Cytochrome P450 1A1, 1A2 Antibody (MC1)

Cytochrome P450 1A1, 1A2 Monoclonal Antibody for Western blot, IF, ICC, IHC (P)

Synonyms:
CP12, P3-450, P450(PA)
Details
Host / Isotype: Mouse / IgG1
Class: Monoclonal
Type: Antibody
Clone: MC1
Tested Species Reactivity: Human (Hu), Mouse (Ms), Rat (Rt), Non-human primate (Nhp)
Immunogen: 3-methylcholanthrene induced rat cytochrome P450 protein.
Ordering Information
Pierce Cytochrome P450 1A1, 1A2 Antibody (MC1)
Product #
MA3-036
Size
100 µl
Price
$316.00
Purchase
Add Cytochrome P450 1A1, 1A2 Antibody (MC1) to your cart.
Tested Applications Dilution *
Western Blot (WB) 1:500
Immunofluorescence (IF) 1:20-1:200
Immunocytochemistry (ICC) 1:20-1:200
Immunohistochemistry (Paraffin) (IHC (P)) 1:50-1:200
* Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own experiment using appropriate negative and positive controls.
Form Information
Form: Liquid
Storage Buffer: ascites
Preservative: 0.05% sodium azide
Storage Conditions: -20° C, Avoid Freeze/Thaw Cycles
Product Specific Information
MA3-036 detects Cytochrome P450 1A1/1A2 from human, primate, rat and mouse samples.

MA3-036 has been successfully used in Western blot, immunocytochemistry, immunofluorescence and immunohistochemisty (paraffin) applications. By Western blot, this antibody detects a ~58 kDa band representing Cytochrome P450 1A1/1A2.

The MA3-036 immunogen is 3-methylcholanthrene induced rat cytochrome P450 protein.
General Information
This gene encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. The protein encoded by this gene localizes to the endoplasmic reticulum and its expression is induced by some polycyclic aromatic hydrocarbons (PAHs), some of which are found in cigarette smoke. The enzyme's endogenous substrate is unknown; however, it is able to metabolize some PAHs to carcinogenic intermediates. Other xenobiotic substrates for this enzyme include caffeine, aflatoxin B1, and acetaminophen. The transcript from this gene contains four Alu sequences flanked by direct repeats in the 3' untranslated region.
Product Images
  • Cytochrome P450 1A1, 1A2 Antibody (MA3-036) in IF
    MA3-036_IF_Hela_20130724073001.jpg

    Immunofluorescence with anti-Cytochrome P450 1A1, 1A2 Monoclonal Antibody [MC1] (MA3-036) (MA3-036_IF_Hela_20130724073001.jpg)

    Immunofluorescence with anti-Cytochrome P450 1A1, 1A2 Monoclonal Antibody [MC1] (MA3-036)

    Immunofluorescent analysis of Cytochrome P450 1A1/1A2 (green) showing staining in the cytoplasm and membrane of HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with a Cytochrome P450 1A1/1A2 monoclonal antibody (Product # MA3-036) in 3% BSA-PBS at a dilution of 1:100 and incubated overnight at 4 ºC in a humidified chamber. Cells were washed with PBST and incubated with a DyLight-conjugated secondary antibody in PBS at room temperature in the dark. F-actin (red) was stained with a flourescent red phalloidin and nuclei (blue) were stained with Hoechst or DAPI. Images were taken at a magnification of 60x.
  • Cytochrome P450 1A1, 1A2 Antibody (MA3-036) in IF
    MA3-036_IF_COS-7_20130724073022.jpg

    Immunofluorescence with anti-Cytochrome P450 1A1, 1A2 Monoclonal Antibody [MC1] (MA3-036) (MA3-036_IF_COS-7_20130724073022.jpg)

    Immunofluorescence with anti-Cytochrome P450 1A1, 1A2 Monoclonal Antibody [MC1] (MA3-036)

    Immunofluorescent analysis of Cytochrome P450 1A1/1A2 (green) showing staining in the cytoplasm and membrane of COS-7 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with a Cytochrome P450 1A1/1A2 monoclonal antibody (Product # MA3-036) in 3% BSA-PBS at a dilution of 1:100 and incubated overnight at 4 ºC in a humidified chamber. Cells were washed with PBST and incubated with a DyLight-conjugated secondary antibody in PBS at room temperature in the dark. F-actin (red) was stained with a flourescent red phalloidin and nuclei (blue) were stained with Hoechst or DAPI. Images were taken at a magnification of 60x.
  • Cytochrome P450 1A1, 1A2 Antibody (MA3-036) in IF
    MA3-036_IF_HT29_20130724073046.jpg

    Immunofluorescence with anti-Cytochrome P450 1A1, 1A2 Monoclonal Antibody [MC1] (MA3-036) (MA3-036_IF_HT29_20130724073046.jpg)

    Immunofluorescence with anti-Cytochrome P450 1A1, 1A2 Monoclonal Antibody [MC1] (MA3-036)

    Immunofluorescent analysis of Cytochrome P450 1A1/1A2 (green) showing staining in the cytoplasm and membrane of HT29 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with a Cytochrome P450 1A1/1A2 monoclonal antibody (Product # MA3-036) in 3% BSA-PBS at a dilution of 1:100 and incubated overnight at 4 ºC in a humidified chamber. Cells were washed with PBST and incubated with a DyLight-conjugated secondary antibody in PBS at room temperature in the dark. F-actin (red) was stained with a flourescent red phalloidin and nuclei (blue) were stained with Hoechst or DAPI. Images were taken at a magnification of 60x.
  • Cytochrome P450 1A1, 1A2 Antibody (MA3-036) in IHC (P)
    MA3-036_IHC_Human_liver_tissue_20130613141306.jpg

