Cytokeratin 4 Antibody (6B10)

Cytokeratin 4 Monoclonal Antibody for Western blot, ICC, FACS, IP

>> See 6 other antibodies for KRT4 (Keratin, type II cytoskeletal 4, CK-4, Keratin-4, K4)
Synonyms:
Keratin, type II cytoskeletal 4, CK-4, Keratin-4, K4
UniProt ID:
Details
Host / Isotype: Mouse / IgG1
Class: Monoclonal
Type: Antibody
Clone: 6B10
Tested Species Reactivity: Human (Hu), Canine (Ca), Feline (Fe), Zebrafish (Zf)
Published Species Reactivity: Human (Hu), Mouse (Ms)
Immunogen: Cytokeratin preparation extracted from human esophagus.
Ordering Information
Pierce Cytokeratin 4 Antibody (6B10)
Product #
MA1-06313
Size
100 µg
Price
$280.00
Purchase
Add Cytokeratin 4 Antibody (6B10) to your cart.
Tested Applications Dilution *
Western Blot (WB) 1:100-1:1000
Immunocytochemistry (ICC) Assay dependent
Flow Cytometry (FACS) 1:25-1:200
Immunoprecipitation (IP) Assay Dependent
Published Applications Dilution
Western Blot (WB) See publications below
Immunohistochemistry (IHC) See publications below
* Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own experiment using appropriate negative and positive controls.
Form Information
Form: Liquid
Concentration: 1mg/ml
Purification: purified
Storage Buffer: PBS
Preservative: 0.09% sodium azide
Storage Conditions: 4°C or -20°C if preferred
Product Specific Information
MA1-06313 detects cytokeratin 4 in human, canine, zebrafish, and feline samples.

MA1-06313 has sucessfully been used in Western blotting, flow cytometry, immunocytochemistry, and immunoprecipitation.

The MA1-06313 immunogen is a cytokeratin preparation extracted from human esophagus. 
General Information
Cytokeratins are a subfamily of intermediate filament proteins and are characterized by a remarkable biochemical diversity, represented in human epithelial tissues by at least 20 different polypeptides. They range in molecular weight between ~40 kDa and ~68 kDa and isoelectric pH between 4.9 - 7.8. The individual human cytokeratins are numbered 1 to 20. The various epithelia in the human body usually express cytokeratins which are not only characteristic of the type of epithelium, but also related to the degree of maturation or differentiation within an epithelium. Cytokeratin subtype expression patterns are used to an increasing extent in the distinction of different types of epithelial malignancies. The cytokeratin antibodies are not only of assistance in the differential diagnosis of tumors using immunohistochemistry on tissue sections, but are also a useful tool in cytopathology and flow cytometric assays.
Publications:
Western Blot
  Species / Dilution Summary
  Ms / 0
MA1-06313 was used in immunohistochemistry and western blot to investigate the phosphorylation in type II keratins during stress and mitosis

Mol Biol Cell. 2002 Jun;13(6):1857-70.
"Type II keratins are phosphorylated on a unique motif during stress and mitosis in tissues and cultured cells."
Author(s): Toivola DM, Zhou Q, English LS, Omary MB
Number of Citations: 1
(See PubMed article )
Immunohistochemistry
  Species / Dilution Summary
  Hu / 1:50
MA1-06313 was used in immunohistochemistry to study the role of interactions with regionally defined fibroblast populations on the phenotypes of gingival epithelial cells using an organotypic model

J Periodontal Res. 2008 Jun;43(3):279-89.
"Modulation of gingival epithelial phenotypes by interactions with regionally defined populations of fibroblasts."
Author(s): Locke M, Hyland PL, Irwin CR, Mackenzie IC
Number of Citations: 1
(See PubMed article )
  Hu / 0
MA1-06313 was used in immunohistochemistry to study the expression pattern of stem cells in the bulbar conjunctival epithelium

J Cell Physiol. 2010 Oct;225(1):180-5.
"Potential localization of putative stem/progenitor cells in human bulbar conjunctival epithelium."
Author(s): Qi H, Zheng X, Yuan X, Pflugfelder SC, Li DQ
Number of Citations: 1
(See PubMed article )
  Ms / 0
MA1-06313 was used in immunohistochemistry and western blot to investigate the phosphorylation in type II keratins during stress and mitosis

Mol Biol Cell. 2002 Jun;13(6):1857-70.
"Type II keratins are phosphorylated on a unique motif during stress and mitosis in tissues and cultured cells."
Author(s): Toivola DM, Zhou Q, English LS, Omary MB
Number of Citations: 1
(See PubMed article )
(This product is for In Vitro experimental use only. Not for resale without express authorization.)
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