CBF5, DKC, DKCX, NAP57, NOLA4, XAP101, CBF5 homolog, H/ACA ribonucleoprotein complex subunit 4, cbf5p homolog, nopp140-associated protein of 57 kDa, nucleolar protein NAP57, nucleolar protein family A member 4, snoRNP protein DKC1
* Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own experiment using appropriate negative and positive controls.
Antigen affinity chromatography
0.09% sodium azide
Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C
Product Specific Information
DKC1 is a member of the H/ACA snoRNPs (small nucleolar ribonucleoproteins) gene family. snoRNPs are involved in various aspects of rRNA processing and modification and have been classified into two families: C/D and H/ACA. The H/ACA snoRNPs also include the NOLA1, 2 and 3 proteins. The protein encoded by this gene and the three NOLA proteins localize to the dense fibrillar components of nucleoli and to coiled (Cajal) bodies in the nucleus. Both 18S rRNA production and rRNA pseudouridylation are impaired if any one of the four proteins is depleted. These four H/ACA snoRNP proteins are also components of the telomerase complex. The protein encoded by this gene is related to the Saccharomyces cerevisiae Cbf5p and Drosophila melanogaster Nop60B proteins. The gene lies in a tail-to-tail orientation with the palmitoylated erythrocyte membrane protein gene and is transcribed in a telomere to centromere direction. Both nucleotide substitutions and single trinucleotide repeat polymorphisms have been found in this gene. Mutations in this gene cause X-linked dyskeratosis congenita, a disease resulting in reticulate skin pigmentation, mucosal leukoplakia, nail dystrophy, and progressive bone marrow failure in most cases.
DKC1 Antibody (PA5-25393) in WB DKC1_Antibody_PA5-25393_WB_1_112013.jpg
Western Blot with anti-DKC1 Polyclonal Antibody (PA5-25393)
Immunofluorescent analysis of 293 cells using a DKC1 polyclonal antibody (Product # PA5-25393) at a dilution of 1:10-50, followed by a fluor-conjugated goat anti-rabbit secondary antibody (green). Nuclei were stained with DAPI (blue).
DKC1 Antibody (PA5-25393) in IHC DKC1_Antibody_PA5-25393_IHC_1_112013.jpg
Immunohistochemistry with anti-DKC1 Polyclonal Antibody (PA5-25393)
Immunohistochemistry analysis in formalin-fixed, paraffin-embedded human prostate carcinoma using a DKC1 polyclonal antibody (Product # PA5-25393), followed by HRP-conjugated secondary antibody and DAB staining.
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