    Immunohistochemistry (Paraffin) with anti-Cytochrome P450 1A1, 1A2 Monoclonal Antibody [MC1] (MA3-036) (MA3-036_IHC_Human_liver_tissue_20130613141306.jpg)

    Immunohistochemistry (Paraffin) with anti-Cytochrome P450 1A1, 1A2 Monoclonal Antibody [MC1] (MA3-036)

    Immunohistochemistry analysis of Cytochrome P450 1A1/1A2 showing positive staining in the cytoplasm and membrane of paraffin-treated Human liver tissue (right) compared with a negative control in the absence of primary antibody (left). To expose target proteins, antigen retrieval method was performed using 10mM sodium citrate (pH 6.0) microwaved for 8-15 min. Following antigen retrieval, tissues were blocked in 3% H2O2-methanol for 15 min at room temperature, washed with ddH2O and PBS, and then probed with a Cytochrome P450 1A1/1A2 monoclonal antibody (Product # MA3-036) diluted by 3% BSA-PBS at a dilution of 1:100 overnight at 4°C in a humidified chamber. Tissues were washed extensively PBST and detection was performed using an HRP-conjugated secondary antibody followed by colorimetric detection using a DAB kit. Tissues were counterstained with hematoxylin and dehydrated with ethanol and xylene to prep for mounting.
  • Cytochrome P450 1A1, 1A2 Antibody (MA3-036) in IHC (P)
    MA3-036_IHC_Human_prostate_tissue_20130613141336.jpg

    Immunohistochemistry (Paraffin) with anti-Cytochrome P450 1A1, 1A2 Monoclonal Antibody [MC1] (MA3-036) (MA3-036_IHC_Human_prostate_tissue_20130613141336.jpg)

    Immunohistochemistry (Paraffin) with anti-Cytochrome P450 1A1, 1A2 Monoclonal Antibody [MC1] (MA3-036)

    Immunohistochemistry analysis of Cytochrome P450 1A1/1A2 showing positive staining in the membrane of paraffin-treated Human prostate tissue (right) compared with a negative control in the absence of primary antibody (left). To expose target proteins, antigen retrieval method was performed using 10mM sodium citrate (pH 6.0) microwaved for 8-15 min. Following antigen retrieval, tissues were blocked in 3% H2O2-methanol for 15 min at room temperature, washed with ddH2O and PBS, and then probed with a Cytochrome P450 1A1/1A2 monoclonal antibody (Product # MA3-036) diluted by 3% BSA-PBS at a dilution of 1:100 overnight at 4°C in a humidified chamber. Tissues were washed extensively PBST and detection was performed using an HRP-conjugated secondary antibody followed by colorimetric detection using a DAB kit. Tissues were counterstained with hematoxylin and dehydrated with ethanol and xylene to prep for mounting.
  • Cytochrome P450 1A1, 1A2 Antibody (MA3-036) in IHC (P)
    MA3-036_IHC_Mouse_liver_tissue_20130613141406.jpg

    Immunohistochemistry (Paraffin) with anti-Cytochrome P450 1A1, 1A2 Monoclonal Antibody [MC1] (MA3-036) (MA3-036_IHC_Mouse_liver_tissue_20130613141406.jpg)

    Immunohistochemistry (Paraffin) with anti-Cytochrome P450 1A1, 1A2 Monoclonal Antibody [MC1] (MA3-036)

    Immunohistochemistry analysis of Cytochrome P450 1A1/1A2 showing positive staining in the cytoplasm and membrane of paraffin-treated Mouse liver tissue (right) compared with a negative control in the absence of primary antibody (left). To expose target proteins, antigen retrieval method was performed using 10mM sodium citrate (pH 6.0) microwaved for 8-15 min. Following antigen retrieval, tissues were blocked in 3% H2O2-methanol for 15 min at room temperature, washed with ddH2O and PBS, and then probed with a Cytochrome P450 1A1/1A2 monoclonal antibody (Product # MA3-036) diluted by 3% BSA-PBS at a dilution of 1:100 overnight at 4°C in a humidified chamber. Tissues were washed extensively PBST and detection was performed using an HRP-conjugated secondary antibody followed by colorimetric detection using a DAB kit. Tissues were counterstained with hematoxylin and dehydrated with ethanol and xylene to prep for mounting.
  • Cytochrome P450 1A1, 1A2 Antibody (MA3-036) in IHC (P)
    MA3-036_IHC_rat_liver_tissue.jpg

    Immunohistochemistry (Paraffin) with anti-Cytochrome P450 1A1, 1A2 Monoclonal Antibody [MC1] (MA3-036) (MA3-036_IHC_rat_liver_tissue.jpg)

    Immunohistochemistry (Paraffin) with anti-Cytochrome P450 1A1, 1A2 Monoclonal Antibody [MC1] (MA3-036)

    Immunohistochemistry analysis of Cytochrome P450 showing staining in the cytoplasm and membrane of paraffin-treated rat liver tissue (right) compared with a negative control without primary antibody (left). To expose target proteins, antigen retrieval was performed using 10mM sodium citrate (pH 6.0), microwaved for 8-15 min. Following antigen retrieval, tissues were blocked in 3% H2O2-methanol for 15 min at room temperature, washed with ddH2O and PBS, and then probed with a Cytochrome P450 1A1/1A2 monoclonal antibody (Product # MA3-036) diluted by 3% BSA-PBS at a dilution of 1:100 overnight at 4°C in a humidified chamber. Tissues were washed extensively in PBST and detection was performed using an HRP-conjugated secondary antibody followed by colorimetric detection using a DAB kit. Tissues were counterstained with hematoxylin and dehydrated with ethanol and xylene to prep for mounting.
(This product is for In Vitro experimental use only. Not for resale without express authorization.)
Part of Thermo Fisher Scientific