Cyclophilin B Antibody

Cyclophilin B Polyclonal Antibody for Western blot, IF, ICC, IHC (P)

>> See 2 other antibodies for PPIB (Cyp B, Peptidyl-prolyl cis-trans isomerase B, EC 5.2.1.8, PPIase, Rotamase, S-cyclophilin, SCYLP, CYP-S1)
Synonyms:
Cyp B, Peptidyl-prolyl cis-trans isomerase B, EC 5.2.1.8, PPIase, Rotamase, S-cyclophilin, SCYLP, CYP-S1
Details
Host / Isotype: Rabbit
Class: Polyclonal
Type: Antibody
Tested Species Reactivity: Human (Hu), Mouse (Ms), Rat (Rt), Canine (Ca)
Published Species Reactivity: Guinea Pig (GP), Human (Hu), Mouse (Ms), Rat (Rt)
Immunogen: Synthetic peptide corresponding to residues C(194) G K I E V E K P F A I A K E(208) of human CyPB.
Ordering Information
Pierce Cyclophilin B Antibody
Product #
PA1-027A
Size
100 µg
Price
$199.00
Purchase
Add Cyclophilin B Antibody to your cart.
Tested Applications Dilution *
Western Blot (WB) 0.5 µg/ml
Immunofluorescence (IF) 1:100-1:1000
Immunocytochemistry (ICC) 1:100-1:1000
Immunohistochemistry (Paraffin) (IHC (P)) 1:50-1:500
Published Applications Dilution
Western Blot (WB) See publications below
Immunohistochemistry (IHC) See publications below
Immunoprecipitation (IP) See publications below
* Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own experiment using appropriate negative and positive controls.
Form Information
Form: Liquid
Concentration: 1mg/ml
Purification: Antigen affinity chromatography
Storage Buffer: PBS with 1mg/ml BSA
Preservative: 0.05% sodium azide
Storage Conditions: -20° C, Avoid Freeze/Thaw Cycles
Product Specific Information
PA1-027A detects cyclophilin B (CyPB) in mouse, rat, canine, and human tissues.

PA1-027A has been successfully used in Western blot procedures, IF/ICC and IHC (P). By Western blot, this antibody detects a ~19 kDa protein representing CyPB from rat liver extract.

The PA1-027A immunogen is a synthetic peptide corresponding to residues C(194) G K I E V E K P F A I A K E(208) of human CyPB. This sequence is almost completely conserved between human, mouse, rat, bovine, and chicken. PA1-027A immunizing peptide (Cat. # PEP-058) is available for use in neutralization and control experiments.
General Information
Immunophilins are a family of soluble cytosolic receptors capable of binding to one of two major immunosuppressant agents - cyclosporin A (CsA) or FK506. Proteins which bind FK506 are termed FK506 Binding Proteins (FKBP's) and those which bind cyclosporin A are called cyclophilins (CyP).

Both CyP:CsA and FKBP:FK506 complexes have been shown to inhibit calcineurin, a calcium and calmodulin dependent protein phosphatase which has been implicated as an important signaling enzyme in T-cell activation. Thus, providing a possible mechanism of immunosuppression by CsA and FK506. Immunophilins function as peptidyl prolyl cis-trans-isomerases (PPIase) whose activity is inhibited by their respective immunosuppressant compounds. As PPIase's, immunophilins accelerate folding of some proteins both in vivo and in vitro by catalyzing slow steps in the initial folding and rearrangement of proline containing proteins.
Product Images
  • Cyclophilin B Antibody (PA1-027A) in WB
    PA1-027A_WesternBlot.jpg

    Western Blot with anti-Cyclophilin B Polyclonal Antibody (PA1-027A) (PA1-027A_WesternBlot.jpg)

    Western Blot with anti-Cyclophilin B Polyclonal Antibody (PA1-027A)

    Western blot analysis of Cyclophilin B in Rat Liver lysate (25 ug) using anti-Cyclophilin B antibody, PA1-027A at a dilution of 1:2000 (lane 2), 1:1000 (lane 3), 1:2000 (lane 4) and 1:4000 (lane 5).
  • Cyclophilin B Antibody (PA1-027A) in IF
    PA1-027A_IF_A431.jpg

