Part of Thermo Fisher Scientific

Complement C1s Monoclonal Antibody (49)

Complement C1s Antibody for ELISA

Synonyms:
C1 esterase, Complement C1s subcomponent,
Entrez Gene ID:
(Human) 716
UniProt ID:
P09871
Details
Host / Isotype: Mouse / IgG1, kappa
Class: Monoclonal
Type: Antibody
Clone: 49
Species Reactivity: Human (Hu)
Immunogen: Human plasma C1s Proenzyme.
Ordering Information
Pierce Complement C1s Monoclonal Antibody (49)
Product Number Pkg. Size Price Purchase
ABS 002-49-02 200 µg $362.00
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Storage: 4º C, store in dark
Form: 200 µg of Protein A/G purified antibody (1 mg/ml) in 0.01 M phosphate buffer, with 0.5 M NaCl and 15 mM sodium azide; pH 7.4.


Applications Dilution *
ELISA (ELISA) 1:1,000
* Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own experiment using appropriate negative and positive controls.
Product Specific Information
ABS 002-49-02 is specific for C1s proenzyme and activated free C1s and C1s in complex with C1 inhibitor.

ABS 002-49-02 has been successfully used in ELISA procedures. ABS 002-49-02 reacts in ELISA when tested with activated C1s enzyme directly coated onto the microtiter well or in sandwich ELISA in combination with a polyclonal antibody in coat.

The ABS 002-49-02 immunogen is Human plasma C1s Proenzyme.

Epitope of ABS 002-49-02 differs from that of ABS 002-09-02.
General Information
C1s proenzyme is a 79.8 kDa glycoprotein produced primarily by hepatocytes (1) but also by other cells (2,3). The proenzyme is produced as a single-chain polypeptide of 688 aa. Upon activation the peptide is cleaved between R437 and I438 giving the activated enzyme consisting of two disulfide-linked chains, A chain (52.2 kDa) and B chain (27.7 kDa). The Ca-dependent catalytic subunit of the C1 complex (C1q, C1r, C1s) comprises of two C1s and two C1r molecules and this serine protease tetramer is responsible for the initial steps in the classical complement pathway, cleavage of C4 and C2.
PubMed References:
Blocking Assay
  Species / Dilution Summary
  Hu / 1 mg/ml
 ABS 002-49-02 was used in blocking/activating experiment and ELISA to characterize human TSH receptor antibodies in vitro and in vivo
Endocrinology. 1995 Jul;136(7):2817-24.
"Generation and characterization of monoclonal antibodies to the human thyrotropin (TSH) receptor: antibodies can bind to discrete conformational or linear epitopes and block TSH binding."
Author(s): Seetharamaiah GS, Wagle NM, Morris JC, Prabhakar BS
Number of Citations: 3
(See PubMed article )
ELISA
  Species / Dilution Summary
  Hu / 12.5-50 ug/well
 ABS 002-49-02 was used in blocking/activating experiment and ELISA to characterize human TSH receptor antibodies in vitro and in vivo
Endocrinology. 1995 Jul;136(7):2817-24.
"Generation and characterization of monoclonal antibodies to the human thyrotropin (TSH) receptor: antibodies can bind to discrete conformational or linear epitopes and block TSH binding."
Author(s): Seetharamaiah GS, Wagle NM, Morris JC, Prabhakar BS
Number of Citations: 3
(See PubMed article )
  Hu / Not Cited
Ms / Not Cited
 ABS 002-49-02 was used in ELISA and western blot to investigate the expression of mET-gp and characterize the difference of epitopes between mET-gp and ETSHr-gp
Endocrinology. 1997 Apr;138(4):1559-66.
"Differential reactivities of recombinant glycosylated ectodomains of mouse and human thyrotropin receptors with patient autoantibodies."
Author(s): Patibandla SA, Seetharamaiah GS, Dallas JS, Thotakura NR, Peake RL, Prabhakar BS
Number of Citations: 1
(See PubMed article )
Western Blot
  Species / Dilution Summary
  Hu / 1:100
 ABS 002-49-02 was used in ELISA and western blot to investigate the expression of mET-gp and characterize the difference of epitopes between mET-gp and ETSHr-gp
Endocrinology. 1997 Apr;138(4):1559-66.
"Differential reactivities of recombinant glycosylated ectodomains of mouse and human thyrotropin receptors with patient autoantibodies."
Author(s): Patibandla SA, Seetharamaiah GS, Dallas JS, Thotakura NR, Peake RL, Prabhakar BS
Number of Citations: 1
(See PubMed article )
(This product is for In Vitro experimental use only. Not for resale without express authorization.)