Part of Thermo Fisher Scientific

Collagen III Monoclonal Antibody (1E7-D7/Col3)

Collagen III Antibody for ELISA, IHC (F), Western blot

Synonyms:
COL3, Collagen type III alpha 1, EDS4A
Entrez Gene ID:
(Human) 1281
UniProt ID:
P02461
Details
Host / Isotype: Mouse / IgG1, kappa
Class: Monoclonal
Type: Antibody
Clone: 1E7-D7/Col3
Species Reactivity: Human (Hu) Marsupial (Mr) Rat (Rt) Canine (Ca)
Immunogen: Acid-digested pepsin soluble human type III collagen.
Ordering Information
Pierce Collagen III Monoclonal Antibody (1E7-D7/Col3)
Product Number Pkg. Size Price Purchase
CSI 007-01-02 200 µg $426.00
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Storage: 4º C, store in dark
Form: 200 µg of Protein A/G purified antibody (1 mg/ml) in 0.01 M phosphate buffer, with 0.5 M NaCl and 15 mM sodium azide; pH 7.4.


Applications Dilution *
ELISA (ELISA) 1:800
Western Blot (WB) Assay dependent
Immunohistochemistry (Frozen) (IHC (F)) Assay dependent
* Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own experiment using appropriate negative and positive controls.
Product Specific Information
CSI 007-01-02 is highly specific for type III collagen. It has been shown to have no cross-reactivity with type I and V collagens by ELISA and immunoblotting. There is no evidence for cross-reactivity with other connective tissue proteins (laminin, fibronectin, elastin). CSI 007-01-02 shows reactivity with human, dog, rat and kangaroo in type III collagens by ELISA and immunoblotting. The antibody does not show reactivity with calf and sheep type III collagen by ELISA and immunoblotting.
 
CSI 007-01-02 has been successfully used in Western blotting, ELISA, and immunohistochemistry (frozen) procedures. CSI 007-01 has been used successfully for immunohistology on frozen unfixed sections of human and kangaroo skin, and of new dog tissue associated with biomaterial implants. If fixation of tissue is required, acetone or ethanol is recommended.
 
The CSI 007-01-02 immunogen is acid-digested pepsin soluble human type III collagen.

The specificity of the CSI 007-01-02 epitope is located approximately 100nm from the N-terminal of the molecule.
General Information
Type III collagen is present in the walls of arteries, intestines and the uterus, where it strenghtens the connective tissue. Patients with defects in the type III collagen (ex. Ehlers-Danlos syndrome) are at risk of rupture of major arteries or the intestine.
(This product is for In Vitro experimental use only. Not for resale without express authorization.)