Cyclin A Antibody (6E6)

Cyclin A Monoclonal Antibody for Western blot, IHC (P), IP

>> See 1 other antibody for CCNA1 (cyclin-A1)
Synonyms:
cyclin-A1
Entrez Gene ID:
UniProt ID:
Details
Host / Isotype: Mouse / IgG1, kappa
Class: Monoclonal
Type: Antibody
Clone: 6E6
Tested Species Reactivity: Human (Hu)
Published Species Reactivity: Human (Hu), Non-human primate (Nhp)
Immunogen: Recombinant N-terminal fragment of human cyclin A protein
Ordering Information
Pierce Cyclin A Antibody (6E6)
Product #
MA5-11306
Size
500 ul
Price
$320.00
Purchase
Add Cyclin A Antibody (6E6) to your cart.
Tested Applications Dilution *
Western Blot (WB) 1:100
Immunohistochemistry (Paraffin) (IHC (P)) 1:40
Immunoprecipitation (IP) Assay Dependent
Published Applications Dilution
Western Blot (WB) See publications below
Immunohistochemistry (IHC) See publications below
* Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own experiment using appropriate negative and positive controls.
Form Information
Form: Liquid
Storage Buffer: tissue culture supernatant
Preservative: 0.09% sodium azide
Storage Conditions: 4° C
Product Specific Information
MA5-11306 targets Cyclin A in IHC (P), IP, and WB applications and shows reactivity with Human samples.

The MA5-11306 immunogen is recombinant N-terminal fragment of human cyclin A protein.
General Information
Cyclins are regulatory subunits of the cyclin-dependent kinases (cdk's). They are expressed temporally to control transition at different specific phases of the cell cycle. In mammalian somatic cells, cyclin A is required for S-phase and passage through G2-phase.
Product Images
  • Cyclin A Antibody (MA5-11306) in WB
    MA5-11306_WesternBlot.jpg

    Western Blot with anti-Cyclin A Monoclonal Antibody [6E6] (MA5-11306) (MA5-11306_WesternBlot.jpg)

    Western Blot with anti-Cyclin A Monoclonal Antibody [6E6] (MA5-11306)

    Western blot of Cyclin A using Cyclin A Monoclonal Antibody (MA5-11306) on HeLa Cells.
  • Cyclin A Antibody (MA5-11306) in IHC
    MA5-11306_Immunohistochemistry.jpg

    Immunohistochemistry with anti-Cyclin A Monoclonal Antibody [6E6] (MA5-11306) (MA5-11306_Immunohistochemistry.jpg)

    Immunohistochemistry with anti-Cyclin A Monoclonal Antibody [6E6] (MA5-11306)

    Formalin-fixed, paraffin-embedded human tonsil stained with Cyclin A antibody using peroxidase-conjugate and AEC chromogen. Note nuclear staining of proliferating cells.
  • Cyclin A Antibody (MA5-11306) in IP
    MA5-11306_Immunoprecipitation.jpg

    Immunoprecipitation with anti-Cyclin A Monoclonal Antibody [6E6] (MA5-11306) (MA5-11306_Immunoprecipitation.jpg)

    Immunoprecipitation with anti-Cyclin A Monoclonal Antibody [6E6] (MA5-11306)

    Immunoprecipitation of Cyclin A using Cyclin A Monoclonal Antibody (MA5-11306) on Native Human HeLa Cells.
Publications:
Western Blot
  Species / Dilution Summary
  Hu / 0
MA5-11306 was used in western blot and western blot to investigate the effect of HCV NS2 protein on cell proliferation and cell cycle and its mechanism

Virus Res. 2006 Nov;121(2):134-43.
"HCV NS2 protein inhibits cell proliferation and induces cell cycle arrest in the S-phase in mammalian cells through down-regulation of cyclin A expression."
Author(s): Yang XJ, Liu J, Ye L, Liao QJ, Wu JG, Gao JR, She YL, Wu ZH, Ye LB
Number of Citations: 1
(See PubMed article )
  Hu / 0
MA5-11306 was used in western blot to investigate a novel adenoviral method of gene therapy against human colon carcinoma xenografts

Cancer Res. 2007 May 15;67(10):4949-55.
"Suicide gene therapy of human colon carcinoma xenografts using an armed oncolytic adenovirus expressing carboxypeptidase G2."
Author(s): Schepelmann S, Ogilvie LM, Hedley D, Friedlos F, Martin J, Scanlon I, Chen P, Marais R, Springer CJ
Number of Citations: 1
(See PubMed article )
  Hu / 0
MA5-11306 was used in western blot to study novel human Cdt1-binding proteins using a proteomics approach

Mol Biol Cell. 2008 Mar;19(3):1007-21.
"Identification of novel human Cdt1-binding proteins by a proteomics approach: proteolytic regulation by APC/CCdh1."
Author(s): Sugimoto N, Kitabayashi I, Osano S, Tatsumi Y, Yugawa T, Narisawa-Saito M, Matsukage A, Kiyono T, Fujita M
Number of Citations: 14
(See PubMed article )
  Nhp / 0
MA5-11306 was used in western blot and western blot to investigate the effect of HCV NS2 protein on cell proliferation and cell cycle and its mechanism

Virus Res. 2006 Nov;121(2):134-43.
"HCV NS2 protein inhibits cell proliferation and induces cell cycle arrest in the S-phase in mammalian cells through down-regulation of cyclin A expression."
Author(s): Yang XJ, Liu J, Ye L, Liao QJ, Wu JG, Gao JR, She YL, Wu ZH, Ye LB
Number of Citations: 1
(See PubMed article )
Immunohistochemistry
  Species / Dilution Summary
  Hu / 1:20
MA5-11306 was used in immunohistochemistry to study the utility of human EGF 2 and cyclin A2 in predicting recurrence of invasive breast cancer

Chin Med J (Engl). 2010 Feb 20;123(4):431-7.
"Prediction of recurrence risk in early breast cancer using human epidermal growth factor 2 and cyclin A2."
Author(s): Li HP, Ji JF, Hou KY, Lei YT, Zhao HM, Wang J, Zheng J, Liu JY, Wang MP, Xiao Y, Wang YF, Lü YY, Sun Y
Number of Citations: 1
(See PubMed article )
(This product is for In Vitro experimental use only. Not for resale without express authorization.)
Part of Thermo Fisher Scientific