Cytokeratin 6 Antibody (LHK6B)

Cytokeratin 6 Monoclonal Antibody for FN

>> See 6 other antibodies for KRT6A (cytokeratin 6A, cytokeratin 6C, cytokeratin 6D, keratin, epidermal type II, K6A, keratin, type II cytoskeletal 6A, type-II keratin Kb6)
Synonyms:
cytokeratin 6A, cytokeratin 6C, cytokeratin 6D, keratin, epidermal type II, K6A, keratin, type II cytoskeletal 6A, type-II keratin Kb6
Entrez Gene ID:
UniProt ID:
Details
Host / Isotype: Mouse / IgG2a
Class: Monoclonal
Type: Antibody
Clone: LHK6B
Tested Species Reactivity: Human (Hu), Mouse (Ms)
Published Species Reactivity: Human (Hu), Mouse (Ms)
Immunogen: A synthetic peptide (GSSTIKYTTTS) from the C-terminus of human keratin 6
Ordering Information
Pierce Cytokeratin 6 Antibody (LHK6B)
Product #
MA5-14127
Size
500 ul
Price
$185.00
Purchase
Add Cytokeratin 6 Antibody (LHK6B) to your cart.
Tested Applications Dilution *
Functional Assay (FN) Assay Dependent
Published Applications Dilution
Western Blot (WB) See publications below
Immunocytochemistry (ICC) See publications below
Immunohistochemistry (IHC) See publications below
Blocking Assay (BLOCK) See publications below
* Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own experiment using appropriate negative and positive controls.
Form Information
Form: Liquid
Concentration: 0.2mg/ml
Purification: Protein A
Storage Buffer: PBS, pH 7.4, with 0.2% BSA
Preservative: 0.09% sodium azide
Storage Conditions: 4° C
Product Specific Information
MA5-14127 targets Cytokeratin 6 in FN applications and shows reactivity with Human and mouse samples.

The MA5-14127 immunogen is a synthetic peptide (GSSTIKYTTTS) from the C-terminus of human keratin 6.
General Information
Keratins 6 and 16 are expressed in keratinocytes, which are undergoing rapid turnover in the suprabasal region (also known as hyperproliferation-related keratins). Keratin 6 is found in hair follicles, suprabasal cells of a variety of internal stratified epithelia, in epidermis, in both normal and hyperproliferative situations. Epidermal injury results in activation of keratinocytes which express CK6 and CK16. CK6 is strongly expressed in about 75% of head and neck squamous cell carcinomas. Expression of CK6 is particularly associated with differentiation.
Publications:
Western Blot
  Species / Dilution Summary
  Hu / Not Cited
MA5-14127 was used in immunocytochemistry, immunohistochemistry, and western blot to investigate the relation between chronic obstructive pulmonary disease and squamous metaplasia

J Clin Invest. 2007 Nov;117(11):3551-62.
"Squamous metaplasia amplifies pathologic epithelial-mesenchymal interactions in COPD patients."
Author(s): Araya J, Cambier S, Markovics JA, Wolters P, Jablons D, Hill A, Finkbeiner W, Jones K, Broaddus VC, Sheppard D, Barzcak A, Xiao Y, Erle DJ, Nishimura SL
Number of Citations: 20
(See PubMed article )
  Ms / 0
MA5-14127 was used in immunohistochemistry and western blot to investigate the phosphorylation in type II keratins during stress and mitosis

Mol Biol Cell. 2002 Jun;13(6):1857-70.
"Type II keratins are phosphorylated on a unique motif during stress and mitosis in tissues and cultured cells."
Author(s): Toivola DM, Zhou Q, English LS, Omary MB
Number of Citations: 1
(See PubMed article )
  Ms / 1:200
MA5-14127 was used in western blot to study the effect on the morphology of head and neck squamous cell carcinoma cells of EGF-mediated regulation of cytokeratin expression

Cell Tissue Res. 2013 Jan;351(1):59-72.
"Epidermal growth factor-induced modulation of cytokeratin expression levels influences the morphological phenotype of head and neck squamous cell carcinoma cells."
Author(s): Makarova G, Bette M, Schmidt A, Jacob R, Cai C, Rodepeter F, Betz T, Sitterberg J, Bakowsky U, Moll R, Neff A, Sesterhenn A, Teymoortash A, Ocker M, Werner JA, Mandic R
Number of Citations: 0
(See PubMed article )
Immunocytochemistry
  Species / Dilution Summary
  Hu / Not Cited
MA5-14127 was used in immunocytochemistry, immunohistochemistry, and western blot to investigate the relation between chronic obstructive pulmonary disease and squamous metaplasia

J Clin Invest. 2007 Nov;117(11):3551-62.
"Squamous metaplasia amplifies pathologic epithelial-mesenchymal interactions in COPD patients."
Author(s): Araya J, Cambier S, Markovics JA, Wolters P, Jablons D, Hill A, Finkbeiner W, Jones K, Broaddus VC, Sheppard D, Barzcak A, Xiao Y, Erle DJ, Nishimura SL
Number of Citations: 20
(See PubMed article )
Immunohistochemistry
  Species / Dilution Summary
  Hu / Not Cited
MA5-14127 was used in immunohistochemistry to study the regulation of epithelial cell differentiation and human thymic medulla Hassall body formation by corticosteroids

J Immunol. 2004 Jan 1;172(1):617-24.
"Corticosteroids regulate epithelial cell differentiation and Hassall body formation in the human thymus."
Author(s): Hale LP, Markert ML
Number of Citations: 7
(See PubMed article )
  Hu / Not Cited
MA5-14127 was used in immunohistochemistry to study human X-linked severe combined immunodeficiency and thymic dendritic and epithelial cell development

