* Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own experiment using appropriate negative and positive controls.
Antigen affinity chromatography
0.09% sodium azide
Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C
Product Specific Information
PA5-25260 targets DLAT in FACS, IHC, and WB applications and shows reactivity with Human samples.
The PA5-25260 immunogen is synthetic peptide from the C-terminal region of human DLAT conjugated to KLH.
The pyruvate dehydrogenase complex catalyzes the overall conversion of pyruvate to acetyl-CoA and CO(2). It contains multiple copies of three enzymatic components: pyruvate dehydrogenase (E1). dihydrolipoamide acetyltransferase (E2) and lipoamide dehydrog
DLAT Antibody (PA5-25260) in WB DLAT-_Antibody_PA5-25260_WB_1_112013.jpg
Western Blot with anti-DLAT Polyclonal Antibody (PA5-25260)
Immunohistochemistry analysis in formalin fixed and paraffin embedded human hepatocarcinoma using anti-DLAT Polyclonal Antibody (PA5-25260), followed by peroxidase conjugation of the secondary antibody and DAB staining.
DLAT Antibody (PA5-25260) in FACS DLAT-_Antibody_PA5-25260_FC_1_112013.jpg
Flow Cytometry with anti-DLAT Polyclonal Antibody (PA5-25260)
Flow cytometric analysis of HepG2 cells using anti-DLAT Polyclonal Antibody (PA5-25260) (right) compared to a negative control cell (left) at a dilution of 1:10-50. Secondary antibody used to visualize was a Goat Anti-Rabbit Secondary Antibody Conjugated to FITC.
(This product is for In Vitro experimental use only. Not for resale without express authorization.)