    Immunofluorescence with anti-Cyclophilin B Polyclonal Antibody (PA1-027A) (PA1-027A_IF_A431.jpg)

    Immunofluorescence with anti-Cyclophilin B Polyclonal Antibody (PA1-027A)

    Immunofluorescent analysis of Cyclophilin B (green) showing staining in the in the cytoplasm of A431 cells (right) compared to a negative control without primary antibody (left). Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with a Cyclophilin B polyclonal antibody (Product # PA1-027A) in 3% BSA-PBS at a dilution of 1:500 and incubated overnight at 4ºC in a humidified chamber. Cells were washed with PBST and incubated with a DyLight-conjugated secondary antibody in PBS at room temperature in the dark. F-actin (red) was stained with a flourescent red phalloidin and nuclei (blue) were stained with Hoechst or DAPI. Images were taken at a magnification of 60x.
  • Cyclophilin B Antibody (PA1-027A) in IF
    PA1-027A_IF_HepG2.jpg

    Immunofluorescence with anti-Cyclophilin B Polyclonal Antibody (PA1-027A) (PA1-027A_IF_HepG2.jpg)

    Immunofluorescence with anti-Cyclophilin B Polyclonal Antibody (PA1-027A)

    Immunofluorescent analysis of Cyclophilin B (green) showing staining in the in the cytoplasm of HepG2 cells (right) compared to a negative control without primary antibody (left). Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with a Cyclophilin B polyclonal antibody (Product # PA1-027A) in 3% BSA-PBS at a dilution of 1:200 and incubated overnight at 4ºC in a humidified chamber. Cells were washed with PBST and incubated with a DyLight-conjugated secondary antibody in PBS at room temperature in the dark. Nuclei (blue) were stained with Hoechst or DAPI. Images were taken at a magnification of 60x.
  • Cyclophilin B Antibody (PA1-027A) in IHC (P)
    PA1-027A_IHC_human_hepatocarcinoma.jpg

    Immunohistochemistry (Paraffin) with anti-Cyclophilin B Polyclonal Antibody (PA1-027A) (PA1-027A_IHC_human_hepatocarcinoma.jpg)

    Immunohistochemistry (Paraffin) with anti-Cyclophilin B Polyclonal Antibody (PA1-027A)

    Immunohistochemistry analysis of Cyclophilin B showing staining in the cytoplasm of paraffin-embedded human hepatocarcinoma (right) compared with a negative control without primary antibody (left). To expose target proteins, antigen retrieval was performed using 10mM sodium citrate (pH 6.0), microwaved for 8-15 min. Following antigen retrieval, tissues were blocked in 3% H2O2-methanol for 15 min at room temperature, washed with ddH2O and PBS, and then probed with a Cyclophilin B polyclonal antibody (Product # PA1-027A) diluted in 3% BSA-PBS at a dilution of 1:200 overnight at 4°C in a humidified chamber. Tissues were washed extensively in PBST and detection was performed using an HRP-conjugated secondary antibody followed by colorimetric detection using a DAB kit. Tissues were counterstained with hematoxylin and dehydrated with ethanol and xylene to prep for mounting.
  • Cyclophilin B Antibody (PA1-027A) in IHC (P)
    PA1-027A_IHC_human_liver_tissue.jpg

    Immunohistochemistry (Paraffin) with anti-Cyclophilin B Polyclonal Antibody (PA1-027A) (PA1-027A_IHC_human_liver_tissue.jpg)

    Immunohistochemistry (Paraffin) with anti-Cyclophilin B Polyclonal Antibody (PA1-027A)

