CBF5, DKC, DKCX, NAP57, NOLA4, XAP101, CBF5 homolog, H/ACA ribonucleoprotein complex subunit 4, cbf5p homolog, nopp140-associated protein of 57 kDa, nucleolar protein NAP57, nucleolar protein family A member 4, snoRNP protein DKC1
* Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own experiment using appropriate negative and positive controls.
Antigen affinity chromatography
0.09% sodium azide
Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C
Product Specific Information
PA5-25393 targets DKC1 in IF, IHC, and WB applications and shows reactivity with Human and mouse samples.
The PA5-25393 immunogen is synthetic peptide from the Central region of human DKC1 conjugated to KLH.
This gene is a member of the H/ACA snoRNPs gene family. snoRNPs are involved in various aspects of rRNA processing and modification and have been classified into two families: C/D and H/ACA. The H/ACA snoRNPs also include the NOLA1, 2 and 3 proteins. The protein encoded by this gene and the three NOLA proteins localize to the dense fibrillar components of nucleoli and to coiled bodies in the nucleus. Both 18S rRNA production and rRNA pseudouridylation are impaired if any one of the four proteins is depleted. These four H/ACA snoRNP proteins are also components of the telomerase complex. The protein encoded by this gene is related to the Saccharomyces cerevisiae Cbf5p and Drosophila melanogaster Nop60B proteins. The gene lies in a tail-to-tail orientation with the palmitoylated erythrocyte membrane protein gene and is transcribed in a telomere to centromere direction. Both nucleotide substitutions and single trinucleotide repeat polymorphisms have been found in this gene. Mutations in this gene cause X-linked dyskeratosis congenita, a disease resulting in reticulate skin pigmentation, mucosal leukoplakia, nail dystrophy, and progressive bone marrow failure in most cases. Mutations in this gene also cause Hoyeraal-Hreidarsson syndrome, which is a more severe form of dyskeratosis congenita. Two transcript variants encoding different isoforms have been found for this gene.
DKC1 Antibody (PA5-25393) in WB DKC1_Antibody_PA5-25393_WB_1_112013.jpg
Western Blot with anti-DKC1 Polyclonal Antibody (PA5-25393)
Immunofluorescent analysis of 293 cells using anti-DKC1 Polyclonal Antibody (PA5-25393) at a dilution of 1:10-50. Primary antibody was detected with goat anti-rabbit lgG, Alexa Fluor 488 conjugated secondary antibody (green). DAPI was used to stain the cells nuclear (blue).
DKC1 Antibody (PA5-25393) in IHC DKC1_Antibody_PA5-25393_IHC_1_112013.jpg
Immunohistochemistry with anti-DKC1 Polyclonal Antibody (PA5-25393)
Immunohistochemistry analysis in formalin fixed and paraffin embedded human prostate carcinoma using anti-DKC1 Polyclonal Antibody (PA5-25393), followed by peroxidase conjugation of the secondary antibody and DAB staining.
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