* Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own experiment using appropriate negative and positive controls.
Antigen affinity chromatography
0.05% sodium azide
-20° C, Avoid Freeze/Thaw Cycles
Product Specific Information
PA1-776 detects Cysteine String Protein Alpha in mouse and rat brain samples.
PA1-776 has been successfully used in Western blot procedures. By Western blot, this antibody detects a ~34 kDa protein representing Cysteine String Protein Alpha in rat and mouse brain homogenates.
The PA1-776 immunogen is a synthetic peptide corresponding to residues E(180) T T Q L T A D S H P S Y H T D G F N(198) of rat CSP. This sequence is conserved across many species including mouse, human and bovine. This peptide (Cat. # PEP-287) is available for use in neutralization and control experiments.
Cysteine string protein alpha (CSPalpha) is a synaptic vesicle protein that contains a DNA-J domain characteristic of Hsp40 chaperones. Cysteine-string proteins are membrane-bound proteins associated primarily with nerve endings and synaptic vesicles in the brain. These proteins play an important role in presynaptic function and may be involved in calcium-dependent neurotransmitter release. CSP proteins may act as presynaptic chaperones that assist in maintaining synaptic function.
Cysteine String Protein alpha Antibody (PA1-776) in WB PA1-776_1_07232010.jpg
Western Blot with anti-Cysteine String Protein alpha Polyclonal Antibody (PA1-776)