    Immunohistochemistry analysis of Cyclophilin B showing staining in the cytoplasm of paraffin-embedded human liver tissue (right) compared with a negative control without primary antibody (left). To expose target proteins, antigen retrieval was performed using 10mM sodium citrate (pH 6.0), microwaved for 8-15 min. Following antigen retrieval, tissues were blocked in 3% H2O2-methanol for 15 min at room temperature, washed with ddH2O and PBS, and then probed with a Cyclophilin B polyclonal antibody (Product # PA1-027A) diluted in 3% BSA-PBS at a dilution of 1:100 overnight at 4°C in a humidified chamber. Tissues were washed extensively in PBST and detection was performed using an HRP-conjugated secondary antibody followed by colorimetric detection using a DAB kit. Tissues were counterstained with hematoxylin and dehydrated with ethanol and xylene to prep for mounting.
  • Cyclophilin B Antibody (PA1-027A) in IHC (P)
    PA1-027A_IHC_rat_liver_tissue.jpg

    Immunohistochemistry (Paraffin) with anti-Cyclophilin B Polyclonal Antibody (PA1-027A) (PA1-027A_IHC_rat_liver_tissue.jpg)

    Immunohistochemistry (Paraffin) with anti-Cyclophilin B Polyclonal Antibody (PA1-027A)

    Immunohistochemistry analysis of Cyclophilin B showing staining in the cytoplasm of paraffin-embedded rat liver tissue (right) compared with a negative control without primary antibody (left). To expose target proteins, antigen retrieval was performed using 10mM sodium citrate (pH 6.0), microwaved for 8-15 min. Following antigen retrieval, tissues were blocked in 3% H2O2-methanol for 15 min at room temperature, washed with ddH2O and PBS, and then probed with a Cyclophilin B polyclonal antibody (Product # PA1-027A) diluted in 3% BSA-PBS at a dilution of 1:100 overnight at 4°C in a humidified chamber. Tissues were washed extensively in PBST and detection was performed using an HRP-conjugated secondary antibody followed by colorimetric detection using a DAB kit. Tissues were counterstained with hematoxylin and dehydrated with ethanol and xylene to prep for mounting.
Publications:
Western Blot
  Species / Dilution Summary
  Hu / 1:1,000
PA1-027A was used in western blot to study the effects of cyclosporin A and PSC833 on the activity and expression of the sodium/calcium exchanger NCX1

J Biol Chem. 2002 Jan 25;277(4):2505-10.
"Transport activity and surface expression of the Na+-Ca2+ exchanger NCX1 are inhibited by the immunosuppressive agent cyclosporin A and by the nonimmunosuppressive agent PSC833."
Author(s): Kimchi-Sarfaty C, Kasir J, Ambudkar SV, Rahamimoff H
Number of Citations: 1
(See PubMed article )
  Hu / 0
PA1-027A was used in western blot to investigate the inhibitory effect of calumenin on the vitamin K-dependent gamma-carboxylation system

J Biol Chem. 2004 Jun 11;279(24):25276-83.
"The inhibitory effect of calumenin on the vitamin K-dependent gamma-carboxylation system. Characterization of the system in normal and warfarin-resistant rats."
Author(s): Wajih N, Sane DC, Hutson SM, Wallin R
Number of Citations: 19
(See PubMed article )
  Hu / 1:50000
PA1-027A was used in western blot to identify factors that influence skeletal muscle PGC-1alpha and PGC-1beta gene expression

J Clin Invest. 2004 Nov;114(10):1518-26.
"Multiple environmental and genetic factors influence skeletal muscle PGC-1alpha and PGC-1beta gene expression in twins."
Author(s): Ling C, Poulsen P, Carlsson E, Ridderstråle M, Almgren P, Wojtaszewski J, Beck-Nielsen H, Groop L, Vaag A
Number of Citations: 52
(See PubMed article )
  Hu / 0
PA1-027A was used in western blot to explore the role of cyclophilin B in activation of interferon regulatory factor-3

J Biol Chem. 2005 May 6;280(18):18355-60.
"Role of cyclophilin B in activation of interferon regulatory factor-3."
Author(s): Obata Y, Yamamoto K, Miyazaki M, Shimotohno K, Kohno S, Matsuyama T
Number of Citations: 8
(See PubMed article )
  Hu / 0
PA1-027A was used in western blot to study mechanisms of mitochondrial dysfunction in propionic aciduria

