Part of Thermo Fisher Scientific

Collagen V Monoclonal Antibody (1E2-E4/Col5)

Collagen V Antibody for ELISA, IHC, Western blot

Synonyms:
COL5, AB collagen, Collagen fetal membrane A polypeptide, Collagen V alpha 2 polypeptide
Entrez Gene ID:
(Human) 1289
UniProt ID:
P20908
Details
Host / Isotype: Mouse / IgG2a
Class: Monoclonal
Type: Antibody
Clone: 1E2-E4/Col5
Species Reactivity: Human (Hu) Marsupial (Mr) Canine (Ca) Rabbit (Rb) Bovine (Bv) Ovine (Ov) Porcine (Po)
Immunogen: Acid-digested pepsin soluble dog type V collagen.
Ordering Information
Pierce Collagen V Monoclonal Antibody (1E2-E4/Col5)
Product Number Pkg. Size Price Purchase
CSI 006-01-02 200 µg $402.00
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Storage: 4º C, store in dark
Form: 200 µg of Protein A/G purified antibody (1 mg/ml) in 0.01 M phosphate buffer, with 0.5 M NaCl and 15 mM sodium azide; pH 7.4.


Applications Dilution *
Immunohistochemistry (IHC) Assay dependent
Western Blot (WB) Assay dependent
ELISA (ELISA) 1:50
* Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own experiment using appropriate negative and positive controls.
Product Specific Information
CSI 006-01-02 is highly specific for type V collagen. It has been shown to have no cross-reactivity with type I, III and VI collagens by ELISA and immunoblotting. There is no evidence for cross-reactivity with other connective tissue proteins (laminin, fibronectin, elastin). CSI 006-01-02 shows reactivity with human, dog, calf, sheep, pig and kangaroo in type V collagens by ELISA and immunoblotting. CSI 006-01-02 does not show reactivity with mouse, rat, guinea pig and chicken type V collagens by ELISA and immunoblotting.
 
CSI 006-01-02 has been successfully used in Western blotting, ELISA and Immunohistochemistry procedures. CSI 006-01 has been used succesfully for immunohistology on frozen unfixed sections of human, bovine and dog skin, of foetal bovine cornea, and of new dog tissue associated with a biomaterial implant. If fixation of tissue is required, acetone or ethanol is recommended. CSI 006-01 binds poorly to Collagen V when tested in ELISA with Collagen V coated directly onto the microtiter well.

 The CSI 006-01-02 immunogen is acid-digested pepsin soluble dog type V collagen.
General Information
Type V collagen is a minor component of the connective tissue, although it is present in many different types of connective tissue. Patients with defects in the type V collagen (Ehlers-Danlos syndrome) have weakend connective tissue characterized by hyperstrechable joints and fragile, easily bruisable skin.
(This product is for In Vitro experimental use only. Not for resale without express authorization.)