
DNA methyltransferases (Dnmts) methylate the 5-position of cytosine in the context of CpG dinucleotides. DNA methylation is crucial for normal embryonic development, genetic imprinting, and X-chromosome inactivation. Hypermethylation of DNA has been implicated in tumorigenesis through improper methylation of growth regulatory gene promoter regions. There exist three isoforms of Dnmts, Dnmt1, 2 and 3. Dnmt1 is believed to perform most of the maintenance and de novo methylation activities that occur in somatic cells of mammals. Studies have shown that Dnmt1 can establish repression of transcription complexes consisting of Dnmt1, histone deacetylase 2 (HDAC2), and DNA methyltransferase 1 associated protein (DMAP1). Therefore, in addition to DNA methylation maintenance, Dnmt1 is thought to directly target transcriptionally repressive chromatin to the genome during S-phase DNA replication.
Product Images
  • DNMT1 / DNA Methyltransferase 1 Antibody (PA1-880) in WB
    PA1-880_1_07232010.jpg

    Western Blot with anti-DNMT1  /  DNA Methyltransferase 1 Polyclonal Antibody (PA1-880) (PA1-880_1_07232010.jpg)

    Western Blot with anti-DNMT1 / DNA Methyltransferase 1 Polyclonal Antibody (PA1-880)

    Western blot of Dnmt1 on HeLa cell nuclear extract using PA1-880.
Publications:
Western Blot
  Species / Dilution Summary
  Ms / 1:500
PA1-880 was used in western blot to investigate a DNA methyltransferase 1 isoform's expression level during the differentiation of muscle

Cell Growth Differ. 2000 Oct;11(10):551-9.
"Expression of an alternative Dnmt1 isoform during muscle differentiation."
Author(s): Aguirre-Arteta AM, Grunewald I, Cardoso MC, Leonhardt H
Number of Citations: 4
(See PubMed article )
(This product is for In Vitro experimental use only. Not for resale without express authorization.)
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