Biochem J. 2006 Aug 15;398(1):107-12.
"Secondary mitochondrial dysfunction in propionic aciduria: a pathogenic role for endogenous mitochondrial toxins."
Author(s): Schwab MA, Sauer SW, Okun JG, Nijtmans LG, Rodenburg RJ, van den Heuvel LP, Dröse S, Brandt U, Hoffmann GF, Ter Laak H, Kölker S, Smeitink JA
Number of Citations: 11
(See PubMed article )
  Hu / 0
PA1-027A was used in western blot to characterize hepatitis C virus cells that are resistant to cyclosporine

J Virol. 2007 Jun;81(11):5829-40.
"Characterization of hepatitis C virus subgenomic replicon resistance to cyclosporine in vitro."
Author(s): Robida JM, Nelson HB, Liu Z, Tang H
Number of Citations: 39
(See PubMed article )
  Hu / Not Cited
PA1-027A was used in western blot to study the effect of lentiviral short-hairpin RNAs delivery on interferon pathway activation

Nucleic Acids Res. 2009 Oct;37(19):6587-99.
"Short-hairpin RNAs delivered by lentiviral vector transduction trigger RIG-I-mediated IFN activation."
Author(s): Kenworthy R, Lambert D, Yang F, Wang N, Chen Z, Zhu H, Zhu F, Liu C, Li K, Tang H
Number of Citations: 5
(See PubMed article )
  Hu / Not Cited
PA1-027A was used in western blot to investigate the role of acyl-CoA dehydrogenase 9 in oxidative phosphorylation complex I formation

Cell Metab. 2010 Sep 8;12(3):283-94.
"Acyl-CoA dehydrogenase 9 is required for the biogenesis of oxidative phosphorylation complex I."
Author(s): Nouws J, Nijtmans L, Houten SM, van den Brand M, Huynen M, Venselaar H, Hoefs S, Gloerich J, Kronick J, Hutchin T, Willems P, Rodenburg R, Wanders R, van den Heuvel L, Smeitink J, Vogel RO
Number of Citations: 9
(See PubMed article )
  Hu / Not Cited
PA1-027A was used in western blot to investigate the effect of calcineurin signaling on human islet beta-cell survival

J Biol Chem. 2010 Dec 17;285(51):40050-9.
"Calcineurin signaling regulates human islet {beta}-cell survival."
Author(s): Soleimanpour SA, Crutchlow MF, Ferrari AM, Raum JC, Groff DN, Rankin MM, Liu C, De León DD, Naji A, Kushner JA, Stoffers DA
Number of Citations: 4
(See PubMed article )
  Hu / 0
PA1-027A was used in western blot to investigate the effect of PPIB mutations on type I procollagen chain association and osteogenesis

Hum Mol Genet. 2011 Apr 15;20(8):1595-609.
"Mutations in PPIB (cyclophilin B) delay type I procollagen chain association and result in perinatal lethal to moderate osteogenesis imperfecta phenotypes."
Author(s): Pyott SM, Schwarze U, Christiansen HE, Pepin MG, Leistritz DF, Dineen R, Harris C, Burton BK, Angle B, Kim K, Sussman MD, Weis M, Eyre DR, Russell DW, McCarthy KJ, Steiner RD, Byers PH
Number of Citations: 1
(See PubMed article )
  Hu / 1:20000
PA1-027A was used in western blot to characterize the expression of glutathione S-transferase in liver

Exp Physiol. 2011 Aug;96(8):790-800.
"Liver glutathione S-transferase expression is decreased by 3,5,3-triiodothyronine in hypothyroid but not in euthyroid mice."
Author(s): Faustino LC, Pires RM, Lima AC, Cordeiro A, Souza LL, Ortiga-Carvalho TM
Number of Citations: 0
(See PubMed article )
  Ms / 0
PA1-027A was used in western blot to investigate the transcriptional regulation of keratin 19 in pancreatic ductal cellls

J Biol Chem. 2006 Dec 15;281(50):38385-95.
"The PDX1 homeodomain transcription factor negatively regulates the pancreatic ductal cell-specific keratin 19 promoter."
Author(s): Deramaudt TB, Sachdeva MM, Wescott MP, Chen Y, Stoffers DA, Rustgi AK
Number of Citations: 11
(See PubMed article )
  Ms / Not Cited
PA1-027A was used in western blot to investigate the effect of RANK signal inhibition on osteoclast function

