Cyclin A Antibody (6E6)

Cyclin A Monoclonal Antibody for Western blot, IHC (P), IP

>> See 1 other antibody for CCNA1 (cyclin-A1)
Synonyms:
cyclin-A1
Entrez Gene ID:
UniProt ID:
Details
Host / Isotype: Mouse / IgG1, kappa
Class: Monoclonal
Type: Antibody
Clone: 6E6
Tested Species Reactivity: Human (Hu)
Published Species Reactivity: Human (Hu), Non-human primate (Nhp)
Immunogen: Recombinant N-terminal fragment of human cyclin A protein
Ordering Information
Pierce Cyclin A Antibody (6E6)
Product #
MA5-11306
Size
500 ul
Price
$320.00
Purchase
Add Cyclin A Antibody (6E6) to your cart.
Tested Applications Dilution *
Western Blot (WB) 1:100
Immunohistochemistry (Paraffin) (IHC (P)) 1:40
Immunoprecipitation (IP) Assay Dependent
Published Applications Dilution
Western Blot (WB) See publications below
Immunohistochemistry (IHC) See publications below
* Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own experiment using appropriate negative and positive controls.
Form Information
Form: Liquid
Storage Buffer: tissue culture supernatant
Preservative: 0.09% sodium azide
Storage Conditions: 4° C
Product Specific Information
MA5-11306 targets Cyclin A in IHC (P), IP, and WB applications and shows reactivity with Human samples.

The MA5-11306 immunogen is recombinant N-terminal fragment of human cyclin A protein.
General Information
Cyclins are regulatory subunits of the cyclin-dependent kinases (cdk's). They are expressed temporally to control transition at different specific phases of the cell cycle. In mammalian somatic cells, cyclin A is required for S-phase and passage through G2-phase.
Product Images
  • Cyclin A Antibody (MA5-11306) in WB
    MA5-11306_WesternBlot.jpg

    Western Blot with anti-Cyclin A Monoclonal Antibody [6E6] (MA5-11306) (MA5-11306_WesternBlot.jpg)

    Western Blot with anti-Cyclin A Monoclonal Antibody [6E6] (MA5-11306)

    Western blot of Cyclin A using Cyclin A Monoclonal Antibody (MA5-11306) on HeLa Cells.
  • Cyclin A Antibody (MA5-11306) in IHC
    MA5-11306_Immunohistochemistry.jpg

    Immunohistochemistry with anti-Cyclin A Monoclonal Antibody [6E6] (MA5-11306) (MA5-11306_Immunohistochemistry.jpg)

    Immunohistochemistry with anti-Cyclin A Monoclonal Antibody [6E6] (MA5-11306)

    Formalin-fixed, paraffin-embedded human tonsil stained with Cyclin A antibody using peroxidase-conjugate and AEC chromogen. Note nuclear staining of proliferating cells.
  • Cyclin A Antibody (MA5-11306) in IP
    MA5-11306_Immunoprecipitation.jpg

    Immunoprecipitation with anti-Cyclin A Monoclonal Antibody [6E6] (MA5-11306) (MA5-11306_Immunoprecipitation.jpg)

    Immunoprecipitation with anti-Cyclin A Monoclonal Antibody [6E6] (MA5-11306)

    Immunoprecipitation of Cyclin A using Cyclin A Monoclonal Antibody (MA5-11306) on Native Human HeLa Cells.
Publications:
Western Blot
  Species / Dilution Summary
  Hu / 0
MA5-11306 was used in western blot and western blot to investigate the effect of HCV NS2 protein on cell proliferation and cell cycle and its mechanism

Virus Res. 2006 Nov;121(2):134-43.
"HCV NS2 protein inhibits cell proliferation and induces cell cycle arrest in the S-phase in mammalian cells through down-regulation of cyclin A expression."
Author(s): Yang XJ, Liu J, Ye L, Liao QJ, Wu JG, Gao JR, She YL, Wu ZH, Ye LB
Number of Citations: 1
(See PubMed article )
  Hu / 0
MA5-11306 was used in western blot to investigate a novel adenoviral method of gene therapy against human colon carcinoma xenografts

Cancer Res. 2007 May 15;67(10):4949-55.
"Suicide gene therapy of human colon carcinoma xenografts using an armed oncolytic adenovirus expressing carboxypeptidase G2."
Author(s): Schepelmann S, Ogilvie LM, Hedley D, Friedlos F, Martin J, Scanlon I, Chen P, Marais R, Springer CJ
Number of Citations: 1
(See PubMed article )
  Hu / 0
MA5-11306 was used in western blot to study novel human Cdt1-binding proteins using a proteomics approach

Mol Biol Cell. 2008 Mar;19(3):1007-21.
"Identification of novel human Cdt1-binding proteins by a proteomics approach: proteolytic regulation by APC/CCdh1."
Author(s): Sugimoto N, Kitabayashi I, Osano S, Tatsumi Y, Yugawa T, Narisawa-Saito M, Matsukage A, Kiyono T, Fujita M
Number of Citations: 14
(See PubMed article )
  Nhp / 0
MA5-11306 was used in western blot and western blot to investigate the effect of HCV NS2 protein on cell proliferation and cell cycle and its mechanism

Virus Res. 2006 Nov;121(2):134-43.
"HCV NS2 protein inhibits cell proliferation and induces cell cycle arrest in the S-phase in mammalian cells through down-regulation of cyclin A expression."
Author(s): Yang XJ, Liu J, Ye L, Liao QJ, Wu JG, Gao JR, She YL, Wu ZH, Ye LB
Number of Citations: 1
(See PubMed article )
Immunohistochemistry
  Species / Dilution Summary
  Hu / 1:100
MA5-11306 was used in immunohistochemistry to study the expression and role of retinoblastoma protein/p107 and retinoblastoma protein2/p130 in non-Hodgkin's lymphoma

Clin Cancer Res. 1999 Dec;5(12):4065-72.
"Retinoblastoma-related p107 and pRb2/p130 proteins in malignant lymphomas: distinct mechanisms of cell growth control."
Author(s): Leoncini L, Bellan C, Cossu A, Claudio PP, Lazzi S, Cinti C, Cevenini G, Megha T, Laurini L, Luzi P, Orcioni GF, Piccioli M, Pileri S, Giardino C, Tosi P, Giordano A
Number of Citations: 8
(See PubMed article )
  Hu / 1:20
MA5-11306 was used in immunohistochemistry to study the utility of human EGF 2 and cyclin A2 in predicting recurrence of invasive breast cancer

Chin Med J (Engl). 2010 Feb 20;123(4):431-7.
"Prediction of recurrence risk in early breast cancer using human epidermal growth factor 2 and cyclin A2."
Author(s): Li HP, Ji JF, Hou KY, Lei YT, Zhao HM, Wang J, Zheng J, Liu JY, Wang MP, Xiao Y, Wang YF, Lü YY, Sun Y
Number of Citations: 1
(See PubMed article )
  Hu / 1:100
MA5-11306 was used in immunohistochemistry to study the diagnostic value of cyclin A and cyclin D1 expression in differentiated thyroid carcinoma

Med Oncol. 2012 Mar;29(1):294-300.
"Cyclin A and cyclin B1 overexpression in differentiated thyroid carcinoma."
Author(s): Nar A, Ozen O, Tutuncu NB, Demirhan B
Number of Citations: 1
(See PubMed article )
(This product is for In Vitro experimental use only. Not for resale without express authorization.)
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