DLAT Antibody

DLAT Polyclonal Antibody for Western blot, IHC, FACS

>> See 1 other antibody for DLAT (DLTA, PDC-E2, PDCE2, 70 kDa mitochondrial autoantigen of primary biliary cirrhosis, E2 component of pyruvate dehydrogenase complex, M2 antigen complex 70 kDa subunit, PBC, dihydrolipoamide acetyltransferase component of pyruvate dehydrogenase complex, dihydrolipoyllysine-residue a)
Synonyms:
DLTA, PDC-E2, PDCE2, 70 kDa mitochondrial autoantigen of primary biliary cirrhosis, E2 component of pyruvate dehydrogenase complex, M2 antigen complex 70 kDa subunit, PBC, dihydrolipoamide acetyltransferase component of pyruvate dehydrogenase complex, dihydrolipoyllysine-residue a
Entrez Gene ID:
UniProt ID:
Details
Host / Isotype: Rabbit / IgG
Class: Polyclonal
Type: Antibody
Tested Species Reactivity: Human (Hu)
Immunogen: KLH conjugated synthetic peptide between 579-607 amino acids from the C-terminal region of human DLAT
Ordering Information
Pierce DLAT Antibody
Product #
PA5-25260
Size
400 ul
Price
$316.00
Purchase
Add DLAT Antibody to your cart.
Tested Applications Dilution *
Western Blot (WB) 1:1000
Immunohistochemistry (IHC) 1:10-1:50
Flow Cytometry (FACS) 1:10-1:50
* Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own experiment using appropriate negative and positive controls.
Form Information
Form: Liquid
Concentration: Lot-specific
Purification: Antigen affinity chromatography
Storage Buffer: PBS
Preservative: 0.09% sodium azide
Storage Conditions: Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C
Product Specific Information
General Information
DLAT encodes component E2 of the multi-enzyme pyruvate dehydrogenase complex (PDC). PDC resides in the inner mitochondrial membrane and catalyzes the conversion of pyruvate to acetyl coenzyme A. The protein product of this gene, dihydrolipoamide acetyltransferase, accepts acetyl groups formed by the oxidative decarboxylation of pyruvate and transfers them to coenzyme A. Dihydrolipoamide acetyltransferase is the antigen for antimitochondrial antibodies. These autoantibodies are present in nearly 95% of patients with the autoimmune liver disease primary biliary cirrhosis (PBC). In PBC, activated T lymphocytes attack and destroy epithelial cells in the bile duct where this protein is abnormally distributed and overexpressed. PBC enventually leads to cirrhosis and liver failure.
Product Images
  • DLAT Antibody (PA5-25260) in WB
    DLAT-_Antibody_PA5-25260_WB_1_112013.jpg

    Western Blot with anti-DLAT Polyclonal Antibody (PA5-25260) (DLAT-_Antibody_PA5-25260_WB_1_112013.jpg)

    Western Blot with anti-DLAT Polyclonal Antibody (PA5-25260)

    Western blot analysis using a DLAT polyclonal antibody (Product # PA5-25260) in K562 (lane 1), HepG2 (lane 2), Jurkat (lane 3) cell lysates (35ug per lane).
  • DLAT Antibody (PA5-25260) in IHC
    DLAT-_Antibody_PA5-25260_IHC_1_112013.jpg

    Immunohistochemistry with anti-DLAT Polyclonal Antibody (PA5-25260) (DLAT-_Antibody_PA5-25260_IHC_1_112013.jpg)

    Immunohistochemistry with anti-DLAT Polyclonal Antibody (PA5-25260)

    Immunohistochemistry analysis in formalin-fixed, paraffin-embedded human hepatocarcinoma using a DLAT polyclonal antibody (Product # PA5-25260), followed by HRP-conjugated secondary antibody and DAB staining.
  • DLAT Antibody (PA5-25260) in FACS
    DLAT-_Antibody_PA5-25260_FC_1_112013.jpg

    Flow Cytometry with anti-DLAT Polyclonal Antibody (PA5-25260) (DLAT-_Antibody_PA5-25260_FC_1_112013.jpg)

    Flow Cytometry with anti-DLAT Polyclonal Antibody (PA5-25260)

    Flow cytometry analysis of HepG2 cells using a DLAT polyclonal antibody (Product # PA5-25260) (right) compared to a negative control cell (left) at a dilution of 1:10-50, followed by a FITC-conjugated goat anti-rabbit antibody
(This product is for In Vitro experimental use only. Not for resale without express authorization.)
Part of Thermo Fisher Scientific