Clin Immunol. 2004 Jan;110(1):63-70.
"Abnormal development of thymic dendritic and epithelial cells in human X-linked severe combined immunodeficiency."
Author(s): Hale LP, Buckley RH, Puck JM, Patel DD
Number of Citations: 4
(See PubMed article )
  Hu / 0
MA5-14127 was used in immunohistochemistry to investigate the expression profile of cytokeratins in cutaneous amyloids

Arch Dermatol Res. 2004 Jul;296(2):83-8.
"A study of cytokeratin profiles in localized cutaneous amyloids."
Author(s): Chang YT, Liu HN, Wang WJ, Lee DD, Tsai SF
Number of Citations: 1
(See PubMed article )
  Hu / 1:100
MA5-14127 was used in immunohistochemistry to develop a living skin-equivalent from de-epithelialized amnion membrane

Cell Tissue Res. 2006 Oct;326(1):69-77.
"New skin-equivalent model from de-epithelialized amnion membrane."
Author(s): Yang L, Shirakata Y, Shudou M, Dai X, Tokumaru S, Hirakawa S, Sayama K, Hamuro J, Hashimoto K
Number of Citations: 2
(See PubMed article )
  Hu / Not Cited
MA5-14127 was used in immunocytochemistry, immunohistochemistry, and western blot to investigate the relation between chronic obstructive pulmonary disease and squamous metaplasia

J Clin Invest. 2007 Nov;117(11):3551-62.
"Squamous metaplasia amplifies pathologic epithelial-mesenchymal interactions in COPD patients."
Author(s): Araya J, Cambier S, Markovics JA, Wolters P, Jablons D, Hill A, Finkbeiner W, Jones K, Broaddus VC, Sheppard D, Barzcak A, Xiao Y, Erle DJ, Nishimura SL
Number of Citations: 20
(See PubMed article )
  Hu / Not Cited
MA5-14127 was used in immunohistochemistry to study the effects of an HIV protease inhibitor on the gingival epithelium.

HIV Med. 2011 Mar;12(3):145-56.
"The HIV protease inhibitor lopinavir/ritonavir (Kaletra) alters the growth, differentiation and proliferation of primary gingival epithelium."
Author(s): Israr M, Mitchell D, Alam S, Dinello D, Kishel JJ, Meyers C
Number of Citations: 0
(See PubMed article )
  Hu / Not Cited
MA5-14127 was used in immunohistochemistry to develop and evaluate a mouse model for pachyonychia congenita

J Invest Dermatol. 2011 May;131(5):1053-60.
"Development of skin-humanized mouse models of pachyonychia congenita."
Author(s): García M, Larcher F, Hickerson RP, Baselga E, Leachman SA, Kaspar RL, Del Rio M
Number of Citations: 1
(See PubMed article )
  Hu / 0
MA5-14127 was used in immunohistochemistry to study the expression of keratin 4 and keratin 13 in oral squamous cell carcinoma and epithelial dysplasia

Histopathology. 2011 Mar;58(4):531-42.
"Down-regulation of keratin 4 and keratin 13 expression in oral squamous cell carcinoma and epithelial dysplasia: a clue for histopathogenesis."
Author(s): Sakamoto K, Aragaki T, Morita K, Kawachi H, Kayamori K, Nakanishi S, Omura K, Miki Y, Okada N, Katsube K, Takizawa T, Yamaguchi A
Number of Citations: 1
(See PubMed article )
  Hu / 10 ng/mL
MA5-14127 was used in immunohistochemistry to investigate the influence of zidovudine on gingival epithelium growth

HIV Med. 2012 May;13(5):276-90.
"Effect of the HIV nucleoside reverse transcriptase inhibitor zidovudine on the growth and differentiation of primary gingival epithelium."
Author(s): Mitchell D, Israr M, Alam S, Kishel J, Dinello D, Meyers C
Number of Citations: 1
(See PubMed article )
  Hu / 1:1000
MA5-14127 was used in immunohistochemistry to use bioengineering techniques to develop a murine skin humanized psoriasis model

Methods Mol Biol. 2013;961():305-23.
"Bioengineered skin humanized model of psoriasis."
Author(s): Carretero M, Guerrero-Aspizua S, Del Río M
Number of Citations: 0
(See PubMed article )
  Ms / 0
MA5-14127 was used in immunohistochemistry and western blot to investigate the phosphorylation in type II keratins during stress and mitosis

Mol Biol Cell. 2002 Jun;13(6):1857-70.
"Type II keratins are phosphorylated on a unique motif during stress and mitosis in tissues and cultured cells."
Author(s): Toivola DM, Zhou Q, English LS, Omary MB
Number of Citations: 1
(See PubMed article )
Blocking Assay
  Species / Dilution Summary
  Hu / Not Cited
MA5-14127 was used in blocking or activating experiment to study the role of Ku70/80 and DNA-PK in regulating RAG-mediated cleavage and the significance for the 12/23 rule specificity

J Biol Chem. 2004 Jul 9;279(28):29821-31.
"Ku70/Ku80 and DNA-dependent protein kinase catalytic subunit modulate RAG-mediated cleavage: implications for the enforcement of the 12/23 rule."
Author(s): Sawchuk DJ, Mansilla-Soto J, Alarcon C, Singha NC, Langen H, Bianchi ME, Lees-Miller SP, Nussenzweig MC, Cortes P
Number of Citations: 5
(See PubMed article )
(This product is for In Vitro experimental use only. Not for resale without express authorization.)
Part of Thermo Fisher Scientific