Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C
100 µg protein G purified antibody (0.5 mg/ml) in PBS with 0.02% sodium azide.
Western Blot (WB)
* Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own experiment using appropriate negative and positive controls.
Product Specific Information
PA1-41658 detects DAP3 in human, rat and murine samples.
PA1-41658 has been successfully used in Western blot applications. Positive control of Jurkat suggested.
The PA1-41658 immunogen is a synthetic peptide corresponding to residues 44-57 (T(44) S D S D P A K H G E Q H E(57) of mouse DAP-3.
Store at 4ºC, stable for 6 months. For long-term storage, store at -20ºC.
Apoptosis, or programmed cell death, occurs during normal cellular differentiation and development of multicellular organisms. Recently, five novel apoptotic genes (named DAP genes for: Death Associated Proteins) have been identified that code for proteins, which display a diverse spectrum of biochemical activities. These include a novel type of calcium/calmodulin-regulated kinase which carries ankyrin repeats and a death domain (DAP-kinase), a nucleotide-binding protein (DAP-3), a small proline-rich cytoplasmic protein (DAP-1), and a novel homolog of the eIF4G translation initiation factor (DAP-5) (4). DAP-3 has an ATP/GTP binding motive (P-loop) and has been isolated from human and mouse, and named as hDAP-3 and mDAP-3 (Death Associated Protein-3), respectively (2-3). Sequence analysis revealed an 82% amino acid identity between human and mouse protein. In situ hybridization and northern blot analysis showed an abundant mRNA expression with a pronounced expression in highly proliferative epithelial compartments. Overexpression of DAP-3 leads to cytochrome c release and induction of cell death. DAP-3 mediated apoptosis was shown to depend on a functional P-loop. mDAP-3 is localized to the mitochondrial matrix and in contrast to cytochrome c, retained its mitochondrial localization during apoptosis induction (2).
DAP3 Antibody (PA1-41658) in WB
Western Blot with anti-DAP3 Polyclonal Antibody (PA1-41658)