* Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own experiment using appropriate negative and positive controls.
0.02% sodium azide
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Product Specific Information
Suggested positive control: Jurkat, antigen standard for DAP3 (transient overexpression lysate), Jurkat whole cell lysate.
Apoptosis, or programmed cell death, occurs during normal cellular differentiation and development of multicellular organisms. Recently, five novel apoptotic genes (named DAP genes for: Death Associated Proteins) have been identified that code for proteins, which display a diverse spectrum of biochemical activities. These include a novel type of calcium/calmodulin-regulated kinase which carries ankyrin repeats and a death domain (DAP-kinase), a nucleotide-binding protein (DAP-3), a small proline-rich cytoplasmic protein (DAP-1), and a novel homolog of the eIF4G translation initiation factor (DAP-5) (4). DAP-3 has an ATP/GTP binding motive (P-loop) and has been isolated from human and mouse, and named as hDAP-3 and mDAP-3 (Death Associated Protein-3), respectively (2-3). Sequence analysis revealed an 82% amino acid identity between human and mouse protein. In situ hybridization and northern blot analysis showed an abundant mRNA expression with a pronounced expression in highly proliferative epithelial compartments. Overexpression of DAP-3 leads to cytochrome c release and induction of cell death. DAP-3 mediated apoptosis was shown to depend on a functional P-loop. mDAP-3 is localized to the mitochondrial matrix and in contrast to cytochrome c, retained its mitochondrial localization during apoptosis induction (2).
DAP3 Antibody (PA1-41658) in WB DAP3_Antibody_PA1-41658_WesternBlot(WB).jpg
Western Blot with anti-DAP3 Polyclonal Antibody (PA1-41658)