* Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own experiment using appropriate negative and positive controls.
Antigen affinity chromatography
0.09% sodium azide
Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C
Product Specific Information
DLAT encodes component E2 of the multi-enzyme pyruvate dehydrogenase complex (PDC). PDC resides in the inner mitochondrial membrane and catalyzes the conversion of pyruvate to acetyl coenzyme A. The protein product of this gene, dihydrolipoamide acetyltransferase, accepts acetyl groups formed by the oxidative decarboxylation of pyruvate and transfers them to coenzyme A. Dihydrolipoamide acetyltransferase is the antigen for antimitochondrial antibodies. These autoantibodies are present in nearly 95% of patients with the autoimmune liver disease primary biliary cirrhosis (PBC). In PBC, activated T lymphocytes attack and destroy epithelial cells in the bile duct where this protein is abnormally distributed and overexpressed. PBC enventually leads to cirrhosis and liver failure.
DLAT Antibody (PA5-25260) in WB DLAT-_Antibody_PA5-25260_WB_1_112013.jpg
Western Blot with anti-DLAT Polyclonal Antibody (PA5-25260)
Immunohistochemistry analysis in formalin-fixed, paraffin-embedded human hepatocarcinoma using a DLAT polyclonal antibody (Product # PA5-25260), followed by HRP-conjugated secondary antibody and DAB staining.
DLAT Antibody (PA5-25260) in FACS DLAT-_Antibody_PA5-25260_FC_1_112013.jpg
Flow Cytometry with anti-DLAT Polyclonal Antibody (PA5-25260)
Flow cytometry analysis of HepG2 cells using a DLAT polyclonal antibody (Product # PA5-25260) (right) compared to a negative control cell (left) at a dilution of 1:10-50, followed by a FITC-conjugated goat anti-rabbit antibody
(This product is for In Vitro experimental use only. Not for resale without express authorization.)