Pharm Res. 2010 Jul;27(7):1273-84.
"siRNA knock-down of RANK signaling to control osteoclast-mediated bone resorption."
Author(s): Wang Y, Grainger DW
Number of Citations: 3
(See PubMed article )
  Ms / Not Cited
PA1-027A was used in western blot to investigate the therapeutic benefit of mTOR inhibition in the amelioration of fibrotic encapsulation

J Control Release. 2010 Nov 1;147(3):400-7.
"Device-based local delivery of siRNA against mammalian target of rapamycin (mTOR) in a murine subcutaneous implant model to inhibit fibrous encapsulation."
Author(s): Takahashi H, Wang Y, Grainger DW
Number of Citations: 1
(See PubMed article )
  Ms / 0
PA1-027A was used in western blot to evaluate a small interfering RNA against farnesyl pyrophosphate synthase

Mol Pharm. 2011 Aug 1;8(4):1016-24.
"Small interfering RNA knocks down the molecular target of alendronate, farnesyl pyrophosphate synthase, in osteoclast and osteoblast cultures."
Author(s): Wang Y, Panasiuk A, Grainger DW
Number of Citations: 1
(See PubMed article )
  Ms / 1:3000
PA1-027A was used in western blot to study the ability of blocking leptin after weaning to substantially reverse the the effects of neonatal hyperleptinaemia.

Horm Metab Res. 2011 Mar;43(3):171-7.
"Blocking leptin action one week after weaning reverts most of the programming caused by neonatal hyperleptinemia in the adult rat."
Author(s): Trotta PA, Moura EG, Franco JG, Lima NS, de Oliveira E, Cordeiro A, Souza LL, Oliveira KJ, Lisboa PC, Pazos Moura CC, Passos MC
Number of Citations: 0
(See PubMed article )
  Ms / 1:200
PA1-027A was used in western blot to study the role of myosin II activity in focal adhesion using proteomics

Nat Cell Biol. 2011 Apr;13(4):383-93.
"Analysis of the myosin-II-responsive focal adhesion proteome reveals a role for ?-Pix in negative regulation of focal adhesion maturation."
Author(s): Kuo JC, Han X, Hsiao CT, Yates JR 3rd, Waterman CM
Number of Citations: 21
(See PubMed article )
  Ms / 1:20000
PA1-027A was used in western blot to compare the effects of thyroid hormone and estradiol on kidney GST-? expression

Am J Physiol Endocrinol Metab. 2012 Sep 15;303(6):E787-97.
"Thyroid hormone and estradiol have overlapping effects on kidney glutathione S-transferase-? gene expression."
Author(s): Faustino LC, Almeida NA, Pereira GF, Ramos RG, Soares RM, Morales MM, Pazos-Moura CC, Ortiga-Carvalho TM
Number of Citations: 1
(See PubMed article )
  Rt / 1:100000
PA1-027A was used in western blot to examine the role of antioxidant enzymes in rat retinal ischemia-reperfusion injury and recovery

Metabolism. 2006 Jul;55(7):892-8.
"Expression of antioxidant enzymes in rat retinal ischemia followed by reperfusion."
Author(s): Agardh CD, Gustavsson C, Hagert P, Nilsson M, Agardh E
Number of Citations: 2
(See PubMed article )
  Rt / 1:4000
PA1-027A was used in western blot to gain insights into the toxicity mechanisms of soluble protein oligomers

J Neurochem. 2007 Oct;103(2):736-48.
"Soluble oligomers from a non-disease related protein mimic Abeta-induced tau hyperphosphorylation and neurodegeneration."
Author(s): Vieira MN, Forny-Germano L, Saraiva LM, Sebollela A, Martinez AM, Houzel JC, De Felice FG, Ferreira ST
Number of Citations: 7
(See PubMed article )
  Rt / 1:20000
PA1-027A was used in western blot to examine the effects of exendin-4 on islet vascularity in intrauterine growth restricted rats

