DAP3 Antibody

DAP3 Polyclonal Antibody for Western blot

>> See 4 other antibodies for DAP3 (DAP-3, DKFZp686G12159, MGC126058, MGC126059, MRP-S29, MRPS29, bMRP-10)
Synonyms:
DAP-3, DKFZp686G12159, MGC126058, MGC126059, MRP-S29, MRPS29, bMRP-10
Entrez Gene ID:
UniProt ID:
Details
Host / Isotype: Rabbit / IgG
Class: Polyclonal
Type: Antibody
Tested Species Reactivity: Human (Hu), Mouse (Ms), Rat (Rt)
Immunogen: Synthetic peptide corresponding to residues 44-57 (T(44) S D S D P A K H G E Q H E(57) of mouse DAP-3.
Ordering Information
Pierce DAP3 Antibody
Product #
PA1-41658
Size
100 µg
Price
$285.00
Purchase
Add DAP3 Antibody to your cart.
Tested Applications Dilution *
Western Blot (WB) 1-2 µg/ml
* Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own experiment using appropriate negative and positive controls.
Form Information
Form: Liquid
Concentration: 0.5mg/ml
Purification: Protein G
Storage Buffer: PBS
Preservative: 0.02% sodium azide
Storage Conditions: Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Product Specific Information
Suggested positive control: Jurkat, antigen standard for DAP3 (transient overexpression lysate), Jurkat whole cell lysate.
General Information
Apoptosis, or programmed cell death, occurs during normal cellular differentiation and development of multicellular organisms. Recently, five novel apoptotic genes (named DAP genes for: Death Associated Proteins) have been identified that code for proteins, which display a diverse spectrum of biochemical activities. These include a novel type of calcium/calmodulin-regulated kinase which carries ankyrin repeats and a death domain (DAP-kinase), a nucleotide-binding protein (DAP-3), a small proline-rich cytoplasmic protein (DAP-1), and a novel homolog of the eIF4G translation initiation factor (DAP-5) (4). DAP-3 has an ATP/GTP binding motive (P-loop) and has been isolated from human and mouse, and named as hDAP-3 and mDAP-3 (Death Associated Protein-3), respectively (2-3). Sequence analysis revealed an 82% amino acid identity between human and mouse protein. In situ hybridization and northern blot analysis showed an abundant mRNA expression with a pronounced expression in highly proliferative epithelial compartments. Overexpression of DAP-3 leads to cytochrome c release and induction of cell death. DAP-3 mediated apoptosis was shown to depend on a functional P-loop. mDAP-3 is localized to the mitochondrial matrix and in contrast to cytochrome c, retained its mitochondrial localization during apoptosis induction (2).
Product Images
  • DAP3 Antibody (PA1-41658) in WB
    DAP3_Antibody_PA1-41658_WesternBlot(WB).jpg

    Western Blot with anti-DAP3 Polyclonal Antibody (PA1-41658) (DAP3_Antibody_PA1-41658_WesternBlot(WB).jpg)

    Western Blot with anti-DAP3 Polyclonal Antibody (PA1-41658)

  • DAP3 Antibody (PA1-41658) in WB
    anti-DAP3_Polyclonal_Antibody_(PA1-41658)_WB.jpg

    Western Blot with anti-DAP3 Polyclonal Antibody (PA1-41658) (anti-DAP3_Polyclonal_Antibody_(PA1-41658)_WB.jpg)

    Western Blot with anti-DAP3 Polyclonal Antibody (PA1-41658)

    Western blot analysis of Jurkat cells (10 ug of cell lysate) probed with a DAP3 polyclonal antibody (Product # PA1-41658) at 1 ug/ml.
(This product is for In Vitro experimental use only. Not for resale without express authorization.)
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