Pediatr Res. 2009 Jul;66(1):42-6.
"Exendin-4 normalizes islet vascularity in intrauterine growth restricted rats: potential role of VEGF."
Author(s): Ham JN, Crutchlow MF, Desai BM, Simmons RA, Stoffers DA
Number of Citations: 6
(See PubMed article )
  Rt / 1:15000
PA1-027A was used in western blot to investigate the effect of protein kinase C on serine racemase and D-serine availability

J Neurochem. 2011 Jan;116(2):281-90.
"Protein kinase C activity regulates D-serine availability in the brain."
Author(s): Vargas-Lopes C, Madeira C, Kahn SA, Albino do Couto I, Bado P, Houzel JC, De Miranda J, de Freitas MS, Ferreira ST, Panizzutti R
Number of Citations: 1
(See PubMed article )
  Rt / 1:10000
PA1-027A was used in western blot to investigate the protective effect of D1/D5 receptor activation on synapse function

J Biol Chem. 2011 Feb 4;286(5):3270-6.
"Activation of D1/D5 dopamine receptors protects neurons from synapse dysfunction induced by amyloid-beta oligomers."
Author(s): Jürgensen S, Antonio LL, Mussi GE, Brito-Moreira J, Bomfim TR, De Felice FG, Garrido-Sanabria ER, Cavalheiro ÉA, Ferreira ST
Number of Citations: 2
(See PubMed article )
  Rt / 1:4000
PA1-027A was used in western blot to investigate the effects of amyloid-? on mitochondria

PLoS One. 2010 Dec 16;5(12):e15230.
"Amyloid-? triggers the release of neuronal hexokinase 1 from mitochondria."
Author(s): Saraiva LM, Seixas da Silva GS, Galina A, da-Silva WS, Klein WL, Ferreira ST, De Felice FG
Number of Citations: 5
(See PubMed article )
  Rt / 1:50000
PA1-027A was used in western blot to investigate the role of thyroid hormone signaling in the hypolipidemic effect of n-3 polyunsaturated fatty acidsn-3

J Endocrinol. 2011 Oct;211(1):65-72.
"Thyroid hormone contributes to the hypolipidemic effect of polyunsaturated fatty acids from fish oil: in vivo evidence for cross talking mechanisms."
Author(s): Souza LL, Cordeiro A, Oliveira LS, de Paula GS, Faustino LC, Ortiga-Carvalho TM, Oliveira KJ, Pazos-Moura CC
Number of Citations: 0
(See PubMed article )
Immunohistochemistry
  Species / Dilution Summary
  GP / 1:50
PA1-027A was used in immunohistochemistry to study cyclophilin B in chrondocyte pathology

Arthritis Rheum. 2003 Aug;48(8):2197-206.
"High binding capacity of cyclophilin B to chondrocyte heparan sulfate proteoglycans and its release from the cell surface by matrix metalloproteinases: possible role as a proinflammatory mediator in arthritis."
Author(s): De Ceuninck F, Allain F, Caliez A, Spik G, Vanhoutte PM
Number of Citations: 9
(See PubMed article )
  Hu / Not Cited
PA1-027A was used in immunohistochemistry to study the mechanism for the cytoplasmic accumulation of extracellular matrix proteins and chaperones associated in pseudoachondroplasia

Matrix Biol. 2001 Nov;20(7):439-50.
"Selective intracellular retention of extracellular matrix proteins and chaperones associated with pseudoachondroplasia."
Author(s): Vranka J, Mokashi A, Keene DR, Tufa S, Corson G, Sussman M, Horton WA, Maddox K, Sakai L, Bächinger HP
Number of Citations: 17
(See PubMed article )
Immunoprecipitation
  Species / Dilution Summary
  Hu / Not Cited
PA1-027A was used in immunoprecipitation to study the role of cyclophin B in kidney function and the mechanisms of cyclsporin A toxicity.

PLoS One. 2010 Nov 10;5(11):e13930.
"Cyclophilin B interacts with sodium-potassium ATPase and is required for pump activity in proximal tubule cells of the kidney."
Author(s): Suñé G, Sarró E, Puigmulé M, López-Hellín J, Zufferey M, Pertel T, Luban J, Meseguer A
Number of Citations: 1
(See PubMed article )
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